The effects of exchangeable sodium percentage (ESP) and electrolyte concentration (C) on the relative hydraulic conductivity (RHC) and dispersion index (DI) of two soil samples selected from a Vertisol and an Aridisol were investigated in the laboratory. For the two soil samples RHC increased whereas DI decreased as ESP decreased and C increased. DI was found to account for over 80% (r0.001 = 0.62) of the variability of RHC of a salt-affected sample. DI could thus be used for predicting RHC. ESP—threshold concentration relationships indicated that a saturated gypsum solution could be initially effective in a disturbed soil column taken from a Gezira Vertisol of ESP > 9 and a montmorillonitic Aridisol of ESP > 16. Dispersion indices that can be used as a guide for improving irrigation water quality were suggested. These laboratory findings are thought to be useful in planning field investigations regarding the reclamation of the soils that were sampled. 相似文献
Grape seeds contain high levels of phytochemicals, that have been associated with a reduced risk of chronic diseases. In this study, the polyphenolic content, antioxidant capacity and antioxidant enzymes activity in the seeds of 20 wild grape accessions native to Iran were investigated. The antioxidant capacity of the wild grape seeds was evaluated by DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and Fe2+ chelating. Total phenolic (TP), total flavanoid (TF) and total proanthocyanidin (TPA) content ranged from 44.86 to 155.1, 23.21 to 131.91, and 10.11 to 26.10?mg/g seed, respectively. All seed extracts showed remarkable DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and Fe2+ chelating ranging from 27.34 to 78.57%, 198.83 to 590.86?µmol TE/g and 23.12 to 62.98%, respectively. Grape seed extracts had the greatest antioxidant enzymes activity guaiacol peroxidase (G-POD), superoxide dismutase (SOD), and catalase (CAT). Based on HPLC analyses, catechin was the most abundant compound identified (25.89?mg/g) for all accessions. Additionally, significant correlations among different antioxidant assays were found. With the high amount of total phenolic content, antioxidant capacity and antioxidant enzymes activity, seeds of P6, P7 and P8 accessions might be evaluated as a nutritional supplement. 相似文献
Heavy metals’ contamination of soil is a serious concern as far as public health and environmental protection are concerned. As a result of their persistent and toxic properties, heavy metals need to be removed from contaminated environments using an efficient technology. This study is aimed to determine the heavy metals’ (Ni, Pb, and Zn) bioremoval capacity of consortia of filamentous fungi from landfill leachate-contaminated soil.
Materials and methods
Three different groups of consortia of fungi, namely all isolated fungi, Ascomycota, and Basidiomycota, were employed for the bioremediation of the contaminated soil. A total of thirteen fungal species were used to make up the three consortia. The setup was kept for 100 days during which regular watering was carried out. Soil subsamples were collected at day 20, day 60, and 100 for monitoring of heavy metal concentration, fungal growth, and other physicochemical parameters.
Results and discussion
Highest tolerance index of 1.0 was recorded towards Ni and Zn concentrations. The maximum metal bioremoval efficiency was observed for soil bioaugmented with the all isolated fungi for Ni and Pb with the removal efficiencies as 52% and 44% respectively. However, 36% was realized as the maximum removal for Zn, and was for Ascomycota consortium-treated soil. The order for the heavy metal removal for Ni and Pb is all isolated fungi > Basidiomycota?>?Ascomycota, while for Zn is Basidiomycota?>?all isolated fungi > Ascomycota. Spectra analysis revealed the presence of peaks (1485–1445 cm?1) only in the consortia-treated soil which corresponded to the bending of the C–H bond which signifies the presence of methylene group.
Conclusions
Soil treated using bioaugmentation had the best heavy metal removal as compared to that of the control. This suggests the contribution of fungal bioaugmentation in the decontamination of heavy metal–contaminated soil.
Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production. 相似文献
This study investigates the effects of inclusion of low levels of dietary short chain fructooligosacchairde (sc‐FOS) on physiological response and intestinal microbiota of carp (Cyprinus carpio) larvae. After acclimation, fish (550 ± 20 mg) were allocated into nine tanks (40 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5% and 1% sc‐FOS for 7 weeks. At the end of the experiment, the growth performance parameters (final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR) and condition factor (CF), survival rate as well as digestive enzyme activities (amylase, lipase and protease), total viable counts of heterotrophic aerobic bacteria (TVC) and lactic acid bacteria (LAB) level in intestinal microbiota were measured. Our results revealed no significant (P > 0.05) effects of sc‐FOS on growth performance and TVC when compared with the control group. However, administration of low levels of dietary sc‐FOS significantly increased digestive enzyme activities (lipase and amylase) and LAB levels (P < 0.05). Also, survival rate was significantly elevated in sc‐FOS fed carp. These results revealed that administration of low levels of sc‐FOS can be considered as a beneficial dietary supplement for larval stage of common carp. 相似文献
Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the focused identification of germplasm strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species. 相似文献