Expression of interleukin (IL)-5 and IL-9 receptors on neutrophils of horses with heaves

Heaves, a condition associated with airway neutrophilia, is believed to result from an allergic response to environmental dust particles. However, the contribution of neutrophils to the allergic response is poorly understood. It has been hypothesized that Th2-type cytokines can directly activate neutrophils to produce pro-inflammatory mediators. The present study focused on the presence of receptors for the Th2-type cytokines interleukin (IL)-5 and IL-9 on peripheral blood neutrophils of horses with heaves. Neutrophils were isolated from peripheral blood of horses with heaves (n=7), and normal control (n=5) before (pasture) and 3 weeks following a continuous natural allergen challenge (stabling). Horses with heaves had significantly increased numbers of neutrophils expressing IL-5 and IL-9 receptors compared to control while in pasture, and further increased during stabling in heaves affected horses but not in control animals. These results provide a possible mechanism by which Th2-type cytokines may activate neutrophils in equine heaves.     

Carbon isotope discrimination and water use efficiency in Iranian diploid,tetraploid and hexaploid wheats grown under well-watered conditions

Carbon isotope discrimination (Δ) has been proposed as physiological criterion to select C₃ crops for yield and water use efficiency. The relationships between carbon isotope discrimination (Δ), water use efficiency for grain and biomass production (WUE_G and WUE_B, respectively) and plant and leaf traits were examined in 20 Iranian wheat genotypes including einkorn wheat (Triticum monococcum L. subsp. monococcum) accessions, durum wheat (T. turgidum L. subsp. durum (Desf.) Husn.) landraces and bread wheat (T. aestivum L. subsp. aestivum) landraces and improved cultivars, grown in pots under well-watered conditions. Carbon isotope discrimination was higher in diploid than in hexaploid and tetraploid wheats and was negatively associated with grain yield across species as well as within bread wheat. It was also positively correlated to stomatal frequency. The highest WUE_G and grain yield were noted in bread wheat and the lowest in einkorn wheat. Einkorn and bread wheat had higher WUE_B and biomass than durum wheat. WUE_G and WUE_B were significantly negatively associated to Δ across species as well as within bread and durum wheat. The variation for WUE_G was mainly driven by the variation for harvest index across species and by the variation for Δ within species. The quantity of water extracted by the crop, that was closely correlated to root mass, poorly influenced WUE_G. Environmental conditions and genetic variation for water use efficiency related traits appear to highly determine the relationships between WUE_G and its different components (water consumed, transpiration efficiency and carbon partitioning).     

The effect of multiplex-PCR-assessed major pathogens causing subclinical mastitis on somatic cell profiles

The major pathogens causing mastitis were evaluated by multiplex-polymerase chain reaction (M-PCR) with self-designed primers in four quarters of the first, third, and fifth parities in industrial, semi-industrial, and traditional dairy cattle farms in Iran. With the incidence of infection in the quarters by Staphylococcus aureus and Streptococcus agalactiae, the mean log somatic cell count (log SCC) increased from 5.06 to 5.77. The smallest changes occurred with Escherichia coli. Contagious pathogens, when compared with environmental pathogens, were more prevalent and common and created more profound quantitative and qualitative changes in SCC profiles. The second part of the study surveyed the diversity of contaminating pathogens and their effect on quantitative and qualitative profiles of somatic cells. M-PCR was used to determine the absence (M-PCR(-)) and presence of one (M-PCR(+1)), two (M-PCR(+2)), and three (M-PCR(+3)) major pathogens in raw milk samples. Quarter log SCC increased from 5.06 (for M-PCR(-1)) to 5.5 (for M-PCR(+1)), 5.7 (for M-PCR(+2)), and 6 (for M-PCR(+3)). Percent changes in polymorphonuclears (PMNs) were not significant between different quarters and parities but were significant between different farms in terms of pathogen diversity (P?相似文献   

Fire-retarding properties of nano-silver in solid woods

Fire-retarding properties of 200?ppm nano-silver solution, with a size ranging from 20 to 80?nm, on five woody species were studied and compared with Borax and Celcune ^? . Blocks of 150?×?130?×?9?mm3 were prepared and divided into four groups of control samples, nano-silver-, Borax-, and Celcune-impregnated specimens. A special apparatus was designed, and six fire-retarding properties of each specimen were measured, namely ignition, glowing, back-darkening and back-holing times, as well as length and width of burning. Results showed that all three kinds of treatments had improving effects on the fire-retarding properties. Celcune showed the best improvement on glowing time. Cluster analysis showed direct relation between density and fire-retardant properties; also, fire-retarding behaviors of the four hardwoods were quite different to those of the one softwood. Furthermore, there are improvements in some fire-retarding properties of nano-silver-impregnated specimens that are in connection with its heat-transfer property.     

Effect of Nutrient Solution PH on the Vegetative and Reproductive Growth and Physiological Characteristics of Rose Cv. ‘Grand Gala’ in Hydroponic System

The rose plants were cultivated in the pots containing 75% cocopeat+ 25% perlite and were nourished with ½ Hoagland nutrient solutions with pH of 4.5, 5.5, 6.5, and 8. The results revealed that plant fresh and dry weight, flower bud, and stem diameter increased with the increase in pH from 4.5 to 6.5, but then started to decrease with further increase in pH up to 8. The highest number of buds, chlorophyll content, F_v/F_m, performance index (PI), and leaf phosphorus, iron, manganese, and copper concentrations were obtained at pH 6.5 and the lowest one at pH 8. The highest and lowest zinc concentration was observed at pH 6.5 and 4.5, respectively. According to the results, pH 6.5 was evaluated as optimum for hydroponic culture of rose, which resulted in the higher uptake of elements and better vegetative and reproductive growth and higher quality of rose plants.     

Effect of Ammonium:Nitrate Ratios in the Response of Strawberry to Alkalinity in Hydroponics

Strawberry (Fragaria ananasa) plants were grown in hydroponics in a greenhouse, where the supply of different ammonium (NH₄⁺): nitrate (NO₃^?) ratios, was investigated to reduce the negative effect of alkalinity in nutrient solutions. The experiment was arranged in factorial combination with two factors, NH₄⁺:NO₃^? ratios (0%:100%, 25%:75%, 50%:50%, 75%:25%, and 100%:0%) and bicarbonate (0, 25, 50, and 75 mM; NaHCO₃). Plants treated with increasing bicarbonate concentrations exhibited significantly inhibited growth. In plants treated with NH₄⁺ and NO₃^? simultaneously, there was a counteraction of the bicarbonate-induced growth suppression. Sole NO₃^? application in the presence of high bicarbonate resulted in poor growth and plant death due to high alkalinity. The adverse effect of alkalinity on SPAD values and maximal quantum yield of PSII photochemistry (F_v/F_m) alleviated with increasing proportion of NH₄⁺ in nutrient solutions. The results showed that fruit numbers and yield of strawberry increased with elevation of NH₄⁺ in nutrient solutions.     

Energetic radiation produced during rocket-triggered lightning

Using a NaI(Tl) scintillation detector designed to operate in electrically noisy environments, we observed intense bursts of energetic radiation (> 10 kiloelectron volts) during the dart leader phase of rocket-triggered lightning, just before and possibly at the very start of 31 out of the 37 return strokes measured. The bursts had typical durations of less than 100 microseconds and deposited many tens of megaelectron volts into the detector. These results provide strong evidence that the production of runaway electrons is an important process during lightning.