动物梨形虫线粒体研究进展

线粒体在真核生物以及单细胞生物的生命活动中发挥着重要作用。寄生虫为适应不同的生存条件进化了一套自己的方式,包括线粒体基因组成、基因表达和能量代谢方式。梨形虫（巴贝斯虫和泰勒虫）是一类重要的胞内寄生虫,给畜牧业造成重大的经济损失。本文对梨形虫为适应宿主而进化出的一些特征进行了描述,为寻找防治梨形虫的新途径提供理论依据。     

Construction of Larval cDNA Expression Library and Immunological Identification of Positive Clones in Tick Haemaphysalis qinghaiensis

A primary cDNA expression library with a titer of 5.0 × 105 PFU mL-1 was constructed from mRNA extracted from larval Haemaphysalis qinghaiensis ticks in order to identify certain genes,which would then be used as candidate molecules for development of effective vaccines to control this parasite.Totally 11 positive clones,which designated as HqL01-11,were obtained by immunoscreening of the library using a polyclonal antibody generated in rabbit with larval tick protein extract.Results of sequence analysis from BLASTN searching revealed that 6 of them had no significant homology with the adult H.qinghaiensis ticks’ known genes,4 of them had no significant homology with all genes deposited in GenBank database.HqL07,HqL08,HqL09,and HqL11 were deposited to GenBank database,and accession numbers were EF605263,EF605264,EF605265,and EF605266,respectively.Subsequently,HqL07 and HqL09 were expressed in vitro and the molecular weights of the corresponding expressed products were 60 and 70 kDa,respectively.Western blot analyses showed that HqL07 and HqL09 had immunogenicity.This study laid the foundation for future production of genetically engineered vaccines for the immunological control of H.qinghaiensis.     

粳稻台中65及其F1花粉不育近等基因系和它们的F1花药培养特性

利用粳稻台中65及其F1花粉不育近等基因系和它们的F1作研究材料,对这些遗传背景基本相同但具有不同F1花粉不育基因座位材料进行花粉愈伤组织诱导率及花药开裂性的研究．结果为：亲本与杂种在离体条件下的花粉愈伤组织诱导率无显著差异,杂种F1花粉部分败育并未导致花粉愈伤组织诱导率下降,表明F1花粉不育基因与花粉愈伤组织诱导能力性状无直接联系;但亲本与杂种间的花药裂药性却存在显著差异,并随着花粉败育率的提高花药开裂率下降,表明F1花粉不育基因与花药开裂性状有关．