番茄的结构-功能模型Ⅱ：基于器官水平的功能模型与验证研究

建立了番茄动态生长的功能模型,从个体和器官的角度,研究生物量在植物体内的生产和分配规律、器官的动态生长规则。模型根据蒸腾作用计算物质的生产;根据节间、叶柄、叶片和果实等器官的汇强和扩展规律对物质进行分配;根据与物质量的动态关系计算各器官的几何尺寸;利用试验数据对模型参数进行估计。通过对参数进行比较和分析得出它们对各器官生长的影响程度和器官本身的扩展规律。并对模型输出与测量数据的拟合程度进行了残差分析,验证了模型的合理性和有效性。     

光强对紫罗勒花青素含量及光合特性的影响

研究了不同光强对紫罗勒花青素含量、气体交换、叶绿素荧光特性及反射光谱的影响.结果表明:强光下紫罗勒叶片较厚,而弱光下叶片较薄.与弱光下生长的紫罗勒相比,强光下单位叶面积叶绿素含量和花青素含量较高、叶片光补偿点和光饱和点升高、表观量子效率下降、净光合速率(Pn)明显提高、最大光化学效率(Fv/Fm)和光系统Ⅱ性能参数(P...     

甜菜nia基因的克隆及不同氮素形态诱导的差异表达

利用同源序列克隆方法从二倍体甜菜品种Ty7中获得氮素诱导nia基因片段,通过RACE技术克隆nia基因全长序列,该基因ORF长度2 718 bp,编码905个氨基酸,并已在GenBank上登录(EU163265),基因组中nia以低拷贝数存在。nia编码蛋白的等电点为6.12,推测分子量为102 kD,以NADH为电子供体。为揭示不同氮素形态和处理对甜菜nia基因表达的影响,采用半定量PCR方法检测不同氮素形态诱导nia基因mRNA的表达,同时测定酶活力。结果表明,当铵态氮诱导nia基因时,低浓度的铵离子能促进基因的表达,过高浓度的铵离子抑制基因的表达。当硝态氮诱导nia基因时,随处理浓度的增加,nia的表达加强,呈正相关关系。用30 mmol L^–1硝态氮诱导4 h后,nia基因表达达最高值,约在6 h后,表达明显下降。     

Analysis on the Moisture Migration of Subgrade Soil with Constant Temperature Condition

A moisture migration test for unsaturated clayey loess along Shenyang-Haerbin Express Railway for Passenger Line was carried out to investigate the water movement of soil in an open system with constant temperature condition.It is shown that moisture transfer is mainly caused by matrix potential and gravitational potential energy.The water content by volume gradually decreases from the bottom to the top of specimen.Over time the capillary water height increases by the power function and eventually reaches a stable value.The water flux decreases with time by the logarithm function.Based on the model test,finite element method is adopted to simulate the moisture transfer process in soil under isothermal condition.It is found that the numerical results are in good agreement with the experimental data.The model is thereby used to simulate the rise of groundwater in the subgrade soil.During the whole process of moisture movement,pore water pressure gradually decreases from the bottom to the top of sample.The rapid change of pore water pressure in the early process contrasts with slow change in the late process.The pore water pressure has a linear change along the specimen when the seepage reaches steady state.     

Analysis of embedded gas detection system based on nano-porphyrin arrays

In view of the necessity of gas detection in environment, an embedded gas detection system is designed based on the basic principles of chromatography change of chemical reaction between nano-porphyrin arrays and micro-gas, which takes ARM9 S3C2440A as the core and nano-porphyrin array as the sensors. The overall program, the circuit organization and control software of the main function module such as spectrum signal acquisition, processing and monitoring are presented. With energy conservation differential equation, the control model of temperature and flow rate in the sample chamber is established, which can precisely control the detection conditions of chemical sensors. Some typical trace gases such as ammonia, enanthaldehyde, cyclohexane and hexanal are tested by the system, and the results show that the system can achieve rapid and accurate detection of trace volatile gas.     

实时定量RT-PCR方法评价壬基酚的雌激素效应

壬基酚是1种环境雌激素物质,以血清卵黄蛋白原作为生物标记物检测壬基酚的最低雌激素效应浓度高于10μg/L.利用亚成体青鱼进行不同浓度的(1、10、50、100μg/L)壬基酚暴露实验,运用实时定量RT-PCR方法,从分子水平上,对青鱼VTG-Ⅰ、VTG-Ⅱ、CHG-H和CHG-L的基因表达进行了研究,并对低浓度壬基酚的环境雌激素效应进行了分析.结果表明:1μg/L浓度的壬基酚暴露组青鱼肝脏的VTG-Ⅰ、VTG-Ⅱ、CHG-H、CHG-L基因表达均被显著诱导,说明实时定量RT-PCR能够检测1μg/L壬基酚     

串联启动子调控下的t-PAcDNA乳腺定位表达

由牛β-Casein启动子与大鼠WAP启动子串联构建了t-PAcDNA乳腺定位表达载体pSVL-WAPβ-PA，将3种表达载体pSVL-βb-PA,pSVL-WAP-PA及pSVL-WAPβ-PA分别注入怀孕家兔乳腺导管中，溶圈试验结果显示，3种表达载体均能在家兔乳腺中表达，且以分娩后第4d的乳汁中表达水平最高，分别为390，440，450ug.L^-1，本试验为进一步研究t-PA基因乳腺定位表达调控和建立t-PA乳腺生物反应器奠定了基础。     

大力发展农牧业产业化,推进内蒙古城镇化建设

文章从农牧民收入及构成入手，通过分析研究自治区农牧业和农村牧区经济结构中存在的问题、发展农村牧区经济所具备的优势，探索发展农牧业产业化来推进自治区工业化、城镇化的进程途径．以此来带动农村牧区服务业和第三产业的发展，推动农牧业人口向非农产业转移，不断提高农牧业生产效率和扩大农牧民增收途径。     

豫西地区石粉矿物元素含量分析

对豫西地区石粉中矿物元素的含量进行检测,结果表明,石粉中含有丰富的钙和铁等元素。其中龙门山石粉中钙的含量为18.00%,铁含量为4 357.36 mg/kg;新安县石粉中钙的含量为32.77%,铁含量为5 398.06 mg/kg。2种石粉是补充禽用饲料中钙、铁等元素的良好来源。     

不结球白菜农艺性状的主成分分析及与黑斑病的通径分析

调查了34个不同生态型不结球白菜品种的生物学性状及黑斑病发病情况，并对其进行了主成分分析和通径分析。结果表明，叶柄宽度、厚度、重量及叶绿素含量、茎重、叶片数与病情指数的相关性达1％或5％的显著水平。不结球白菜的叶重、叶柄重、叶柄厚、根重、叶片数、叶柄上、中、下宽、类胡萝卜素对农艺性状的解释能力可达83．4％，反映了不结球白菜农艺性状的绝大部分信息。叶柄宽度、厚度、重量及叶大小和数量、叶绿素对病情指数的直接负向作用依次增大，它们的值越大，则病情指数越小，品种的抗病性越强。