Effect of the Immunity Efficacy of IBD Immune Complex Vaccine on ND La Sota Vaccine

1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens.     

吉林省降雪初终日时空变化特征

利用吉林省1961-2015年逐日降水和天气现象资料,对降雪初、终日的时空变化特征进行了分析。结果表明:吉林省降雪平均日期始于10月22日,止于次年4月18日。降雪初、终日存在空间差异,东部山区开始早结束晚,西部平原开始晚结束早。降雪初、终日的空间第一模态为全省一致的偏早(晚)型,初日第二空间模态为南北反向型,终日第二向量场为东西反向型。初日集中在10、11月,以10月中旬出现概率最大,终日集中在3-5月,以4月中旬出现概率最大。初日存在8 a强显著周期,终日存在5 a强显著周期。降雪初、终日与大气环流有直接关系,初日推迟年西太平洋副高面积偏小、偏北,提前年副高面积偏大、偏南;终日推迟年副高面积偏小、偏南,提前年副高面积偏大、偏北。降雪初、终日还与地理环境有关,降雪初日随海拔的降低呈推迟趋势,终日则随着纬度升高和海拔降低呈提前趋势。     

国家种质广州甘薯圃病毒种类鉴定及分析

为明确引起国家种质广州甘薯资源圃中病毒病的病毒种类及优势种,为甘薯种质安全保存提供支持,2017年从甘薯资源圃中未脱毒更新的盆栽苗和大田苗中采集155份具有不同病毒病症状的甘薯资源样品,利用PCR和RT-PCR检测技术对这些样品进行了17种病毒的分子检测.155份样品均有病毒检出,包括甘薯羽状斑驳病毒Sweet pot...     

Effect of heat shock precondition on reperfusion arrhythmia in rats

AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na⁺ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na⁺ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity.     

Effects of protein kinase C on modulation of vascular endothelium permeability

Protein kinase C pathway is an important intracellular signal transduction pathway. A growing number of evidences showes that activation of PKC influences endothelial cell permeability. In this review, we briefly summarize the effects and regulating pathways of protein kinase C in modulation of vascular endothelium permeability.     

Effects of riboflavin and ascorbic acid on apoptosis and proliferative inhibition of mouse thymocytes induced by deoxynivalenol in vivo

AIM: To explore the effects of riboflavin and ascorbic acid on the apoptosis induced by deoxynivalenol(DON) in mouse thymocytes. METHODS: The effects of riboflavin and ascorbic acid on the apoptosis and proliferation inhibition of thymocytes induced by DON in KM mice were studied with animal experiment, DNA agarose gel electrophoresis and flow cytometric DNA content analysis. RESULTS: Apoptosis rate of thymocytes in DON (4 mg/kg) treated group was13.73%±15.3% The percentages of apoptosis in riboflavin (1.25 mg/kg-10mg/kg) and ascorbic acid (25 mg/kg-100mg/kg) pretreated thymocytes groups were significantly lower than that in DON group (P <0.05). The result of DNA agarose gel electrophoresis showed that the characteristic ladder pattern of apoptosis was found in DON-treated thymocytes, but not in control and riboflavin pretreatment and ascorbic acid pretreatment groups The significant differences in proliferation index were not found among DON-treated thymocytes and riboflavin and ascorbic acid-pretreated thymocytes CONCLUSION: Pretreatment with riboflavin and ascorbic acid inhibit apoptosis of mouse thymocytes induced by DON in certain extent and have no effect on proliferation inhibition by DON.     

中早熟西瓜新品种科丰16号的选育

科丰16号是以自交系A45为母本,以自交系花大板为父本育成的西瓜一代杂种。中早熟,全生育期95 d左右,果实发育期30 d左右,主蔓第7~8节着生第1雌花,以后每5~7节现1雌花;植株长势强,坐瓜整齐,果实圆形,果皮绿色覆墨绿色条带,外形美观;瓤肉红色,口感好,瓤质沙脆,中心可溶性固形物含量12%左右;单瓜质量7 kg左右,667 m~2产量4 500 kg左右;抗病性强、适应性广,适宜陕西及相同生态区早春保护地及露地栽培。     

蔬菜高效水肥一体化灌溉技术的实践与发展建议

水肥管理是直接影响蔬菜稳产高产和品质提升的关键因素。总结了我国蔬菜高效灌溉的4种主要模式,介绍了不同种类蔬菜的高效灌溉模式,分析了我国蔬菜高效水肥一体化灌溉技术存在的问题,并提出了发展建议。     

意蜂来源的微孢子虫对不同日龄中蜂成年工蜂的侵染性

为探明意蜂来源的微孢子虫对不同日龄中蜂成年工蜂的交叉侵染性,将纯化自意蜂的微孢子虫分别接种无微孢子虫感染的2日、5日、8日、11日、14日龄的中蜂工蜂,当每只蜂经口接种5.12×104个孢子,在30±1℃,45%～60%RH,黑暗环境中饲养12天后,2～14日龄的中蜂工蜂的感染率为46.7%～72.6%,平均感染率为65.8%;其中2日龄的感染率显著(P<0.05)低于5～14日龄的感染率。