加强综合管理 提高仔兔成活率

仔兔的适应能力和抵抗力都较差,饲养管理稍有不慎,都会造成仔兔的死亡,从而降低养兔的经济效益。综述了母兔产前准备及产后的管理,认为加强母兔的饲养管理是养好仔兔的基础以及提高仔兔成活率的保障。     

microRNA在骨骼肌发育中的功能及其表达的营养调控

microRNA( miRNA)是真核生物中一类参与调控基因表达的非编码小分子RNA,它通过翻译抑制和使靶mRNA降解,在基因表达中起着负调控作用,与骨骼肌发育、疾病等密切相关.营养可以调控miRNA及其靶基因的表达,这将为更深层次诠释营养素的作用机理提供新的研究方向.本文综述了miRNA的结构和作用机制、骨骼肌miR...     

中早熟西瓜新品种科丰16号的选育

科丰16号是以自交系A45为母本,以自交系花大板为父本育成的西瓜一代杂种。中早熟,全生育期95 d左右,果实发育期30 d左右,主蔓第7~8节着生第1雌花,以后每5~7节现1雌花;植株长势强,坐瓜整齐,果实圆形,果皮绿色覆墨绿色条带,外形美观;瓤肉红色,口感好,瓤质沙脆,中心可溶性固形物含量12%左右;单瓜质量7 kg左右,667 m~2产量4 500 kg左右;抗病性强、适应性广,适宜陕西及相同生态区早春保护地及露地栽培。     

Effect of eleutheroside B/E on proliferation and expression of PPARγ and TGF-β1 in HBZY-1 cells cultured with high glucose

AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression.     

梅州金柚产业分析

金柚产业是梅州最具有区域特色的支柱产业,本文从梅州市金柚的产业现状、发展优势、存在问题作分析,在立足品牌促产业转型升级上进行探索,为做大做强金柚产业提供参考。     

生根处理和扦插时间对杉木容器扦插育苗效果的影响

以通过审定的广西杉木(Cunninghamia lanceolata)良种为扦插材料,探讨不同的生根处理方法和扦插月份对扦插生根效果及成活率的影响。结果表明:使用生根剂处理的穗条,其生根率、成活率、根系数量及整齐度均极显著高于对照,ABT1和IBA处理的穗条根系萌发最快,根系萌发时间均为22 d,比对照提前13 d;ABT1处理的穗条生根率(97.8%)和平均根系数(4.2)最高,整齐度最好,其次为IBA;ABT1、IBA和NAA处理的穗条成活率分别为97.8%、97.2%和93.1%,对照成活率为81.5%;杉木扦插苗培育的最佳扦插时间为3~5月。     

数控加工一体化教学实践与探索

文章介绍了一种全新的教学模式——数控加工一体化教学模式,从他的内涵出发,分析了其在实践中所具有的优势,发现其中的不足,并提出一些参考性意见,希望能为数控人才的培养提供参考,促进数控机床加工技术的发展进步。     

Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

福州市周边蛋鸡场禽白血病亚群的血清流行病学检测

应用ELISA方法对分别采集自福州市周边30个蛋鸡场的597羽海兰蛋鸡、512羽罗曼蛋鸡、486羽伊莎蛋鸡和553羽特佳蛋鸡共2148羽的血样进行禽白血病病毒(ALV)亚群ALV-A/B和ALV-J抗体检测.结果表明:样品ALV-A/B抗体阳性率由高至低依次为:特佳蛋鸡群11.39%(63/553)、罗曼蛋鸡群11.32%(58/512)、海兰蛋鸡群8.54%(51/597)和伊莎蛋鸡群7.61%(37/486);样品ALV-J抗体阳性率由高至低依次为:特佳蛋鸡群5.24%(29/553)、罗曼蛋鸡群4.10%(21/512)、伊莎蛋鸡群3.51%(13/486)和海兰蛋鸡群3.18%(19/597);而样品ALV-A/B抗体总阳性率为9.73%(209/2148),高于ALV-J抗体总阳性率3.82%(82/2148),其中有0.70%(15/2148)的ALV-A/B和ALV-J抗体双阳性样品;蛋鸡场ALV-A/B抗体阳性率为70.00%(21/30),高于ALV-J抗体阳性率36.7%(11/30),其中有16.7%(5/30)的ALVA/B和ALV-J抗体双阳性样品.结论:福州市周边蛋鸡场不同品系和不同鸡群间存在不同程度的ALV流行,相比ALV-J,ALV-A/B流行更普遍,且存在ALV-A/B和ALV-J抗体双阳性样品,应予重视.     

茶园间作芳香植物罗勒和紫苏对茶园生态系统影响的研究

通过茶园间作芳香植物研究其对茶园害虫和天敌种群数量、土壤养分状况、茶树生长及茶叶生化成分等的影响,分析间作芳香植物对幼龄茶园的生态综合调控效果。幼龄茶园间种芳香植物能减少小贯小绿叶蝉和绿盲蝽的数量,增加茶园中瓢虫、草蛉、寄生蜂和蜘蛛等天敌的数量;芳香植物间作区土壤中铵态氮、有效磷和速效钾的含量高于绿肥间作区和对照;茶园间作芳香植物能在一定程度上促进茶树生长、培养幼龄茶园的树势和树冠、增加茶叶产量。茶园间作芳香植物能减少茶叶中茶多酚和咖啡碱含量,提高可溶性糖和儿茶素含量,改善茶叶品质。可见茶园间作芳香植物是一种具有较好生态效益的茶园栽培管理模式,能促进生态茶园的发展。