Assessment of affecting factors in measuring activating platelets with the method of flow cytometry

AIM:To investigate the affecting factors of detecting platelet activation by flow cytometry (FCM).METHODS:Using decoagulant of natrium citricum, anticoagnlated peripheral venous bloods from 6 healthy donors were labeled with the method of three-colour immunofluorescence assay.Platelet activation markers fibrinogen receptor (Fib-R, PAC-1) and P-selectin (CD62P) were measured.In the same time, the reproducibility of FCM was assessed.RESULTS:The platelet activation markers PAC-1 and CD62P at each time point showed significant difference(P<0.05).The ratio was increased with time extending.The positive ratio of PAC-1 and CD62P immediately measured (within 10 min) was 2.7% and 3.5% less than those at time point of 30 min.The results were measured several times under different activation levels.The coefficient of variation was less than 5%.CONCLUSION:In room temperature and with decoagulant of natrium citricum, if the measurements of PAC-1 and CD62P are finished within 30 min after sampling, good reproducibility should be achieved.     

茄子的组织培养和植株再生体系研究

以2个茄子品种：红茄和紫长茄的子叶和下胚轴为外植体,探讨了不同生长调节物质对外植体分化的影响和不同品种、不同外植体的分化能力差异,并建立了茄子的高频率离体再生体系。结果表明：培养基MS＋2,4-D 1.0 mg/L＋6-BA 0.5 mg/L诱导愈伤组织效果最好;诱导不定芽方面,培养基MS＋4-PU 0.1 mg/L诱导效果最好;1/2MS培养基较MS培养基更适合植株生根。     

西瓜种质资源的研究进展

对国内外的西瓜种质资源的收集、鉴定利用和种质资源创新等方面进行阐述.总结国内外西瓜种质资源研究上取得的成就,并对我国西瓜种质资源存在的问题提出建议.     

闽南海区休渔前后张网作业渔获物组成比较分析

本文主要根据2008年闽南海区张网作业渔业资源监测资料,对休渔前后（4月和8月）张网作业渔获物组成进行比较分析。结果表明：2008年休渔前后都以捕经济种类幼鱼为主。4月带鱼、二长棘鲷、刺鲳、鲐鱼、中华管鞭虾等主要经济种类渔获重量占总渔获物重量69．6％,其中带鱼幼鱼数量最多,占总渔获数量的26．2％（每网渔获尾数744ind）,居第一位;且个体极小,基本上全为幼鱼,平均体重8．1g。8月带鱼、二长棘鲷、短尾大眼鲷、叫姑鱼、白姑鱼、须赤虾等主要经济种类渔获重量占总渔获物重量50．9％。带鱼数量明显减少（每网渔获尾数226ind）,但个体增大,肛长140mm以上占63．6％,平均体重54．5g,比4月增加46．4g。并结合2004、2007年有关资料,较为详细说明休渔前（4月）张网作业对近岸经济幼鱼、幼体损害比休渔结束后（8月）严重,通过休渔对带鱼等经济种类幼鱼资源起了一定保护作用。     

锦鲤保护性组织RNA提取及引物扩增研究

从养殖锦鲤各种组织中提取完整的RNA, 是分子生物学研究锦鲤应激表达、体色变异、生长发育等功能基因表达的关键实验。在不伤害养殖锦鲤个体健康的情况下, 取用鱼体的鳍条、鳞片、鳃丝、血液4种保护性组织,可以最大程度地减少对珍贵锦鲤个体的损伤, 并达到研究相关基因表达的目的。本研究利用TR izo l法提取RNA, 获得的各样品RNA条带完整、纯度高。结果表明, 4 种保护性组织提取的RNA 经过逆转录反应, 得到了高质量的CDNA。内标基因( B- actin)、热激蛋白基因( hsp70)、金属硫蛋白基因(MT)扩增得到清晰的条带, 通过NCB I数据上的BLAST比对证明, 各序列的同源性在95%以上。利用本研究的方法, 可以充分保护养殖锦鲤个体的完好性, 用于进一步的分子生物学研究。     

抗草甘膦栽培大豆种质挖掘及抗性生理研究

利用田间试验结合生物化学分析研究了不同的栽培大豆品系对草甘膦的抗性,试验结果显示:在草甘膦有效剂量为0.31~0.92 kg·hm~(-2)时,不同栽培大豆品种对草甘膦抗性存在明显差异,在草甘膦剂量为0.92 kg·hm~(-2)时,石豆1号存活率为100%,表现了较高的抗性,8份材料的存活率为4%~48%,表现出一定的抗性;44份材料表现敏感,存活率为0。试验研究了抗性材料石豆1号、中抗材料Williams和敏感材料中黄35对草甘膦的生理生化反应的差异,结果表明:随着草甘膦处理剂量的增加和处理时间的延长,石豆1号莽草酸含量、叶绿素含量和超氧化物歧化酶(SOD)相对活性没有明显变化。而Williams和中黄35的莽草酸含量和SOD相对活性明显升高,叶绿素含量明显降低。结果表明草甘膦(浓度0.92 kg·hm~(-2))处理下,不同抗性栽培大豆叶片中莽草酸含量有明显差异,处理5~14 d时敏感材料叶片中莽草酸含量保持较高水平,因此栽培大豆叶片莽草酸含量可以作为判断其对草甘膦抗性高低的主要生理指标之一,而SOD的活性和叶绿素含量可以作为判断栽培大豆对草甘膦抗性的辅助生理指标。     

优质稻中浙优8号在惠安县种植表现及高产栽培技术

中浙优8号是中国水稻研究所和浙江勿忘农种业有限公司选育的优质高产晚籼杂交稻,2013年通过福建省引种认定。2015—2016年引进惠安县黄塘镇种植,表现生长整齐,分蘖力强,穗大粒多,产量高,米质优,适应性好,抗病性强,适合当地种植推广。总结了中浙优8号在惠安县种植表现及高产栽培技术。     

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.     

内生菌与雷公藤细胞悬浮共培养的生理生化特性

将2株雷公藤内生真菌Fusarium oxysporum NS33与Penicillium steckii NS6、2株内生细菌Enterobacter cloacae sub sp LG3与Serratia marcescens LY1及其组合分别与雷公藤细胞悬浮共培养,对不同培养体系内雷公藤细胞的生长及其生理生化特征进行研究。结果显示,在共培养前期,与对照相比,接种单一内生菌株提高了细胞的干重,其中菌株NS6的促生效果最明显;而在共培养后期,无论是单一内生菌还是混合内生菌均对细胞生长具有抑制作用。内生真菌和菌株组合处理的培养液p H值有明显的升高,而内生细菌LY1则明显降低了培养液的p H值,其具有产酸性。另外,当雷公藤细胞同混合菌株共培养时,培养基中总糖消耗量是最大的,而接种单独菌株时则对细胞可溶性蛋白含量具有一定的提高作用。接种内生菌会影响雷公藤细胞的POD、CAT及SOD活性,与对照相比,接种单独菌株会更加提升POD与CAT的活性,而细胞MDA含量则明显下降。     

薄皮甜瓜新品种‘陕甜9号’

‘陕甜9号’是用‘E13’作母本,‘C20’作父本杂交育成优质抗病薄皮甜瓜新品种。植株生长势中强,果实短梨形,平均单果质量500 g,果皮光滑,黄白色,果肉浅绿至白色,中心可溶性固形物含量14.7%,耐贮运。全生育期85～100 d,果实发育期28～32 d,抗病、抗逆性较强。适应于黑龙江、河北、甘肃、新疆等地区保护地及露地栽培。