宁麦9号与扬麦158株高及其构成因素的遗传解析

宁麦9号与扬麦158是我国长江中下游麦区的主栽品种和骨干亲本,长江中下游麦区近3年来审定品种中80%都是其衍生后代,研究其性状的遗传具重要意义。以宁麦9号与扬麦158为亲本构建的包含282个家系的重组自交系群体为材料,利用Illumina 90k芯片对群体进行基因型分析,建立高密度遗传图谱。连续3个生长季对株高及节间长度、穗长等株高构成因素进行测定,结合遗传图谱对株高及相关性状进行QTL定位,获得14个控制株高及其构成因素的稳定表达位点。通过进一步位置比对,聚焦到6个染色体区段,初步明确了各节间对株高的遗传调控机制。同时,将6个染色体区段中同源性较低的连锁标记转化为适用于高通量筛选的KASP标记,利用101份区域试验材料进行标记效应验证,结果显示聚合Qph-2D与Qph-5A.1两个位点具有较高的选择效率,继续聚合Q2A后,中选材料显著减少,可能降低选择效率;对Q2A与Q5A两个一因多效位点的选择建议以降低株高的等位变异为主;Qd1-5D可作为穗下节间(D1)的选择标记对株高展开优化选择。期望以上结果能为长江中下游麦区的小麦株高遗传改良提供帮助。     

Heat Transfer Model and Application of Buried Tube Heat Exchanger for Ground Source Heat Pump

This paper introduces the theoretical basis and application method for heat transfer model of buried tube (pipe in pipe and U- type) heat exchanger for ground source heat pump, and a practical example is calculated. Then, the calculation results are analyzed and discussed. In particular, the correlation coefficient and calculation method of tube group exchanger is discussed; the method for determining known parameter and some basic data for main procedure GSHP.HTM is analyzed. The calculation formula and data sampling for JSW.HTM subroutine is introduced. At last, the problems in application of calculation procedure, to which should pay attention, are discussed.     

Analysis of Essence of Urban Public Space from Viewpoint of Energy Aggregation

This paper aims at analysis on essence of urban public space from viewpoint of energy aggregation and puts forward the conception of potential energy field through the study on universal significance of energy exchange, reflection of important characteristics of energy exchange in urban public space form, static state and development about typical urban pubic space-street and square. Corresponding methods of urban design and conception of planning under the contemporary era are studied.     

Simulation for Logistics in Steel-making Process of Panzhihua Iron and Steel Corporation

To solve potential scheduling problems in production after renewal of continuous casting process in Vanadium-extracting and Steel-making Plant of Panzhihua Iron and Steel Corporation (PISCO), how to model and simulate logistics system in steel-making processhas been discussed. A series of simulatingtests for logistics system in steel-making processhave been conducted based on inputs of presentsteel-making processof PISCO. In the tests, cases of regular schedule case and cases with repairing converter have also been regarded as essential simulation condition. It has been shown that three casters can not start at same time in order to ensure continuous hot metal supply for casters when three kinds of steel are made. It has also been shown that the simulation system for logistics system in steel-making processis valid and can be used for simulating logisticsin upcoming 100% continuous casting process of PISCO. The better schedule scheme of production and reasonable match of converter-caster in time and capacity can be achieved with the help of using simulation approach.     

Examining brain oedema through brain electrical impedance topographic mapping

Change of Brain Electrical Impedance has great relations with brain oedema and brain block, a new method of Examining Brain Oedema through Brain Electrical Impedance Topographic Mapping(BEITM)is reviewed. Network topology structure that used in BEITM are also presented. According to topology, we discuss the drawing algorithm that based on triangle element. Some patients have been measured to make clinical data, according to this data Brain Electrical Impedance topographic come into being by computer at last, functional imaging has been implemented. Clinical case analysis compared with change state of an illness and repeatability of steady patient's data has proved the method is feasible.     

延缓荞麦褐变的研究进展

荞麦收获经贮藏后,感官品质和营养品质随着贮藏时间的延长发生一系列的变化。褐变是最明显的品质劣变特征,很大程度上影响加工和食用的品质及商品价值。围绕荞麦色泽变化、品质劣变、控制措施展开讨论,分析了国内外荞麦褐变过程中的研究进展,为进一步研究荞麦贮藏提供参考。     

花铃期增温对棉花干物重累积的影响及其生理机制

以遗传背景相近的棉花(Gossypium hirsutum L.)品种泗棉3号和泗杂3号为材料,于2010年在南京农业大学牌楼试验站(118º50′E, 32º02′N)模拟全球温室化气候,研究花铃期增温对棉花干物质累积的影响及其生理机制。结果显示,增温2~3℃(龄期日均温33.5~35.2℃)的条件下,主茎功能叶SPAD值降低,蒸腾速率(T_r)、气孔导度(G_s)、胞间CO₂浓度(C_i)上升,但净光合速率(P_n)下降;棉铃对位叶可溶性蛋白含量大幅上升(Δ%>50%)、可溶性糖及可溶性氨基酸含量小幅下降(P<0.05)、C/N值显著下降(P<0.05);叶片POD、CAT活性大幅下降、MDA含量显著上升;泗杂3号相关指标变化幅度较泗棉3号小。说明尽管2~3℃的增温幅度较小,但在花铃期日均温(33.5~35.2℃)的条件下,植株已处于显著的热胁迫状态,光合产物累积能力受到抑制,棉铃对位叶光合产物输出能力显著下降, 棉株总干物质累积量下降20%左右;增温条件下植株水分吸收能力下降并因此受到一定程度的水分胁迫,但此条件下光合能力下降主要由非气孔因子所致,与叶片膜结构的严重受损关系密切;泗杂3号抗高温能力高于泗棉3号。     

水稻颖花自然开放过程中茉莉酸(JA)生物合成的变化

外源茉莉酸(JA)及其甲酯(MeJA)对水稻、小麦、黑麦和高粱等禾本科植物颖花开放具有强烈的诱导效应,然而内源JA是否参与颖花开放的调控目前还缺乏充分的证据。本研究应用高效液相色谱-串联质谱(HPLC-MS/MS)系统检测了粳稻武运粳7号和籼稻明恢63颖花自然开放过程中JA水平变化,并以实时定量反转录PCR (real-time RT-PCR)技术分析了武运粳7号颖花开放过程中JA生物合成途径关键基因表达变化。结果发现,水稻颖花JA水平的变化与开颖进程相一致。JA水平在上午颖花开颖前较平稳,开颖时急剧上升至峰值,闭颖后又下降。开颖时JA水平的峰值比开颖前1 h提高4~5倍。与JA水平变化相对应,催化JA生物合成途径关键步骤同工酶的编码基因OsDAD1-3、OsLOX-RCI1、OsAOS1、OsAOC和OsOPR7的表达在开颖时会有不同程度的上调,闭颖后又下调。同一稻穗上正在开放的颖花JA含量及上述JA生物合成途径基因的表达水平也比已开放和未开放的颖花高。颖花开放时JA水平及其生物合成基因表达的上升和闭颖后的下降有力地表明内源JA参与水稻颖花开放的调控。     

拮抗酵母菌对果蔬病害防治的研究进展

在阐述应用于果蔬生物防治的拮抗酵母菌的特点与种类的基础上,综述了酵母菌对果蔬病害的拮抗机理与提高拮抗酵母菌生物防治效果方法的研究进展,分析了生物抑菌剂商业化应用的局限性与拮抗酵母菌研究与应用的发展前景。     

用A-PAGE鉴定谷子遗传多样性

蛋白质是结构基因编码的产物 ,其组成不因环境条件的改变而改变 ,可以作为对其编码的结构基因的标记。大量研究表明 ,禾谷类作物种子贮藏蛋白具有相当大的多态性 ,不同品种贮藏蛋白的电泳谱带存在较大差异 ,因此提供了一种表征基因型差异的有效而简便的方法[1～ 3] 。谷子种子的主要贮藏蛋白是醇溶蛋白 ,占种子总蛋白的4 5 %～ 6 0 % [4 ] 。在研究材料很少的情况下 ,碱性检测条件下谷子醇溶蛋白十二烷基磺酸钠 聚丙烯酰胺凝胶电泳 (SDS PAGE)检测出的遗传差异很小或几乎没有[5,6 ] 。近年来 ,禾谷类作物种子醇溶蛋白以酸性聚丙烯酰胺凝…