小鹅瘟PCR诊断方法的建立和初步应用

根据发表的鹅细小病毒B株VP3基因序列 ,设计合成了 1对寡聚核苷酸引物。以国内分离的小鹅瘟病毒为摸板 ,筛选了 (PCR)最佳反应条件 ,并进行了敏感性、特异性和初步应用试验。结果表明 ,在 4 0 μL体积中 ,PCR最佳系统组成为 2uTaq酶、0 5mol/LdNTP和 2 0 pmol引物 ;最佳反应参数为 96℃变性 4 0s ,5 4℃退火 1min ,72℃延伸 1min ,30个循环。本方法可检出 2 5× 10 -1 5EID50 小鹅瘟病毒 ,并且仅能从小鹅瘟病毒的鹅胚培养物中扩增出与设计值大小相同的 375bp核苷酸片断 ,对照病毒扩增结果为阴性。通过对 6份临床病料的检查 ,2份呈阳性 ,其中 1份来自有典型小鹅瘟症状的雏鹅 ,1份来自无典型小鹅瘟症状的雏鹅。由此说明 ,本试验所建立的诊断小鹅瘟的PCR方法具有潜在的临床应用价值     

苹果渣饲喂泌乳牛的试验效果

苹果渣是生产苹果汁的下脚料,营养丰富,适口性好,近几年,随着苹果加工厂的增加,我市许多养奶牛户利用苹果渣下脚料饲喂奶牛,不仅解决了苹果渣造成的环境污染问题,而且提高了奶牛产量,降低了生产成本,增加了奶牛养殖业效益。为进一步验证和推广该技术,笔者于1999年在我场进行了利用苹果渣饲喂泌乳牛的对比试验,取得了理想效果。     

通过病毒分离鉴定和基因检测首次发现虎流感

应用F81猫肾传代细胞，从高热、拒食和间有神经症状的死亡率病科中分离获得1株病毒，经形态学、理化学、生物学和血清学系统鉴定，证明为流感病毒。采用流感病毒核蛋白基因引物，对分离病毒及该虎病科进行RT－PCR扩增和序列分析，结果从分离病毒和虎病科中均扩增出与理论值大小相符的464bp基因片段；其序列与A、B、C3型流感病毒相比较，同源性分别为84．9％、35．0％、24．8％。由此说明，所分离病毒为A型流感病毒，命名为/蘧／哈尔滨（中国）／01／2002。用此分离病毒静脉接种于3月龄家猫3号，均出现与病虎相似的临床症状，其中1号耐过，2只死亡。死亡猫剖检变化与死虎相似，主要是呈肺炎病变，并可从病科中回收到所接种病毒。从此分离病毒为HA抗原，进行虎与猫血清的HI抗体检测，结果康复虎血清比其病初血清、康复猫血清比其接种前血清HI抗体均增高4倍以上，表明该病毒具有致病性，是引起该虎与试验猫发病甚至死亡的病原。     

In vitro viability of cryopreserved equine embryos following different freezing protocols.

The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos.     

春制越年家蚕卵乙胚子冷藏方法研究

研究结果表明，春制越年家蚕卵丝乙胚子冷藏处理后，其实用孵化率〉９２．３％蚁蚕绝食致半数时数≥１１０ｈ，不良卵发生率〈１０％，发病率〈８．６％，茧质优良，产量平稳，在有效冷藏期限内随时出库催青饲育均安全可靠。     

Physiological and morphological changes in bovine mammary glands following intramammary infusion of recombinant interferon-gamma.

Eleven lactating dairy cows were used to evaluate the response of bovine mammary glands to increasing doses of recombinant bovine interferon (rBoIFN)-gamma. Right front and rear quarters were intramammarily infused with eight different doses (10(2) U to 2 x 10(8) U/quarter) of rBoIFN-gamma; each dose was tested in at least two quarters. Left udder halves served as within animal controls in which quarters were injected with a saline placebo or were not infused at all. Milk secretion samples for compositional analysis were collected from each quarter prior to infusion and at 6, 24, 36 and 48 h following infusion. Animals were slaughtered immediately following the 48 h sampling period and mammary tissue was obtained for morphometric analyses. Milk composition was similar between control quarters and those quarters infused with up to 10(5) U of rBo-IFN-gamma during the entire sampling period. Quarters infused with 10(6) U and 10(7) U of rBoIFN-gamma had higher milk somatic cell counts (SCC) following treatment compared with preinfusion values. Changes in the composition of mammary secretion were most dramatic in quarters infused with greater than or equal to 10(8) U of rBoIFN-gamma as indicated by the significant increase in SCC and milk pH with a concomitant decrease in lactose concentration when compared with pre-infusion values or with control quarters. Morphometric analysis of tissue demonstrated an increase in stroma, a decrease in luminal area, and a marked increase in the number of infiltrating leukocytes in those quarters infused with the higher doses of rBoIFN-gamma.(ABSTRACT TRUNCATED AT 250 WORDS)