玉米抗南方型锈病基因共分离分子标记的研究

本文以玉米(Zea mays L.)抗南方型锈病自交系P₂₅和感病自交系F₃₄₉的F₁与F₃₄₉回交,并连续回交所得BC₅代的F₃₄₉抗病近等基因系（nearly isogenic lines,NILs）为材料,以RGA（resistance gene analogs）的方法,在抗病自交系中克隆出一条321 bp的特异性DNA条带。根据序列比对的结果,设计出新的引物作为分子标记,在96株BC₇、BC₄F₅NILs及其亲本和杂种F₁群体上进行鉴定,其中94株的田间抗感结果与分子检测结果一致,选择的有效率达97.9%。此标记扩增效果清晰,可重复性强,在抗玉米南方型锈病的分子标记辅助育种研究中有应用价值。     

异源八倍体小黑麦新品种(系)的丰产稳产性研究

应用高稳系数法和常规分析方法对1998~2000年贵州省八倍体小黑麦区域试验的7个参试新品种(系)在贵州不同海拔高度、不同气候生态环境和栽培管理条件下的丰产稳产性、适应性进行比较、分析。试验结果表明,中国农科院提供的122、劲松75、劲松77、劲松76和贵州省农科院提供的黔中7号平均产量在182.8~206.24kg/667m2之间,比劲松49(ck)增产7.76%~21.58%;其中,黔中7号已于2006年通过贵州省农作物品种审定委员会审定。目前,该品种已在贵州省15个县、区以及生态相似的西南、西北等省、区试种和大面积推广应用;122、劲松75、劲松77等品种(系)已在我国西北、西南、新疆、内蒙等省、区作为饲料和粮食兼用型品种(系)应用于生产。研究表明,高稳系数法和常规分析两种方法结合应用能更准确反映八倍体小黑麦新品种(系)的丰产稳产性。     

水稻植株通气系统与稻田CH4排放相关性研究

水稻植株是稻田甲烷(CH₄)排放的重要通道,90%的CH₄气体由水稻植株通气组织排放到大气中。探讨水稻植株通气组织与水稻CH4排放量之间的相关性,可为水稻栽培和育种实践中减排CH₄提供理论和实践依据。2005年早、晚2季大田试验测定了16个水稻品种CH₄排放量和植株地上部各节间组织1     

小麦品质性状分子标记多重PCR体系的建立

小麦高分子量谷蛋白亚基组成、1B/1R易位、籽粒硬度、直链淀粉含量和穗发芽抗性等性状与加工品质密切相关，建立相关性状的多重PCR体系在小麦品质分子育种中具有重要意义。本研究利用现有品质性状基因的分子标记，建立了适合不同品质类型品种评价和分子聚合育种的3个多重PCR体系，并用已知基因的品种（系）进行验证。多重PCR体系Ⅰ包括ω-secalin（1B/1R）、Vp1B3和Pinb-D1b基因的分子标记检测，可用于一般的品质检测；体系Ⅱ包括ω-secalin、Ax2*、Bx17和Dx5 基因的检测，可望用于强筋小麦品种的选育；体系Ⅲ包括Wx-A1、Wx-B1和Wx-D1位点的检测，可用于淀粉品质或糯小麦的选育。每个体系内的引物之间不存在相互抑制作用和错配，检测品种（系）的结果可靠、重复性好，成本低。3个多重PCR体系用于小麦品质育种的亲本评价和杂交后代优质基因的聚合，将会提高优质专用小麦品种评价和选育的效率。     

New Design of Pre-amplification Circuit for EEG Signals

In the measurement of EEG signals ,the pre - amplification electrical signal of brain is the most importantant and key link. Whether the design is excellent or inferior is related to the electrical signal detection system of brain directly. The design of this circuit has been paid attention to by domestic and international expert all the time. On the basis of existing biological electricity amplifier which is studied, the authors consult the electric amplifier circuit of living beings designed , such as reference , improve and design a new-type and high performance electrical signal characteristic of brain in . The circuit has prevented the traditional design from enlarging the miscellaneous simulation of the circuit and straining the wave link, otherwise, this circuit is of simple structure , low cost, and convenient debugging. The paper has detailed theoritic analysis and experimental results to prove this circuit, which offered a new train of thought for reseach and development of brain electrical amplifier and other faint biological electricity amplifiers . This curcuit has be expected to win extensive application.     

Non-invasive Measurement of the Brain Edema and Hematoma

A new method is introduced to measure the conductivity of brain. This method uses magnetic excitation to induce eddy currents inside the brain matter and measures the magnetic field caused by the induced eddy current. The theoretical basis of this method is analyzed and the measurement system is introduced. The aufhors do the experiment and draw a conclusion that the method provided is feasible.     

Compensation of Dead Time Effects Based on the New Switching Strategy

The dead-time inserted into the switching signals of inverters case the dead-time effect of output voltage distortion. The influence of dead-time on output voltage in inverters is analyzed in detail, Then, a new switching strategy, which can provide excellent inverter output voltage distortion correction, is proposed. At last, the experiment results of inverter are presented to demonstrate the practicability and validity of the control strategy.     

Finite Groups With 52 p Elements of Maximal Order

The authors discuss the finite groups with 52 p elements of maximal order,and get a theorem as follows: SupposeG is a finite group with |M(G)|=52 p elements of maximal order,where p is a prime and p>5,then G is solvable.     

棉花品种间苗期钾吸收效率的差异研究

在水培条件下研究了转基因抗虫棉中棉所41和常规棉中棉所36、中棉所35三个品种苗期钾吸收效率的差异及其机制.结果表明,在营养液中钾浓度为0.5 mmol·L-1,可使棉花幼苗生长速率达到最高生长速率80%左右的条件下,中棉所35和中棉所41在6～7叶期的生物量和体内钾吸收量显著高于中棉所36.中棉所35的吸钾量多主要与其根系活跃吸收表面积大有关;中棉所41的吸钾量多可能是相对较大的根系活跃吸收表面积和相对较高的Imax综合作用的结果.中棉所36的Imax虽然显著高于其它两个品种,但体内钾累积量却比较低,这与其较小的根系活跃吸收表面积有关,该品种体内较高的钾浓度也可能对吸收产生反馈抑制作用.在本试验条件下,转基因抗虫棉中棉所41的苗期钾吸收能力与常规棉相比并不低.     

DPC与DTA-6复配对转基因抗虫棉苗期生长发育的调控

无论浸种还是叶面喷施,与单独使用DPC相比,DPC与DTA6复配在促进转基因抗虫棉幼苗根长和侧根原基增加的同时,显著减弱了DPC对地上部生长的延缓作用,中棉所30下胚轴长度较对照仅缩短3%左右,中棉所30、新棉99B和sGK321的苗期叶面积均相当于对照的93%～94%,这可能与复配调节剂处理后转基因抗虫棉幼苗各部位的IAA浓度较DPC单独处理明显提高有关。DPC与DTA6复配还可显著增加转基因抗虫棉苗期功能叶叶绿体的基粒类囊体垛叠程度,提高光合作用。