Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution

 A ¹⁵N isotope dilution technique was applied to quantify the extent of N₂ fixation in lentil (Lens culinaris Medik.) cultivars as influenced by Rhizobium leguminosarum bv. viciae strains in a field experiment in Pakistan. The experiment was conducted on a soil with a very small indigenous rhizobial population and where N was a limiting factor for crop production. Significant variations in number of nodules, dry weight of nodules, biomass yield, grain yield, total N yield, proportion of plant N derived from N₂ fixation (P_fix) and amount of N derived from the atmosphere (N_dfa) were observed among combined treatments of four rhizobial strains and six lentil varieties. In a field previously labelled with ¹⁵N, to which a basal dose of 75 kg P₂O₅ ha^–1 was applied as single super phosphate, N_dfa ranged from 15 to 24 kg N ha^–1 when calculated according to rhizobial strain and from 4 to 38 kg N ha^–1 when calculated according to lentil variety. Lc 26 was the most effective strain and fixed 243% more N than the indigenous population in the uninoculated control. In treatments with the lentil variety PL-406, N_dfa was 38 kg N ha^–1, which was 850% higher than with the lentil variety Precoz/F6-20-1×M-85. Generally, the varieties with greater P_fix produced a higher dry matter yield. Received: 26 May 1999     

The feasibility of using an industrial sewage sludge produce in Pakistan as agricultural fertilizer used for cultivation of Sorghum bicolor L

Abstract

In the present study we evaluate the feasibility of using untreated industrial sewage sludge by liming before use as a fertilizer, produced in Pakistan. In a pots experiment, limed industrial sewage sludge (LSW) and non-limed sewage sludge (NLSW), were amended with soil separately and grown sorghum. After maturity, the sorghum grains were analysed for total contents of potentially toxic metals (TPTM), As, Cd, Cr, Cu, Ni, Pb and Zn. The proportion of different mobility fractions of each element in LWS and NLSW, a modified BCR sequential extraction procedure (Community Bureau of Reference) and single extractions with mild extractants (deionized water and CaCl₂) were used. In LSW, the availability of most of the elements under study was reduced, probably due to the increased pH of soil, while this was the reverse in the cases of Cd and Cu, their mobility was slightly increased by lime-treated sludge. The sorghum grains grown in LSW have low level As, Cr, Ni, Pb and Zn as compared to grains grown in NLSW, except Cu and Cd, which, however, never exceeded legal limits. Thus the research showed that liming, by augmenting soil alkalinity, allows a safe agricultural use even of industrial sludge, which is environmentally hazardous for its great content of heavy metals.     

Assessment of the resilience and resistance of remediated soils using denitrification as model process

Purpose

Soil contamination by pollutants is increasing, urging for remediation strategies but little is known about the functional sustainability of these strategies.

Materials and methods

We assessed the resistance and resistance of a microbial respiratory process, denitrification, to two different levels of heat-drought disturbances among (1) thermally treated industrial soil, (2) constructed Technosol made of thermally treated soil, compost, and paper by products, and (3) an arable soil.

Results and discussion

We showed that thermal remediation lead to low resistance and resilience after disturbances. However, addition of compost and paper mill sludge improved the stability.

Conclusions

This work underlines the relevance of resistance and resilience ecological concepts for assessing remediation strategies.     

Spatio-temporal variability of drought characteristics across Pakistan

Climate change in Pakistan has a great impact on the spatial and temporal variation of precipitation and ultimately alters the frequency and duration of droughts. In this study, spatial and temporal trend analyses of precipitation and droughts were observed at 58 meteorological stations across Pakistan from 1981 to 2018. The existing trend analysis methods were evaluated to address the issue of serial correlation in the climatic data. Results of precipitation analysis showed significant decreasing trends in winter (November, December) and significant increasing trends were observed in summer (June and September) at a confidence level of 95 percent. The magnitude of the precipitation trends showed the highest variation during summer season and the least variation in winter season. Rotated Principal Component (RPC) analysis showed the severe droughts (high positive loading) in southeastern side (Sindh province) of Pakistan due to lack of summer rains. Furthermore, variance correction approaches are identified as the most suitable in coping with the effect of serial correlation. The highest drought frequencies were observed in the southern areas of Pakistan and the drought events are expected to occur more frequently in the late winter, early spring, and early autumn, while droughts were expected to occur least frequently in summer.

     

Efficacy and persistence of native entomopathogenic nematodes against Capnodis tenebrionis in peach (Prunus persica) orchard in Turkey

Phytoparasitica - Entomopathogenic nematodes (EPN) have an important potential in the biological control of agricultural soil pests. Efficacy and persistence of native EPN species from Turkey were...     

Validation of molecular markers for resistance among Pakistani chickpea germplasm to races of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">ciceris</Emphasis>

DNA markers in chickpea, targetting resistance genes for different races of Fusarium oxysporum f.sp. ciceris (Foc), have been identified in chickpea, but validation of these markers is essential for effective use in resistance breeding. In view of this, different simple sequence repeats (SSR) markers were analysed in Pakistani germplasm including induced mutants and some local lines. Most of the SSR markers showed good correlation with phenotypic evaluation of genotypes to different races of Foc and may be used effectively in resistance breeding, except those markers for race 3. Markers for race 3 showed deviations from phenotypic data and the reason might be that race 3 is actually Fusarium proliferatum as reported recently and resistance to this race might involve some other major resistance genes. Poor correlation of markers with foc-3 on LG2 in our study and a recent report of independent segregation of foc-2 and foc-3 in near isogenic lines suggested that linkage distances among different resistance genes need further investigation. Moreover three Pakistani mutant lines (97477, CM444/92 and CM368/93) depicted high levels of resistance to Foc races and can be deployed as a valuable source in resistance breeding programmes.     

Growth and survival of cowpea bradyrhizobia in various carrier materials

Summary Pakistan does not yet have the technology for commercial production ofRhizobium andBradyrhizobium inoculum. Therefore, investigations were undertaken to evaluate the suitability of different materials like compost, sawdust, rice husks, sugar cane, filter mud, and peat asBradyrhizobium carriers. The growth and survival of bradyrhizobia (strain TAL 441 of the cowpea type) was studied in sterilized and unsterilized carriers mixed with loam and enriched with lucerne meal and sucrose. Three different sterilization methods (autoclaving, gamma irradiation, and dry heating of the carriers) were used. The growth and survival of bradyrhizobia in the inoculants were studied at two different storage temperatures, 4° and 20°C. After 2–21 months of inoculation, maximum survival of rhizobia (7.6 × 10⁹ cells g^–1) was observed in autoclaved filter mud containing loam-lucerne meal and sucrose. The survival of rhizobia in autoclaved peat was 3.4x 109 cells g-t. The maximum viable number of rhizobia per seed of mungbean (Vigna radiata) was 7.7 × 10⁸ in gamma-irradiated compost and least (1 × 10⁷ cells seed^–1) in rice husks.     

Mapping and assessing water use in a Central Asian irrigation system by utilizing MODIS remote sensing products

Spatial and temporal patterns of water depletion in the irrigated land of Khorezm, a region located in Central Asia in the lower floodplains of the Amu Darya River, were mapped and monitored by means of MODIS land products. Land cover and land use were classified by using a recursive partitioning and regression tree with 250 m MODIS Normalized Difference Vegetation Index (NDVI) time series. Seasonal actual evapotranspiration (ET_act) was obtained by applying the Surface Energy Balance Algorithm for Land (SEBAL) to 1 km daily MODIS data. Elements of the SEBAL based METRIC model (Mapping Evapotranspiration at high Resolution and with Internalized Calibration) were adopted and modified. The upstream–downstream difference in irrigation was reflected by analyzing agricultural land use and amounts of depleted water (ET_act) using Geographical Information Systems (GIS). The validity of the MODIS albedo and emissivity used for modeling ET_act was assessed with data extracted from literature. The r ² value of 0.6 indicated a moderate but significant association between ET_act and class-A-pan evaporation. Deviations of ET_act from the 10-day reference evapotranspiration of wheat and cotton were found to be explainable. In Khorezm, seasonal maximum values superior to 1,200 and 1,000 mm ET_act were estimated for rice and cotton fields, respectively. Spatio-temporal comparisons of agricultural land use with seasonal ET_act disclosed unequal water consumption in Khorezm. Seasonal ET_act on agricultural land decreased with increasing distance to the water intake points of the irrigation system (972–712 mm). Free MODIS data provided reliable, exhaustive, and consistent information on water use relevant for decision support in Central Asian water management.     

Chilling Tolerance in Hybrid Maize Induced by Seed Priming with Salicylic Acid

The optimum temperature for maize germination is between 25 and 28 °C. Poor and erratic germination at suboptimal temperature is the most important hindrance in its early sowing. This study was conducted to induce chilling tolerance in hybrid maize (Zea mays L.) by seed priming with salicylic acid (SA) and to unravel the background biochemical basis. For seed priming, maize hybrid (Hycorn 8288) seeds were soaked in 50, 100 and 150 ppm (mg l^?1) aerated solutions of SA for 24 h and were dried back. Treated and untreated seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Performance of maize seedlings was hampered under chilling stress. But seed priming with SA improved the seedling emergence, root and shoot length, seedling fresh and dry weights, and leaf and root score considerably compared with control both at optimal and chilling temperatures. However, priming in 50 mg l^?1 SA solution was more effective, followed by priming in 100 mg l^?1 SA solution. Seed priming with SA improved the chilling tolerance in hybrid maize mainly by the activation of antioxidants (including catalase, superoxide dismutase and ascorbate peroxidase). Moreover, maintenance of high tissue water contents and reduced membrane permeability also contributed towards chilling tolerance.