Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.     

柳栎高效造林关键技术研究

柳栎树形优美,且抗高温和寒冷气候,是目前国内最具前景的多用途树种。考察了立地类型、栽植方式和苗木地径规格对柳栎造林成活率及苗高、地径生长的影响,结果表明:选择地径2.0 cm裸根苗,通过宽窄行栽植能有效提高柳栎造林成活率,最大造林成活率高达91.86%。在出圃幼苗地径规格相同的情况下,柳栎造林成活率宽窄行栽植行植均匀栽植。栽植方式和出圃苗木地径规格均对造林后柳栎苗高及地径生长产生显著影响。     

重组猪抑制素蛋白质中毒素残留检测及其理化稳定性

为探索纯化的重组猪抑制素蛋白质毒素残留情况以及重组猪抑制素蛋白质稳定性,本研究利用鲎试剂、气相色谱和SDS-PAGE方法对重组猪抑制素蛋白质中的细菌内毒素、β-巯基乙醇的残留量及其蛋白质在不同温度、极端p H值和反复冻融条件下的稳定性进行检测。结果显示,经过3次等电点沉淀洗涤后,重组猪抑制素蛋白质中细菌内毒素和β-巯基乙醇的残留量大幅减少。通过在不同温度下的处理发现,重组猪抑制素蛋白质在4℃环境下较稳定,可以长期保存;但在37℃时,稳定性快速下降,随着温度上升而降解加速。通过极端p H值和反复冻融试验发现,重组猪抑制素蛋白质对极端p H值和反复冻融具有较好的耐受性。     

MOOCs的教学资源优势及对地方高校教学改革的借鉴简

“大规模网络开放课程”（MOOCs），在近一两年受到国内外教育界的关注，不同于传统教学，基于网络的MOOCs具有开放性、大规模性、互动性和透明性的特点，对我国地方高校课程资源优化与教学创新等方面具有深刻的借鉴意义。     

绵羊毛囊及其角蛋白研究进展

毛囊是毛纤维的生长基础,决定毛纤维的性状,角蛋白是毛囊组成的重要成分之一。近年来,对毛囊生长发育和角蛋白基因的挖掘等取得了一定的进展。文章回顾了绵羊角蛋白基因的发现,已经与人类角蛋白基因相当,但在数量和分类上存在差异;笔者介绍了绵羊毛囊的形态学结构,简述了毛囊角蛋白基因的表达定位和影响毛纤维性状的角蛋白,并分析了毛囊中的蛋白质组学技术的应用对毛囊生长发育和毛纤维性状的重要作用,以期为绵羊毛囊生长发育和羊毛性状的分子选育提供新的研究思路。     

湛江市园林水景的水生植物配置现状及分析

通过对湛江市几个滨水绿地的实地调查,详细研究了湛江市主要园林水景中水生植物的主要形态特性及其在园林中的应用,并讨论了其应用现状及存在的不足。     

加强综合管理 提高仔兔成活率

仔兔的适应能力和抵抗力都较差,饲养管理稍有不慎,都会造成仔兔的死亡,从而降低养兔的经济效益。综述了母兔产前准备及产后的管理,认为加强母兔的饲养管理是养好仔兔的基础以及提高仔兔成活率的保障。     

microRNA在骨骼肌发育中的功能及其表达的营养调控

microRNA( miRNA)是真核生物中一类参与调控基因表达的非编码小分子RNA,它通过翻译抑制和使靶mRNA降解,在基因表达中起着负调控作用,与骨骼肌发育、疾病等密切相关.营养可以调控miRNA及其靶基因的表达,这将为更深层次诠释营养素的作用机理提供新的研究方向.本文综述了miRNA的结构和作用机制、骨骼肌miR...     

食用菌产业助力脱贫攻坚与乡村振兴实证分析

以连云港市食用菌产业推动连云港经济发展为例,阐明食用菌产业助力乡村脱贫攻坚和农民致富的显著效果。食用菌产业的发展促进了当地农业增产增效,带动了农民增收致富,凸显了其重要的社会经济效益和生态效益。在政府扶贫支持的带动下,发展食用菌产业助力脱贫攻坚与乡村振兴已在连云港市基本实现。