巨桉纸浆原料林土壤养分与林木生长研究

通过相关分析及回归分析，探讨了巨桉人工林土壤养分与林木生长的关系，结果表明：土壤速效N、速效P、土壤有机质的浓度与林木胸径、树高、材积极显著正相关，土壤全N、全P与林木胸径树高、材积显著正相关。建立了胸径、树高、材积与土壤养分的线形回归方程，最佳数学表达式说明速效N、速效P是影响巨桉林生长的关键土壤养分。     

湖南省土地荒漠化和沙化监测布局与监测方法

我省土地荒漠化和沙化监测主要包括3个荒漠化监测站和5个沙化监测站，布设监测样地28个。确定了采集监测区域内气象、土壤、水文、生物等方面的信息和方法。     

供水工程影响下中国北方地区耕地后备资源开发潜力

灌溉水源是北方地区耕地后备资源能否有效开发的关键。当前,中国正在推动国家水网工程规划建设,为北方地区耕地后备资源开发提供了新的机遇。在以往研究基础上,该研究考虑北方地区已建、在建及规划的供水工程,基于自然适宜、利用高效、发展稳定三方面评价准则,结合三维魔方空间分类方法,开展了雨养情景和供水灌溉情景下耕地后备资源开发潜力分析。结果表明：雨养农业情景下,北方地区勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积分别为2.9万、1.0万和0.6万km²。灌溉农业情景下,耕地后备资源面积明显增加,勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积将分别达到5.4万、7.6万和6.5万km²,主要集中于新疆、内蒙古和甘肃3省（自治区）。该研究结果可为中国耕地后备资源开发利用及国家水网工程效益发挥提供参考。     

毛花雀稗混播草地建植技术初探

试验探讨了建植毛花雀稗混播草地的最佳草种组合和播种量,结果表明,毛花雀稗(30cm×30cm≈7000株)+黑麦草(每667m2为0.5kg)+白三叶(每667m2为0.15kg)在独山气候条件下的生产性能显著高于其他组合。     

硝酸钙和IAA对温州蜜柑果实钙含量及其品质的影响

在花期、花后1周、3周、采前2周对龟井蜜柑进行Ca(NO3)2、IAA和IAA+Ca(NO3)2喷布处理,同时测定了不同发育时期各处理果实钙含量及其采后果实的主要品质,并与采后浸钙处理和完熟采收果实品质进行了对比分析,结果表明:1)各处理的果皮钙含量在花后51d和112d均有所提高,但此期整个果实钙含量并无显著性差异,采收时各处理整个果实和果肉钙含量均显著高于对照;2)采收时各处理果实的可溶性固形物和可滴定酸均显著低于对照,可溶性糖、单果重、果皮色差值和相对电导率与对照无显著性差异,各处理糖酸比显著高于对照;3)采后30d时IAA+Ca(NO3)2喷布和采后浸钙处理果实的维生素C及完熟采收果实的可溶性固形物和可溶性糖均显著高于对照和其他处理。     

不同砧木嫁接网纹甜瓜试验

为预防网纹甜瓜枯萎病等土传病害,以新土佐、灰甘瓜、全能铁甲南瓜,甬砧3号甜瓜为砧木,以当地主栽的拿玻里网纹甜瓜为接穗,进行嫁接试验。结果表明:拿玻里网纹甜瓜嫁接后,植株的抗病性有了显著提高,对枯萎病的防效高达100%,对白粉病的防效也有所提高,综合抗性增强。其中甬砧3号在4个参试砧木品种中,与接穗拿玻里网纹甜瓜的亲和性最好,抗病性提高明显,品质也较自根苗好。     

Mast cell tryptase and asthma

As an important mediator of allergic inflammation, mast cell tryptase is involved in the in duction of hypersensitivity, infiltration of inflammatory cel s and t issue remodeling in respiratory tract.The effects of tryptase inhibitors on the actions of tryptase show further the potential of tryptase in the pathogenesis of asthma and its inhibitors in the treatment of asthma.     

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.