Suppression of development of Callosobruchus maculatus (F.) (Col.: Bruchidae) in bambara groundnut seeds exposed to solar heat in the Nigerian savanna

The effect of solarization on the development of Callosobruchus maculatus was investigated in the Nigerian savanna during the hot season from April to May, 1999. Development of C. maculatus adult progeny was completely suppressed in seeds of bambara groundnut, Vigna subterranea bearing bruchid eggs or harbouring first and second instar larvae that were exposed to the sun in metal tins, clay pots or polypropylene sacks for 7, 14 or 28 h. Adults of C. maculatus developed only in seeds that were not exposed to sun. Solarization did not have a significant adverse effect on germinability of bambara groundnut seeds.     

非无菌操作下分离尖孢镰刀菌的培养基

 尖孢镰刀菌(F.oxysporum)是一类非常重要的土传病原真菌,寄主范围广,可引起100多种植物的萎蔫型病害。     

体外培育赤眼蜂研究进展

体外培育赤眼蜂的国内外研究已达到相当平：随着人工饲料配方的优化，选用的人工卵壳材料更适合于赤眼蜂的寄生与发，以及人工卵卡机的研制成功和性能不断改进，产业化体外培育赤眼蜂已成为可能。体外培育赤眼蜂的品质管理问题是实现产业化体外培育赤眼蜂的关键。然而，国内外对体外培育赤眼蜂的品质管理研究涉及甚少，有待进一步研究。本文指出应开展体外培育赤眼蜂生物学特性、行为学特性和分子生物学特性的研究，并应用于品质管理，建立体外培育赤眼蜂的品质管理体系，促进实现体外培育赤眼蜂产业化的进程，从而满足农业生产上对赤眼蜂种群的大量需要。另外，人工卵滞育贮存法的突破有助于实现体外培育赤眼蜂的产业化，也应加强研究，最后，本文对体外培育赤眼蜂的应用前景进行了展望。     

抗稻瘿蚊品种多抗1的抗性遗传分析及抗性基因定位

稻瘿蚊是亚洲稻区主要害虫,采用抗虫品种进行防治是最理想的方法。1993～1995年,广东省农科院与国际水稻研究所有关专家紧密合作,对能抗华南4个稻瘿蚊生物型的品种多抗1作进一步抗性遗传分析,确认多抗1对中国稻瘿蚊生物型1和4的抗性受显性单基因控制,这个基因暂定名为GM—6(t)。以多抗1×丰银占1组合的F3代160个家系作基因标记,据DNA库分离个体分析(BSA)原理,用随机扩增多态性DNA(RAPD)标记物OPM6(1.4kb),首次成功地标记了这个抗性基因。随后多态性扩增产物经~(32)p标记,用作探针,检测另一个参考作图群体IR64×Azucena,将这个抗性基因定位在水稻第4条染色体上,位于RG214和RG163两个DNA限制性片段长度多态性(RFLP)标记之间。应用这些分子标记辅助选择有可能不必通过稻瘿蚊的直接筛选,快速准确地选育抗稻瘿蚊品种或进行抗性基因累加。     

The effect of phenylamide fungicides on the control of potato late-blight (Phytophthora infestans) in England and Wales from 1978 to 1992

During the period 1978–1992, phenylamide fungicides in co-formulation with the dithiocarbamate fungicide mancozeb were tested for the control of potato late-blight in 51 separate field experiments in England and Wales. Whilst there was a general trend over all the experiments which indicated that foliage blight was less severe where the phenylamide + dithiocarbamate mixture had been used, the benefit was more marked in some than in others. Despite the detection of phenylamide resistance, at some sites the mixture gave better control of foliage blight than the dithiocarbamate alone. At one site where continuous data were available, the additional benefit of the phenylamide compound for control of foliage infection was lost after 1986 coinciding with a rise in phenylamide resistance from 31.7% in 1986 to 81% in 1987. Where foliar blight epidemics occurred, yield responses to fungicide programmes compared with unsprayed controls ranged from 0 to +118.5% with a mean response of +30.2%. This is equivalent to 30.8 and 12.92 t/ha respectively. Standardized yield differences were calculated to allow inter-trial and inter-year comparisons and showed no benefit from the phenylamide fungicide applied at 14-day intervals in 33 out of 38 experiments where foliar blight epidemics occurred. At one site, Cusum analysis of standardized yield differences following treatment with the phenylamide + dithiocarbamate mixture and the dithiocarbamate alone showed a mean benefit from the phenylamide mixture of 2.23 t/ha during the period 1978 to 1986. From 1987 to 1992, the yield benefit dropped to a mean level of 0.68 t/ha a decrease of 69.5%. In experiments where blight was not recorded, fungicide treatments had no deleterious effect on yields. Over all the trials, there was no effect of fungicide treatment on the incidence of tuber blight at harvest.     

Production of syringomycins and syringopeptins by Pseudomonas syringae pv. atrofaciens

All virulent strains of Pseudomonas syringae pv. atrofaciens produce in vitro substances with syringomycin-like features. All strains inhibited the growth of Geotrichum candidum in the plate assay although the extent of their growth inhibition was variable.
Purification of bioactive culture extracts of a highly virulent strain by ion exchange chromatography (Whatman CM52) yielded a main fraction which inhibited the growth of Rhodotorula pilimanae and Bacillus megaterium , and was phytotoxic to tobacco and wheat plants. In particular, the injection of this fraction in the culm of wheat plants caused phytotoxic symptoms on leaves similar to those caused in nature by the pathogen. The further purification by HPLC of the above fraction gave rise to four main bioactive substances which have been identified by spectroscopic methods (FAB-MS) and amino acid analysis as syringomycin E, syringomycin G, syringopeptin 25A and syringopeptin 25B, toxic lipodepsipeptides thus far recognized to be produced by most strains of P. syringae pv. syringae . The injection of both syringomycin E and syringopeptin 25A in wheat leaves caused necrotic symptoms; however, syringopeptin 25A was at least six times more active than syringomycin E. The possible role of the toxins in the disease development on cereals and the need for a careful examination of pathogenetic and biochemical features of P. syringae pv. atrofaciens to establish the relationships of the two pathovars in the 'syringae group' are discussed.     

The potential for selecting wheat varieties strongly competitive against weeds

The competitive abilities of a wide range of genotypes of wheat (Trilicvm aestivum L.) and durum wheat (Triticum durum Desf.) against Lolium rigidum Gaud, (annual ryegrass) were examined 1o determine the potential for breeders to select strongly competitive varieties, Considerable potential within the wheat genome to breed varieties with greater competitive ability was demonstrated. In 1993, 250 genotypes from around the world were screened and in 1994 a subset of 45 (mainly Australian) genotypes were further examined. A uniform density of L. rigidum reduced grain yield of wheat by up to about 80% in 1993 and to 50% in 1994, depending on wheat genotype. Reduction in grain yield was correlated with L. rigidum dry matter. Wheats varied in competitive ability with source, and durum wheats were less competitive than T. aestivum. The ‘old’ standard wheat varieties (released between 1880 and 1950) suppressed the weed more than all the current varieties, with the exception of eight F₁ hybrids. A doubling of the crop seeding rate of 10 of the genotypes in 1994 reduced the biomass of L. rigidum by an average of 25% compared with the standard seeding rate. Ranking of competitive ability of varieties at high density was consistent at both seeding rates. The strongly competitive genotypes had high early biomass accumulation, large numbers of tillers, and were tall with extensive leaf display. The potential for breeding enhanced competitive ability in wheat is discussed.     

Ovipositional response ofHelicoverpa armigera to different cotton genotypes

The ovipositional response ofHelicoverpa armigera (Hübner) to different cotton varieties and species was studied under caged conditions. Maximum oviposition (51.6 eggs/female) was recorded on LH 900, a variety ofGossypium hirsutum, and minimum oviposition (3.0 eggs/female) on G 27, a variety ofG. arboreum. Oviposition, in general, was low (3 to 6.5 eggs/female) onarboreum cottons as compared withhirsutum. Of the number of factors found to affect the oviposition ofH. armigera, the trichome length on the upper surface of the leaf, rather than the density, was positively correlated (r = 0.687*). Among different months in the cropping season, oviposition was maximum (552.7) during April. Oviposition was higher on leaves than on other plant parts.     

Parry's disease of pears is similar to pear decline and is associated with mycoplasma-like organisms transmitted by Cacopsylla pyricola

Mycoplasma-like organisms (MLOs) were detected by fluorescence microscopy in a number of pear cultivars and pear seedlings growing under a range of different conditions. In the cultivar Conference, MLOs were consistently associated with symptoms of Parry's disease, a decline-like disease of young trees with quince rootstocks. MLO-free pear seedlings rapidly became infected when they were planted outside. Experimental transmission of MLOs to pear seedlings and Conference trees was achieved using pear psyllids, caught outside or raised on infected plants under controlled conditions. Conference trees in an orchard trial remained free from the severe spring symptoms of Parry's disease when they were protected from feeding insects during spring of the previous year. MLOs were graft transmissible, but were not perpetuated by the standard propagation practices of budding or grafting when quince rootstocks were used. Parry's disease appears to be similar to pear decline, an MLO-induced disease well established in several other parts of the world. It is suggested that Parry's disease should be referred to as pear decline.