火炬松芽苗移栽容器育苗技术

介绍了火炬松形态特征及生物学特性,并论述了其种子处理、苗床、育苗基质的准备,播种、芽苗移栽、幼苗的管理以及病虫害防治等技术措施。     

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrH9014 in Wheat Line H9014-14-4-6-1

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 (a wheat cultivar susceptible to all Pst races identified in China). The seedlings of the parents and F 1 plants, F2 , F3 and BC 1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection (MAS) in breeding programs for stripe rust.     

浅谈圆柱齿轮精度标准的发展变化

圆柱齿轮精度是影响齿轮使用寿命和性能的主要因素。圆柱齿轮精度标准也经历了几十年发展完善的过程。详细论述了圆柱齿轮精度标准的发展历程和新旧标准的变化,提醒广大企业和设计人员积极使用新标准,推广新标准。     

清代壮族边疆地区的林果生产研究

对清代壮族边疆地区的林果生产情况进行了分析,结果表明：该地区林果生产既有量的扩张,又有质的变化,市场化与近代化发展趋向明显。林果生产发展推动了壮族边疆地区乡村手工业的发展,加速了地区的开发进程。     

海南慢行旅游廊道空间体系的构建

以慢行旅游廊道空间体系为研究对象,以空间体系的单元模型构建为切入点,选取海南"大兴隆旅游区"作为其单元模型的规划实验,初步探索海南慢行旅游廊道系统空间体系的构建。可为海南建设国际旅游岛提供参考。     

肾型传染性支气管炎病毒M基因的遗传分析及其在杆状病毒表达系统中的表达

对肾型鸡传染性支气管炎病毒分离株Ck/Jiangsu/DS10/2008株(DS10株)膜蛋白(M)基因进行扩增、克隆和序列测定,并与GenBank中的其它25株参考株的M基因进行比对分析并构建进化树,研究其遗传进化关系。另将M基因克隆至杆状病毒表达系统pFastBac1载体并转染Sf9昆虫细胞进行表达,经间接免疫荧光鉴定,用琼扩试验验证表达产物的反应性,以研究M基因表达产物的生物学活性。测序结果表明DS10的M基因全长为678bp,编码225个氨基酸。遗传进化分析表明,DS10病毒与其它不同致病型的传染性支气管炎病毒共属同一大分支。间接免疫荧光检测表明,M蛋白在Sf9细胞中得到表达,琼扩试验验证表达产物具有良好的反应性,以上结果表明所表达的M蛋白具有良好的生物学活性。本研究表达的M蛋白可为其生物学活性研究及其诊断抗原、新型疫苗开发提供基础材料。     

TM600-2型离心式空气压缩机喘振故障分析及解决措施研究

离心式压缩机是生产过程中十分重要的设备,解决离心式压缩机运行的质量和效率具有重要意义。笔者通过对日常工作的总结,分析了TM600-2型离心式空气压缩机喘振故障原因,并提出了几点自己关于解决改故障的方案,实践证明很好地改善了喘振的问题。     

秸秆覆盖对附子氮代谢几种相关酶活性的影响

以江油附子主栽品种为材料,采用随机区组设计,研究了稻草覆盖方式(整草覆盖、切割与粉碎覆盖)和数量对附子硝酸还原酶(NR)、谷草转氨酶(ALT/GOT)和谷丙转氨酶(AST/GPT)等几种氮代谢相关酶活性的影响.结果表明:各覆盖处理的酶活性水平均高于对照组,即秸秆覆盖处理均有效益；不同的秸秆覆盖量间差异性显著,覆盖量为1 kg/m的处理酶活性水平高于覆盖量为0.5 kg/m2和1.5 kg/m2的处理；虽然切割覆盖的酶活性水平高于整草覆盖和粉碎覆盖,但不同覆盖处理间差异性并不显著；切割覆盖1 kg/m2处理的NR、GOT和GPT酶活性水平虽均为最高,但整草覆盖1 kg/m更能够达到较高经济效益的栽培效果；3种酶之间存在较好的正相关性,具有一定的协同作用.     

辽宁省水稻产业发展现状及展望

水稻是辽宁省重要的粮食作物,水稻产业的健康发展对辽宁省粮食安全具有重要的作用。通过总结辽宁省水稻产业的发展现状,分析限制辽宁省水稻产业发展的主要因素,指出辽宁省水稻增产的途径,并展望了辽宁省水稻产业的发展方向。     

Cloning and analysis of cross-intron genomic gene encoding ACO in Paeonia suffruticosa Andr.

The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by ACC oxidase (ACO). In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO (FJ855434) has 1281 bases, containing four exons and three introns, encoding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.