全文获取类型
收费全文 | 9918篇 |
免费 | 561篇 |
国内免费 | 1023篇 |
专业分类
林业 | 1056篇 |
农学 | 1345篇 |
基础科学 | 929篇 |
1567篇 | |
综合类 | 2975篇 |
农作物 | 692篇 |
水产渔业 | 453篇 |
畜牧兽医 | 1514篇 |
园艺 | 382篇 |
植物保护 | 589篇 |
出版年
2024年 | 43篇 |
2023年 | 134篇 |
2022年 | 329篇 |
2021年 | 500篇 |
2020年 | 413篇 |
2019年 | 429篇 |
2018年 | 296篇 |
2017年 | 435篇 |
2016年 | 348篇 |
2015年 | 467篇 |
2014年 | 478篇 |
2013年 | 522篇 |
2012年 | 642篇 |
2011年 | 682篇 |
2010年 | 609篇 |
2009年 | 554篇 |
2008年 | 553篇 |
2007年 | 521篇 |
2006年 | 503篇 |
2005年 | 419篇 |
2004年 | 221篇 |
2003年 | 210篇 |
2002年 | 225篇 |
2001年 | 223篇 |
2000年 | 238篇 |
1999年 | 214篇 |
1998年 | 159篇 |
1997年 | 153篇 |
1996年 | 161篇 |
1995年 | 132篇 |
1994年 | 104篇 |
1993年 | 119篇 |
1992年 | 101篇 |
1991年 | 85篇 |
1990年 | 62篇 |
1989年 | 59篇 |
1988年 | 42篇 |
1987年 | 35篇 |
1986年 | 25篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
本文试以北京金中都公园设计和建设过程为例,论述如何将园林景观与历史文化的合理展示相结合。提出设计师的创作和实践应该与建设过程相配合,以匠人精神应对施工阶段的现场完形和设计提升。总结了设计项目从概念规划直至建成,设计师的全过程设计的重要性和必要性。探讨了景观与艺术专业的协同设计在文化表达和景观展示中的叠加效应。提出设计师对场地的挖掘和设计推敲,应该随着项目的施工建设进度并以现场设计为核心,并举例论述了现场调整对提升设计品质的重要作用。以期为公园改造和园林景观设计师的工作实践提供有益的参考和引导。 相似文献
62.
韩宇红 《农产品加工.学刊》2011,(9):48-51,64
随着ECFA的实施,漳台(漳州市、台湾省,下同)农业资源将进一步得到合理配置,漳台农业交流合作的空间和领域将得到更大的拓展。漳州市如何在现有的基础和成效上抓住这些有利时机,突破制约漳台农业进一步合作的瓶颈,努力促进漳台农业合作实现跨越式发展,具有重大的现实意义和深远的历史意义。 相似文献
63.
64.
ZHANG Han MA Jing ZHANG Yun-ling ZHANG Shu-ming XU Qing-rui WANG Wei-ming 《园艺学报》2015,31(12):2244-2248
AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells. 相似文献
65.
介绍了一种农机车载电子设备显示界面框架的设计与实现。基于C语言分析设计了界面框架的数据结构,使用分层的数据结构设计方法实现了多类型数据的显示需要和灵活的界面类型组合方式,以极少的开销实现了多级菜单。将内容数据与操作函数分离,解决了操作函数复用的问题,同时提高了程序的可维护性。提出的新型的界面显示框架实现了多种农业装备常见的界面应用类型,解决了小屏幕嵌入式系统显示界面移植性差问题。 相似文献
66.
总结回顾浙江省食用菌发展的历史和取得的成就,指出食用菌是浙江省农业十大主导优势产业之一,食用菌栽培种类全,分布区域集中,规模化生产水平较高;在科学技术进步、农业丰收、技术改造、品牌建设、国家地理标志等方面获得了诸多成果与荣誉,涌现出一大批优秀的行业先锋;最早提出并制定一系列食用菌标准化生产规范,并注重“菌文化”的传承。浙江食用菌面临的主要困难有:发展动力减弱,生产规模下滑;食用菌产业科技支撑不足,缺少自主核心技术;品牌以及消费宣传不够,缺乏正确的饮食引导。提出具体发展建议。 相似文献
67.
68.
Xiaosha Li Huifang Han Tangyuan Ning Ying Shen Rattal Lal 《Soil Use and Management》2019,35(4):585-594
To assess changes in organic carbon pools, an incubation experiment was conducted under different temperatures and field moisture capacity (FMC) on a brown loam soil from three tillage practices used for 12 years: no‐till (NT), subsoiling (ST) and conventional tillage (CT). Total microbial respiration was measured for incubated soil with and without the input of straw. Results indicated that soil organic carbon (SOC) and microbial biomass carbon (MBC) under ST, NT and CT was higher in soil with straw input than that without, while the microbial quotient (MQ or MBC: SOC) and metabolic quotient (qCO2) content under CT followed the opposite trend. Lower temperature, lower moisture and with straw input contributed to the increases in SOC concentration, especially under NT and ST systems. The SOC concentrations under ST, with temperatures of 30 and 35°C after incubation at 55% FMC, were greater than those under CT by 28.4% and 30.6%, respectively. The increase in MBC was highest at 35°C for 55%, 65% and 75% FMC; in soil under ST, MBC was greater than that under CT by 199.3%, 50.7% and 23.8%, respectively. At 30°C, the lower qCO2 was obtained in soil incubated under NT and ST. The highest MQ among three tillage practices was measured under ST at 55% FMC, NT at 65% FMC and CT at 75% FMC with straw input. These data indicate the benefits of enhancing the MQ; the low FMC was beneficial to ST treatment. Under higher temperature and drought stress conditions, the adaptive capacity of ST and NT is better than that of CT. 相似文献
69.
为验证自行研制的A型流感病毒分型基因芯片方法在实际中的应用效果,了解A型流感病毒各亚型在我国不同地区和不同宿主中的分布情况,对我国20个地区、不同宿主源(鸡、鸭、鹌鹑、鸽子、猪、人等)拭子和组织样品共16 852份进行了检测。利用自行研制的A型流感病毒分型基因芯片方法,对样品进行RNA提取、多重不对称RT-PCR扩增、芯片杂交、洗涤和扫描分析;同时将基因芯片检测阳性的样品,用普通RT-PCR扩增后进行序列测定。结果显示:利用该方法检出阳性样品2 582份,总检出率为15.32%(2 582/16 852),共检测到H1N1、H2N9、H3N2、H4N6、H5N1、H7N1、H7N9、H9N2、H10N5、H16N3等10个亚型,且均与阳性样品测序结果一致。结果表明,该方法可准确检测不同地区、不同宿主中的A型流感病毒不同亚型。同时,该方法能在同一张芯片上准确鉴定A型流感病毒的多种亚型,解决了其他常规方法无法检测已发现和可能发生变异的所有亚型的棘手问题,从而为A型流感诊断和分子流行病学监测提供了一种新技术。 相似文献
70.
[Objective] Dimorphic InDel markers can be used for cotton variety identification and purity detection, to improve the accuracy and efficiency of cotton seed testing, and to play a role in molecular breeding of cotton. [Method] Based on the whole genome sequencing of 121 cotton varieties from different sources, the InDel markers with high polymorphism were developed according to polymorphism information content(PIC) and were applied in the genetic distance analysis and cluster analysis by using 66 cotton varieties in China. [Result] Totally 10 967 InDel were identified based on the next generation sequencing data of 121 cotton varieties. Among the 85 pairs of InDel primers synthesized, 64 were selected including 35 from At group and 29 from Dt group. The minimum average allelic frequency(MAF) of At and Dt chromosomes were 0.45 and 0.32, respectively, while the PIC were 0.49 and 0.40, respectively. The genetic distances of the 66 cotton varieties ranged from 0.04 to 0.65 centimorgan (cM), with an average of 0.39 cM. The two varieties with the largest genetic distance were Simian 3 and CCRI 36, and the two varieties with the smallest genetic distance were Xumian 18 and Xuza 3. [Conclusion] The 64 cotton dimorphic InDel markers can effectively reveal the relationships among varieties based on the genetic distance, and distinguish cotton varieties from different sources, which has certain theoretical significance and application value. 相似文献