浑善达克沙地植物蒸腾特征的研究

采用 L i-1 60 0型气孔仪测定了浑善达克沙地主要建群乡土植物种不同季节的蒸腾速率及其环境因子的日进程 ,结果表明 :浑善达克沙地中榆树、黄柳、圆叶桦、砂杞柳、羊草蒸腾速率的日变化均呈单峰曲线 ,而叉分蓼和楔叶茶子呈双峰曲线。同一种植物在不同样地土壤水分条件下 ,蒸腾速率不同。各植物种蒸腾速率的季节变化明显 ,7、8月份蒸腾速率高 ,6月份最低。各植物种中 ,黄柳为低蒸腾植物。回归分析表明 ,影响蒸腾速率最主要的因子为光照强度 ,其次为气温和空气相对湿度 ;蒸腾速率与各因子呈线性相关 ,其关系可用方程 Tr=a+b RH+c T+d Q表示。通过蒸腾速率的比较 ,提出了浑善达克沙地植被恢复应以建立“人工疏林草原”为主。     

Expression of alpha-smooth muscle actin in scar tissue and its relationship with apoptosis

AIM: To examine the expression of alpha-smooth muscle actin in scar tissue, and observe the phenomenon of apoptosis and its involvement in the process of pathological scarring and the presence of myofibroblasts or absence of cell in the dermis. To investigate the potential role of reparative cell apoptosis in hyperplastic scar formation. METHODS: The samples of scar were obtained from post-burn patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. TUNEL assays were performed to evaluate the number of apoptotic cells in scar versus normal skin. In situ hybridization and immunohistochemistry staining technique were employed to determine the expression of different dermis cells markers in scar tissue and normal skin. RESULTS: There existed evident difference in apoptotic cells in the dermis between scars tissue and normal human skin. The expression positive cells were much more in hyperplastic scars than that in normal human skin; the apoptotic cells of proliferative stage were slight more than that of mature stage. However, in proliferative stage, the number of apoptotic cells was higher for the combination of hyperplastic scar than normally healed flat scars. But in mature stage, no obviously difference was detected between hyperplastic scar and normally healed flat scar. The monoclonal anti-α smooth muscle actin (ASMA) expression was significantly stronger in proliferative stage than that of mature stage. CONCLUSIONS: With reconstitution of dermal tissue, myofibroblasts containing alpha-SM actin disappear under normal wound healing, probably as a result of apoptosis. The myofibroblast play a critical role in wound closure and in the pathologic sequelae of healing.     

奥芬达唑和阿苯达唑对猪囊尾蚴作用形态学比较观察

观察了奥芬达唑和阿苯达唑对猪体内及体外培养的不同发育阶段囊尾蚴作用的形态学效果.结果表明奥芬达唑具有显著的疗效,优于阿苯达唑,且对未成熟期猪囊尾蚴的杀灭作用优于成熟期.提示奥芬达唑可能成为抗未成熟期猪囊尾蚴及治疗脑囊虫病的有效药物.     

芽孢杆菌BC98-Ⅰ抗真菌物质的初步分离及特性

BC98-Ⅰ是一株分离自沤肥浸渍液的蜡状芽孢杆菌，对蔬菜、瓜类、花卉枯萎病具良好的防治效果。它分泌产生的抗真菌物质的粗提物对黄瓜、西瓜、青椒枯萎菌有很强的拮抗作用，当浓度为10mg／mL时，抑菌圈直径分别达到20．33、20．22和22．77mm。该粗提物对高温高压、紫外光和蛋白酶均不敏感。同时，它在中性偏碱(pH8～9)的条件下活性最高；在极性溶剂如水、甲醇、乙醇中溶解度大，抑菌活性高。粗提液经Sephadex G100层析后，得到了两个洗脱峰，活性物质存在于第一峰中。抑菌机制试验表明：它可造成孢子萌发率降低、菌丝生长形态异常、分支加剧并产生大量泡囊。     

内蒙古农牧交错带饲草资源饲用价值评价

对地处内蒙古农牧交错带的林西县饲草资源进行了营养成份分析及饲用价值评价,结果表明:苜蓿粗蛋白质含量高于灌木类及青贮玉米,其营养动态变化规律是随生育期的变化粗蛋白质含量呈下降趋势,粗纤维含量呈上升趋势,各种成份在茎叶中的含量也随生育期变化而变化;灌木类饲用植物粗蛋白质含量15.69%～18.48%,青贮玉米粗蛋白质含量7.64%～11.15%。     

高+Gx过载作用对恒河猴肺脏的影响

15只健康恒河猴于清醒状态下，在动物离心机上经受相应峰值( 1Gx、 15Gx、 18Gx、 21Gx)的抛物线型过载作用后，按要求在过载后不同时期剖解，大体观察、取材，进行病理形态学的定性研究。结果显示：(1)眼观病变。 15Gx组、 18Gx组和 21Gx组在过载作用后即刻肺脏出现了不同程度的气肿、萎陷、淤血和出血点或斑；恢复组可见肺脏边缘有不同程度气肿区域，肺脏的背面呈暗红色；在各叶的背面均可见大小不等、多少不一的暗红色出血点或斑。(2)光镜下可见各急性实验组动物的肺脏呈现不同程度的气肿区和萎陷区，肺泡壁毛细血管扩张充血，肺泡腔内有浆液和红细胞渗出； 21Gx组还出现了血管内溶血、细支气管粘膜脱落和出血等，肺脏的病理损伤随G值升高而明显加重； 15Gx作用造成的肺脏损伤在1个月后基本恢复。而 21Gx作用后1个月肺脏的损伤尚未恢复，且出现了白细胞浸润、浆液渗出和增生等炎症反应。因此，高 Gx过载可引起猴肺脏明显的病理性损伤，其中 15Gx造成的损伤相对较轻，且容易恢复，而 21Gx造成的损伤严重，且难以恢复。     

日光温室彩椒双干循环整枝栽培技术

采用双干循环整枝技术充分发挥了彩椒潜伏芽结果的能力,在其他管理措施相同的条件下每667 m2产量达12 000 kg以上,比常规双干整枝增产30%左右。     

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.