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91.
Previously, we determined that a primary cause of proportional stunted growth in a line of Brahman cattle was related to an apparent refractoriness in metabolic response to GH in young animals. The objective of this study was to determine the effect of administration of GH, insulin (INS), and GH plus INS to mature miniature Brahman cows (n = 6; 9.7 ± 2.06 y; 391 ± 48.6 kg) and bulls (n = 8; 9.4 ± 2.00 y; 441 ± 54.0 kg) on circulating concentrations of metabolic hormones and metabolites, primarily IGF-I and IGF-I binding proteins. We hypothesized that IGF-I secretion could be enhanced by concomitant administration of exogenous GH and INS, and neither alone would be effective. Animals were allotted to a modified crossover design that included four treatments: control (CON), GH, INS, and GH + INS. At the start of the study, one-half of the cattle were administered GH (Posilac; 14-d slow release) and the other one-half served as CON for 7 d. Beginning on day 8, and for 7 d, INS (Novolin L) was administered (0.125 IU/kg BW) twice daily (7:00 AM and 7:00 PM) to all animals; hence, the INS and GH + INS treatments. Cattle were rested for 14 d and then were switched to the reciprocal crossover treatments. Blood samples were collected at 12-hour intervals during the study. Compared with CON, GH treatment increased (P < 0.01) mean plasma concentrations of GH (11.1 vs 15.7 ± 0.94 ng/mL), INS (0.48 vs 1.00 ± 0.081 ng/mL), IGF-I (191.3 vs 319.3 ± 29.59 ng/mL), and glucose (73.9 vs 83.4 ± 2.12 mg/dL) but decreased (P < 0.05) plasma urea nitrogen (14.2 vs 11.5 ± 0.75 mg/dL). Compared with INS, GH + INS treatment increased (P < 0.05) mean plasma concentration of INS (0.71 vs 0.96 ± 0.081 ng/mL), IGF-I (228.7 vs 392.3 ± 29.74 ng/mL), and glucose (48.1 vs 66.7 ± 2.12 mg/dL), decreased (P < 0.01) plasma urea nitrogen (13.6 vs 10.4 ± 0.76 mg/dL), and did not affect GH (13.5 vs 12.7 ± 0.95 ng/mL). In the miniature Brahman model, both the GH and GH + INS treatments dramatically increased circulating concentrations of IGF-I in mature cattle, suggesting that this line of Brahman cattle is capable of responding to bioactive GH.  相似文献   
92.
Before 2007, equine influenza had never been diagnosed in Australia. On 22 August 2007, infection was confirmed in horses at Eastern Creek Animal Quarantine Station near Sydney. The virus subsequently isolated (A/equine/Sydney/2888-8/2007) was confirmed by sequence analysis of the haemagglutinin (HA) gene as an H3 virus of the variant American Florida lineage that is now referred to as Clade 1. The HA sequence of the virus was identical to that of a virus isolated from a contemporaneous outbreak in Japan and showed high homology to viruses circulating in North America.  相似文献   
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Coastal Chip is a medium-late maturing potato chipping variety suitable for production in locations along the east coast of the United States where heat stress reduces internal quality of tubers. It yields as well as the variety Atlantic but is not as susceptible to heat necrosis. The specific gravity of Coastal Chip averages approximately 0.010 less than Atlantic at locations subject to heat stress. In the seed producing areas of Maine it’s specific gravity averages approximately 0.005 less than that of Atlantic. Tubers are round, netted-skinned, and have moderately deep stem and bud ends. Chips from Coastal Chip are slightly lighter in color than those produced by Atlantic. In mid-Atlantic states where heat stress adversely affects chip color Coastal Chip processes into chips for a slightly longer period of time after harvest than does Atlantic. Glycoalkaloid content of Coastal Chip averaged 7.9 mg/100 g fresh tissue. Sunburned (green) tubers of Coastal Chip develop purple streaks that remain following processing. Hills must be properly covered to reduce losses. Coastal Chip is resistant to race A of the golden nematode (Globodera rostochiensis) and potato virus A. It is tolerant to Verticillium wilt, more susceptible to common scab and Rhizoctonia than Atlantic and susceptible to potato virus X.  相似文献   
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 Worsening water scarcity will increase pressure to use water more productively. In the classical view of irrigation research, some important aspects are often ignored: the total water balance approach, productivity of water, food security, and irrigation-system level analyses. These four approaches were evaluated using a detailed agro-hydrological model applied to an irrigation system in western Turkey. Emphasis was placed on the two dominant crops in the area: cotton and grapes. According to the classical point of view, the only result would be to irrigate the cotton with 1000 mm and the grapes with 800 mm. From the water productivity point of view, however, the water productivity of grapes appeared to be maximal without any irrigation; while for the cotton, irrigation at 600 mm maximizes water productivity. To minimize risks and increase yield stability, grapes perform better than cotton. Finally, from the irrigation system point of view, decisions can be made about the desirable cropping pattern and the distribution of water between crops. With limited amounts of water available for irrigation, a cropping pattern consisting mainly of grapes is desired; while with higher water availability, a mixture of cotton and grapes is preferable. The methods presented provide a clear methodology with which to achieve the most productive use of water. Received: 3 June 1999  相似文献   
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The virulence of a strain of hog cholera virus isolated during an outbreak of mild disease in pigs in New South Wales in 1960/61 (the NSW strain) was compared over 11 days with that of a virulent strain by inoculating 8 pigs with each virus and comparing the ensuing clinical signs and pathology. Both viruses caused persistent pyrexia and leukopenia, the NSW strain 4 to 5 days and the virulent strain 3 days, after inoculation. Few other clinical signs were observed in the pigs inoculated with the NSW strain. In contrast, all pigs inoculated with the virulent strain became progressively depressed and incoordinated, and were killed between days 6 and 9. Bronchopneumonia and swollen, reddened lymph nodes were observed in pigs inoculated with both viruses. Few other gross lesions were observed with the NSW strain, but some pigs receiving the virulent strain had pustules in the tonsil and the anterior oesophagus, petechial haemorrhages in the kidney, and small infarcts at the margins of the spleen. There were marked differences in the histopathology, both in the variety of organs affected and the severity of lesions in individual organs. Suppurative bronchopneumonia occurred in both groups. Other changes in the pigs affected with the NSW strain were colitis, mild cerebral vasculitis, necrosis, haemorrhage and neutrophil infiltration in some lymph nodes and spleens. In pigs infected with virulent virus the cerebral vasculitis was so severe that there was necrosis of cells within the vessel walls.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   
100.
Susceptibility of cats to equine morbillivirus   总被引:7,自引:0,他引:7  
Objective To assess the susceptibility of cats to equine morbillivirus (EMV) by direct administration of the virus by subcutaneous, intra-nasal or oral routes, and following exposure to infected cats.
Design A disease transmission study, with controls, using ten cats.
Procedure Groups of cats were given the virus by the designated methods and assessed for evidence of infection by clinical examination, plus pathological and virological tests.
Results All cats administered the virus by subcutaneous, intra-nasal or oral routes became infected and developed the disease within 4 to 8 days. One of two cats in contact with affected cats also developed the disease, but two cats kept near to affected cats did not become infected. The virus was isolated from a range of tissues collected from the infected cats, and the lesions observed in affected cats were similar to those previously observed in horses naturally and experimentally infected with the virus.
Conclusion This is the first demonstration that animals can be infected with EMV by non-parenteral means, that the virus can transmit naturally between animals and confirms other reports of the similarity of EMV disease in horses and cats.  相似文献   
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