粗梗稠李中花色苷的提取及抗氧化和抑菌活性研究

以粗梗稠李成熟果实为试材,采用超声辅助法提取花色苷,通过单因素正交实验优化花色苷的提取工艺,并测定其抗氧化和抑菌活性,以期为粗梗稠李功能农产品的开发提供参考依据.结果 表明:花色苷的最佳提取条件为乙醇体积分数50％、料液比为1∶ 60g·mL-1、pH 1.0、超声时间50 min、提取温度40℃,该条件下,花色苷的平均提取量为0.632 mg·g-1;粗梗稠李花色苷对·OH、DPPH·、ABTS+·、O2-·均有较好的清除能力,高浓度时对FE2+有较好的螯合能力;对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌有较强的抑菌效果,其最低抑菌浓度(MIC)分别为12.49、12.49 mg·L-1和6.25 mg·L-1.     

临猗县冬枣园土壤肥力综合评价

在临猗县抽样分析了24个冬枣园土壤有机质、速效氮、磷、钾及微量元素含量,了解临猗县冬枣种植区土壤肥力状况及科学评价土壤环境质量,以期为提高土壤管理水平提供参考依据.结果表明:土壤有机质含量范围为0.29％～1.10％,有效磷含量范围为1.09～17.55 mg·kg-1,2个指标在绝大多数地块含量极低;碱解氮含量范围为21.70～260.05 mg·kg-1,含量中等以上占比62.5％.速效钾含量范围为63～307 mg·kg-1,含量中等以上占比70.83％;pH范围为8.11～8.97,显示土壤为碱性和强碱性;其它微量元素含量不平衡,有效铁、锰足够,交换性钙、交换性镁、有效硼含量丰富,有效锌含量极低.通过主成分分析,将24个样地分为3个肥力等级,一等肥力样地数量在24块样地中占比37.5％、二等占比8.3％、三等占比54.2％.通过用主观与客观赋值相结合的方法进行权重分析,该区肥力指标权重依次为有效磷、有机质、碱解氮、速效钾、pH、有效铁、交换性钙、有效硼、有效锰、有效锌、交换性镁.因此在生产中要根据土壤养分丰缺,除重视有机质和大量元素的补充外,还要有针对性的补充极缺的微量元素,以提高肥料利用率、减少浪费,避免过量施用化肥或盲目补充微量元素肥造成土壤质量下降和环境污染.     

用中医药博士论文引文分析中医药科研人员的外文文献需求

以中国中医科学院2007-2009年的164篇中医药博士学位论文的外文引文作为研究对象,采用文献计量学方法对其外文引文的类型、数量、期刊被引年代分布、学科专业分布、语种分布、外文期刊被引频次等指标进行了统计与分析,以此了解中医药科研人员对外文文献需求的特点和规律,为图书馆资源建设和用户服务提供指南和参考。     

镉与苄嘧磺隆复合污染对水稻细胞、DNA的毒害作用

以粤香占和丰美占两种水稻为指示植物，通过透射电镜、扫描电镜以及彗星实验，在根尖细胞超微结构和DNA分子水平上研究了重金属元素Cd、苄嘧磺隆（Bensulfuron Methyl，BSM）复合污染对水稻的毒理学机制。电镜分析结果表明BSM和Cd复合污染能够破坏水稻根尖的细胞结构，使表皮细胞破裂，核仁解体。对丰美占水稻进行彗星实验研究，并用CASP软件对图像进行分析。研究结果表明，随着Cd^2＋浓度的增加，DNA的迁移程度逐渐增加，也就意味着DNA的受损程度逐渐增加。当Cd浓度为10mg·L^-1时，0．1mg·L^-1的BSM会使OliveTM等参数降低，这说明0．1mg·L^-1的BSM能够在一定程度上缓解Cd对水稻叶片DNA的损伤。     

概念隐喻及其表达式的认知语境研究

认知语境是一种概念化了的知识结构状态,它对话语意义的理解具有重要意义。概念隐喻在认知语言学中被认为是一种认知机制或认知模式,它是对日常隐喻语言的概括和总结,它具有概括性、系统性和生成性等特点。隐喻映射是始源域和目标域之间一种本体对应的关系,而映射过程中认知语境对＂蕴涵＂选择的限定功能确证了概念隐喻及其表达式的生成及其理解过程是在认知语境的共同参与下完成的,同时认知语境对概念隐喻及其表达式的理解有一定的制约作用。     

小型太阳能直流水泵的性能测试研究

自行配置了一套小型太阳能直流水泵,该设备具有装置简单、经济成本低、稳定性好及实用等优点,并在昆明地区进行了性能测试研究。其测试结果表明:辐照度大于450W/m2时该设备运行良好,并且提水高度在0～30m时较适用。可用于阳光充足无电力地区的深井取水、农作物灌溉、及河流、水库、水渠等提水工程。     

益生酵母在滩羊日粮中适宜添加量的研究

在精粗比为3∶7的滩羊日粮中,添加不同剂量的益生酵母(0,0.1%,0.3%,0.5%和0.7%)进行了体外批次培养,并应用单项组合效应值(SFAEI)和多项组合效应指数(MFAEI)对中性洗涤纤维(NDF)含量、氨氮(NH3-N)浓度、菌体蛋白(BCP)浓度、pH、产气量和总挥发性脂肪酸(VFA)浓度进行评定。单项组合效应值评定结果表明:NDF含量以添加量为0.1%组高于其他添加组,NH3-N浓度以0.3%组较高,BCP浓度以0.1%和0.3%组较高,pH以0.3%和0.5%组较高,产气量和总VFA浓度均以0.3%和0.7%组较高。多项指标综合评定结果表明:在精粗比为3∶7的日粮条件下,益生酵母在滩羊日粮中的适宜添加量为0.3%。     

CD147 monoclonal antibody-mediated nanoparticles for gene therapy to target lung cancer cells

AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells.     

钙蛋白酶3(CAPN3)基因多态性与牛胴体性状的关联分析

为了探讨钙蛋白酶3(Calpain3,CAPN3)基因作为影响牛肉质性状候选基因的可能性,寻找与牛肉质性状相关的分子标记,对CAPN3基因的5′端区域进行SNPs检测,分析不同基因型在不同品种或者组合群体间的分布规律。利用测序和PCR-RFLP的方法进行SNPs检测和基因型分析,计算等位基因频率、各位点多态信息含量。结果发现,2546位处发生点突变C→T。各位点的3种基因型与牛生产性状的最小二乘分析结果表明,AB与BB基因型个体在胴体质量指标上存在显著差异(P0.05)。初步推断CAPN3基因可能是影响牛肉质性状潜在的主效基因或与主效基因连锁,并且这个位点具有成为分子标记的潜在可能。     

不同结构对囊素样肽的免疫活性影响

化学合成单拷贝（BS）、30拷贝串联（KG30）和30拷贝分支结构（MAP）3种不同结构的囊素样肽，通过体外外周血淋巴细胞刺激增殖试验、杂交瘤细胞抗体分泌刺激试验、E-玫瑰花环试验和体内小鼠腹腔巨噬细胞吞噬功能、新城疫疫苗免疫应答的测定，比较了其免疫增强活性。结果表明，BS和MAP的活性显著高于KG30，BS体外试验活性优于MAP，而MAP体内试验活性优于KS。结构对囊素的活性有显著影响，以分支结构的囊素增强免疫效果最好。