Embryo Production in Superovulated Goats Treated with Insulin Before or After Mating or By Continuous Propylene Glycol Supplementation

Seventeen adult and cyclic Moxoto goats were synchronized using 60 mg MPA vaginal sponge for 11 days and 50 μg cloprostenol, 48 h before sponge removal, and superovulated with 120 mg pFSH i.m. in decreasing doses at 12 h intervals for three consecutive days. In seven goats, 0.2 IU/kg BW/day of long acting insulin was subcutaneously injected at same time as pFSH, and in the other five goats, the same dose of insulin was injected for three consecutive days starting 24 h after mating. Finally, five goats were supplemented with an oral dose of 80 ml/goat/day of propylene glycol continuously during the experiment. The animals were flushed at 7 days after mating and the embryos were classified based on International Embryo Transfer Society criteria. Blood samples were collected every 3 days for insulin assay. Administration of insulin raised the insulin levels of the goats (p < 0.05), whereas in the group treated with propylene glycol, insulin rate was different only between FSH treatment and after mating (p < 0.05). Similar rates of recovery for total (80.05 ± 9.78%) or transferable structures (61.03 ± 15.13%) were obtained. Treatment was not influenced (p > 0.05) by responsiveness to superovulation, which averaged 64%. By contrast, insulin treatments were shown to increase the number of embryos considered excellent with respect to goats supplemented with propylene glycol (p < 0.05). When insulin was given before mating, a strong relationship (r = 0. 90) (p < 0.05) between number of transferable embryo and ovulations was observed in the animals. In conclusion, superovulated goats treated with low doses of exogenous insulin resulted in an enhancement in embryo quality, which was related to changes in circulating insulin concentrations.     

Estimating the potential role of commercial over‐harvesting in resource viability: a case study of five useful tree species in South Africa

There is a growing commercialization of non‐timber forest products (NTFPs) as a means of livelihood by rural communities throughout the developing world. This often occurs in the absence of any clear understanding of or guidelines regarding sustainable yields and ecological impacts, which may undermine the success of NTFP enterprises, especially from arid regions. This paper reports on the use of size class profiles and three quantitative indices to examine population profiles of five potentially useful tree species used as NTFPs in the semiarid lowveld of South Africa. We also contrast the population densities of the five tree species in 2003 with data from 1992. Low stem densities and population profiles indicated that three of the five species would preclude the establishment of NTFP enterprises based on their products. The other two species seem to have sufficient densities for some harvesting to take place, within an adaptive management framework. However, the longitudinal data indicated that the density of both these species had significantly declined over an eleven‐year period, highlighting the need for appropriate management institutions. Additionally, the proportion of mature stems cut, and the degree of cutting per stem, had increased for all five species over the eleven years. The three quantitative indices of population stability were not correlated with one another, and hence provided a useful suite of measures sensitive to different aspects of size class profiles and their interpretation. Copyright © 2005 John Wiley & Sons, Ltd.     

CASE REPORT AND CLINICAL REVIEW: Acute lameness associated with osseous metastasis of a peri-renal carcinoma in a horse

We present a case of aggressive metastatic carcinoma in a horse that was initially presented for shoulder lameness. Although radiography and scintigraphy were useful for localising a lesion in the proximal humerus, subsequent development of non-specific signs of systemic disease prompted further evaluation. Haematology and blood biochemistry, urinalysis and ultrasonography were all instrumental in identifying renal involvement. A diagnosis of a peri-renal mass causing secondary renal failure prompted euthanasia of the horse because of the poor prognosis. Antemortem findings were supported by necropsy, with secondary lesions also identified in the spleen, liver, 8th left rib and proximal humerus. Histological examination yielded a diagnosis of undifferentiated metastatic carcinoma.     

Pairing and phase separation in a polarized Fermi gas

We report the observation of pairing in a gas of atomic fermions with unequal numbers of two components. Beyond a critical polarization, the gas separates into a phase that is consistent with a superfluid paired core surrounded by a shell of normal unpaired fermions. The critical polarization diminishes with decreasing attractive interaction. For near-zero polarization, we measured the parameter beta = -0.54 +/- 0.05, describing the universal energy of a strongly interacting paired Fermi gas, and found good agreement with recent theory. These results are relevant to predictions of exotic new phases of quark matter and of strongly magnetized superconductors.     

Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry

The use of frozen semen in the swine industry is limited by problems with viability and fertility compared with liquid semen. Part of the reduction in sperm motility and fertility associated with cryopreservation may be due to oxidative damage from excessive or inappropriate formation of reactive oxygen species (ROS). Chemiluminescence measurements of ROS are not possible in live cells and are problematic because of poor specificity. An alternative approach, flow cytometry, was developed to identify viable boar sperm containing ROS utilizing the dyes hydroethidine and 2', 7'-dichlorodihydrofluorescein diacetate as oxidizable substrates and impermeant DNA dyes to exclude dead sperm. The percentage of sperm with high mitochondrial transmembrane potential was determined by flow cytometry using the mitochondrial probe 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethylbenzimidazolylcarbocyanine iodide with propidium iodide staining to exclude nonviable cells. Sperm were incubated with and without ROS generators and free radical scavengers. Basal ROS formation was low (less than 4%) and did not differ (P = 0.26) between viable fresh and frozen-thawed boar sperm. In addition, fresh and frozen-thawed viable sperm were equally susceptible (P = 0.20) to intracellular formation of ROS produced by xanthine/xanthine oxidase (94.4 and 87.9% of sperm, respectively). Menadione increased (P < 0.05) ROS formation, decreased (P < 0.05) JC-1-aggregate fluorescence intensity, and decreased (P < 0.05) motion variables by 25 to 60%. The mechanism of inhibition of motility by ROS formation may be related to a decrease in mitochondrial charge potential below a critical threshold. Catalase and superoxide dismutase treatment in the presence of xanthine/xanthine oxidase indicated that hydrogen peroxide was the primary intracellular ROS measured. Further, catalase, but not superoxide dismutase, was capable of attenuating ROS-induced inhibition of motility. Whereas basal intracellular hydrogen peroxide formation was low in viable fresh and frozen-thawed boar sperm, both were quite susceptible to external sources of hydrogen peroxide.     

Recovery of aquatic bacterial populations in a stream after cessation of chemical pollution

Tims Branch was studied for 1 yr to determine the effects of an annual loading of about 229 900 kg of various chemicals on the bacterial populations of a small fast flowing stream. The 10 yr discharge of chemical waste to this stream was terminated in 1973 and a 2 yr study of the stream's recovery was begun. Upper Three Runs, an adjacent nonpolluted stream, was used as a control. Total culturable bacterial counts, per cent chromagenic bacteria and diversity were used as indices of recovery. Plate count agar was used for culturing aquatic bacteria present in both systems. Diversity in both systems was more affected by seasonal temperatures than the presence of chemical pollution. Mean total counts dropped from 187 × 10³ in the polluted stream to approximate those of the control stream (170 × 10³) following cessation of pollution. In the chemically affected stream, per cent chromagens increased from 15% during pollution to 25% approximately 1 yr after pollution was stopped. The latter percentage was comparable to that of the control stream (28%) during the period of study.     

Metastatic malignant melanoma in a prehensile-tailed porcupine (Coendou prehensilis)

A 14-yr-old male, prehensile-tailed porcupine (Coendou prehensilis) presented for an ulcerated, bleeding lesion of the right flank. The wound presented similar to a bite wound and was treated with antibiotics. After 2 mo, the lesion had increased in size and was nonhealing, so surgical excision was elected. Histopathology diagnosed this lesion as a malignant melanoma with incomplete margins. Radiographs showed no evidence of pulmonary metastasis. At 6 mo, another skin lesion was removed and was diagnosed as malignant melanoma with clean surgical margins. At 8 mo, another four dermal masses were surgically excised and, again, these were melanomas that were completely excised. The animal was euthanized approximately 15 mo after initial presentation due to continued growth of dermal masses, dyspnea, and decreased appetite. Necropsy and histopathology revealed metastatic melanoma present in skin, kidneys, and lung.     

Size Dependence of Structural Metastability in Semiconductor Nanocrystals

The kinetics of a first-order, solid-solid phase transition were investigated in the prototypical nanocrystal system CdSe as a function of crystallite size. In contrast to extended solids, nanocrystals convert from one structure to another by single nucleation events, and the transformations obey simple unimolecular kinetics. Barrier heights were observed to increase with increasing nanocrystal size, although they also depend on the nature of the nanocrystal surface. These results are analogous to magnetic phase transitions in nanocrystals and suggest general rules that may be of use in the discovery of new metastable phases.