水稻籽粒镉积累KASP分子标记LCd-38的开发与利用

水稻(Oryza sativaL.)是我国主粮作物之一,稻米镉污染对我国粮食安全造成一定威胁.镉低积累水稻品种的选育可有效降低水稻籽粒镉污染风险,高效准确的镉积累相关分子标记在镉低积累水稻品种选育中具有至关重要的作用.以水稻微核心种质为材料,基于籽粒镉积累关联基因的功能性SNP位点开发了KASP分子标记LCd-38.选...     

IFN-γR和ER在大鼠下丘脑中的共存

为了探讨神经-免疫-内分泌网络中生物活性物质间相互调节和平衡的关系,本试验应用免疫组化双标记法对成年SD雌性大鼠下丘脑中IFN-γ受体（IFN-γR）和雌激素受体（ER）的共表达进行了研究。结果发现,IFN-γR和ER广泛存在于大鼠下丘脑中,其中在视前交叉上核、视前内侧核、室周核、交叉上核、下丘脑前核、下丘脑内侧核、下丘脑外侧核、乳头体前背侧核、乳头体前腹侧核等13个核团中存在大量的IFN-γR和ER双标记细胞,双标记细胞约占全部阳性标记细胞的60．9％;双标记细胞胞质呈黑褐色、胞核呈棕黄色;ER单标着色细胞以神经胶质细胞居多。研究结果表明,IFN-γ和雌激素可以分别以其各自受体为介质进行信号传递和信息整合,同时也通过在同一细胞中的相互作用而参与机体的神经-免疫-内分泌调节。     

黏红酵母发酵降解豆粕粉研究

以豆粕粉为原料。筛选优良微生物为发酵菌株进行发酵,将豆粕中大分子蛋白降解成小分子肽。通过双缩脲法测定小肽含量,并以其为指标对发酵条件进行优化。结果表明：以黏红酵母为发酵菌株,在豆粕粉6％,氯化钠0．3％,葡萄糖2％的液体发酵培养基中接种7％菌龄为18h的液体种子,于30℃、220r／min条件下,摇瓶发酵48h,发酵液中小肽含量达56．96％．比优化前提高了36．09％。     

平茬对内蒙古典型草原小叶锦鸡儿群落植物的影响

为了了解平茬对灌丛群落草本植物的影响,本文以内蒙古典型草原小叶锦鸡儿（Caragana microphylla Lam）群落为研究对象,设置了不平茬、一年平茬1次和一年平茬2次共3种处理,分析了平茬前后群落特征与植被化学计量特征的变化。结果表明：与未平茬相比,两种平茬处理下群落内禾本科植物密度均显著增加,平茬第二年禾本科生物量显著高于未平茬处理;平茬后两年内草地群落物种多样性指数随平茬次数增加均有增加趋势,且一年平茬2次处理时,Margalef丰富度指数显著高于未平茬处理;草本群落总生物量随平茬次数增加呈现先下降后增加的变化趋势;平茬后植被C、N含量、C/N比无显著变化,但植被N/P比显著增加。可见,平茬处理改变了小叶锦鸡儿群落的结构特征,提高了群落物种多样性及禾本科植物的密度,有助于典型草原的植被恢复。     

贵阳市奶牛小区奶牛繁殖性能调查

本文就贵阳市具有典型代表性的某奶牛小区奶牛的繁殖性能进行调查,调查结果表明：该奶牛养殖小区2007～2009年的平均年受胎率为83.0%,产犊间隔时间为427.00±3.36d,产后子宫复旧时间为39.94±1.72d;产后瘫痪、胎衣不下、子宫内膜炎及死胎的发病率均有逐年下降的趋势,但乳房炎和肢蹄病的发病率却呈上升的趋势。该奶牛养殖小区的胎衣不下、乳房炎、子宫内膜炎及肢蹄病的发病率仍较高,严重影响了该小区的经济效益。     

扎布耶盐山羊大肠杆菌分离鉴定及药敏试验

为掌握西藏自治区仲巴县帕江乡扎布耶盐山羊的发病情况,采集发病羊的粪便、血液、肝脏、脾脏、胸腔、腹腔和心包腔内蓄积的浆液性纤维性渗出物等进行病原菌分离鉴定及药敏试验。经病原菌分离鉴定,该地区扎布耶盐山羊羔羊感染的病原菌为大肠杆菌,检出率为97.14%(34/35);药敏试验结果表明,该研究分离得到的大肠杆菌对头孢唑林、青霉素、阿莫西林、头孢他啶、氟苯尼考、头孢拉啶6种药物高度敏感。该调查研究为仲巴县帕江乡扎布耶盐山羊大肠杆菌病的防治提供了诊断方法和用药方案。     

Study on Nutritional Characteristics of Cottonseed Hull as a Feed of Sheep

In this paper through digestive and metabolic experiments studied the voluntary intake and apparent digestibility of cottonseed hulls (kernel-contained CH,kernel-free CH) and cornstalk on sheep to evaluate the nutrition value and digestion characteristics of cottonseed hulls.12 of adult Xinjiang merino rams were selected and divided into two groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,fed with kernel-free CH by 400 (group 1) or 800 (group 2) g/(head·day) respectively,to estimate the digestibility of CH by difference method;As well,another 18 of adult Xinjiang merino rams were selected and divided into three groups (n=6 each group),and on the basis of 300 g/(head·day) of mixed concentrate,cafeteria feeding with kernel-contained CH (group 3),kernel-free CH (group 4) or ground cornstalks (group 5) respectively,to study the effects of three diets on the voluntary intake,digestion and metabolism of sheep.The results showed that the apparent digestibility of the DM,OM,CP,cellulose,hemicellulose and energy of kernel-free CH by sheep were 46.71%,47.37%,-53.40%,66.60%,62.48% and 42.59%,respectively.There were no significant differences in the intake of DM,OM,cellulose,hemicellulose,energy and Ca between kernel-contained CH and kernel-free CH groups (P> 0.05);Intake of CP and P in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);Intake of lignin in kernel-contained CH group was extremely significantly lower than that in kernel-free CH group (P< 0.01).There were no significant differences in the apparent digestibility of DM,OM and energy between kernel-contained CH and kernel-free CH groups (P> 0.05).The apparent digestibility of cellulose and hemicellulose in kernel-free CH group were significantly and extremely significantly higher than that in kernel-contained CH group (P< 0.05;P< 0.01),respectively.The apparent digestibility of CP in kernel-contained CH group was extremely significantly higher than that in kernel-free CH group (P< 0.01).The apparent digestibility of DM,OM,CP,cellulose and energy in ground cornstalks group were extremely significantly higher than that in kernel-contained CH and kernel-free CH groups (P< 0.01),however,the apparent digestibility of Ca and P were lower than that in kernel-contained CH and kernel-free CH groups (P> 0.05).Intake and retention of nitrogen in kernel-contained CH group were extremely significantly higher than that in kernel-free CH group (P< 0.01);The was no significant difference in the retention of nitrogen between kernel-free diet group and cornstalk diet group (P> 0.05).It was concluded that CH was a kind of special forage with larger intake,higher digestibility on the fiber substances and energy,but with a serious obstruction on protein digestion;The nutritional value between kernel-free diet and cornstalk diet was almost equal,and with lower nitrogen digestibility on kernel-free diet.     

对内蒙古鄂尔多斯高原全球第1148号国际重要湿地的展望

The Taolimiao-Alashan Nur(T-A Nur),of about 10km2 a brackish lake located on the Ordos upland of western Inner Mongolia,northern China,used to be not only supporting the largest breeding colony of the Relict Gull(Larus relictus)but also being a very good sample of the carrying capacity of a semi-desert wetland locality for those waterbirds,both breeders and migrating vagrants,of the region,and,therefore,the T-A Nur was recognized,in 2002,the No.1148 Ramsar Site of the world.     

草食家畜母体营养对子代生命健康影响的研究进展

母体营养对胎儿基因表达的调节作用逐渐成为围产期生命营养科学研究的热点。妊娠期作为发育可塑性的关键窗口,甲基供体对胎儿发育的相关代谢基因起到修饰作用,表观遗传学可能涉及其中的分子机制。动物研究表明,母体营养供给不足或过剩都会限制胎儿的生长。结合国内外研究报道,探讨了草食家畜妊娠期母胎的营养需求、激素在稳态调节中发挥的作用以及妊娠期能量需求对妊娠健康的重要意义,概括了妊娠期草食动物营养在表观遗传学方面的研究进展,探讨了表观遗传相关技术在草食家畜育种中的应用,以期为制定草食动物妊娠期最佳营养策略、提高胚胎质量、培养高质量后代提供参考。