苹果金纹细蛾的生物学特性及药剂试验

苹果金纹细蛾（Ｌｉｔｈｏｃｏｌｌｅｔｉｓ ｒｉｎｇｏｎｉｅｌｌａ Ｍａｔｓ．）在鲁中南地区１年发生５～６代,以蛹在苹果落叶内越冬,翌年３月下旬是开始再现越冬代成虫,４月上旬达盛期。成虫羽化后于次日清晨５：００～６：００开始交配,持续交配１．５～３ｈ,交配后不久在叶片背面产卵,单个散产,平均单雌产卵４０余粒。幼虫孵化后直接景入叶片内取食叶肉,并非苞内化蛹。在１５～３０℃,卵、幼虫、蛹的发育历期分别为     

利用性诱剂防治花生田暗黑鳃金龟的研究

为明确暗黑鳃金龟(Holotrichia parallela Motschulsky)性诱剂对成虫的引诱效果及对幼虫虫口密度的降低作用,在全国主要花生产区开展了田间诱捕试验,调查诱捕成虫数、幼虫减退率及对花生的保果效果。结果表明:一台诱捕器一天内诱到的最大成虫数为977头;幼虫减退率最高可达78.8%,保果效果最高可达68.6%。表明暗黑鳃金龟性诱剂对成虫有很强的引诱效果,并能明显减轻幼虫为害。     

苹果短枝型品种矮化早果机制研究──营养特性

比较了在同一立地条件下生长年龄、砧木完全一致的苹果短枝型品种和普通型品种的营养特性。结果表明：（１）短枝型品种地上部的Ｎ素营养、碳素营养明显高于普通型品种，地下部根系的营养含量相反；（２）短枝型品种耐肥性较高；（３）两类品种对铵态氮和硝态氮肥的田间反应存在差异。     

牛副结核ELISA诊断方法研究

本文建立了牛副结核病ELISA诊断方法。采用亲和层析抗原。被检血清以高压粉碎草分枝杆菌吸收原进行吸收。该方法的敏感性76％,特异性97％,通过对2483头奶牛进行检测,检出率为6.1％,并与常规的补反和变态反应进行了比较。作者认为:牛副结核ELISA可以替代补反,做为检测牛副结核的一种有力手段。     

抗牛布鲁氏菌独特型抗体杂交瘤细胞（Ｆ９）的生长和代谢

用RPMI1640培养基对抗牛布鲁氏菌独特型抗体杂交瘤细胞株(F9)进行培养，并对其生长代谢进行测定。该细胞不论接种1.1×10     

浅谈二噁英的检验技术

1　检测二口恶英的技术难度食品和饲料中二口恶英及其类似物的检验属于超痕量级检验,通常以ｎｇ/ｋｇ和ｐｇ/ｋｇ表示。它比其他污染物含量低千倍到亿倍以上,检测难度很大,其多组分即同系物、异构体的化学前处理技术几乎包罗近代最先进的手段,涉及分离、富集、提取、净化、清洗和浓缩装置,还包含自动凝胶色谱(ＡｕｔｏｍａｔｅｄＧｅｌＰｅｒｍｅａｔｉｏｎＣｈｒｏｍａｔｏｇｒａｐｈｙ)、反相ＨＰＬＣ、玻璃色谱柱等。检测过程需要尖端大型分析仪器的支持,定量分析依靠ＨＲＧＣ/ＨＲＭＳ,对仪器的灵敏度、选择性和特异性要求极高,是现今分…     

苏丹红和饲料着色剂的区别

本文主要从苏丹红和饲料着色剂的化学结构、作用机理及毒性等方面说明饲料着色剂与苏丹红的天壤之别。     

Impacts of a freedom farrowing pen design on sow behaviours and performance

The limited space in farrowing crate imposes many challenges, such as prolonged farrowing duration and high piglet stillbirth rate. Although the features of farrowing pens compensate for the drawbacks of farrowing crates, they are associated with high piglet crushing mortality caused by the greater space afforded to sows and their rolling-over behaviour. Therefore, a freedom farrowing pen was designed to overcome the drawbacks of both farrowing crates and farrowing pens. The main features of the freedom farrowing pen are its left anti-crushing bar and detachable right anti-crushing bar on the sides of the sow lying area. It also has a 10 cm-high anti-crushing bar in the non-lying area. Eighteen healthy, multiparous Yorkshire sows (3-7 parity) were averaged and randomly assigned to farrowing crates, farrowing pens, and freedom farrowing pens to compare the effects of the farrowing systems on sow behaviour and performance. Results showed that the farrowing duration and the mean piglet birth intervals were longer for the sows in farrowing crates than for those in farrowing pens and freedom farrowing pens (P<0.05), but there was no difference between the sows in farrowing pens and those in freedom farrowing pens (P>0.05). The piglet stillbirth rate was higher for the sows in farrowing crates than for those in farrowing pens and freedom farrowing pens (P<0.001). Crushing mortality was higher among piglets in farrowing pens (P<0.001), but there was no difference between piglets in freedom farrowing pens and those in farrowing crates (P>0.05). The freedom farrowing pen and the farrowing pen allowed sows to turn around and move freely, but because of the different structures of their anti-crushing bars, the increase in sow movement did not cause higher piglet crushing mortality (P>0.05). Sows in freedom farrowing pens were found to be more protective of their piglets.     

Effects of road transportation on metabolic and immunological responses in Holstein heifers

This study examined the effects of road transportation on metabolic and immunological responses in dairy heifers. Twenty Holstein heifers in early pregnancy were divided into non‐transported (NT; n = 7) and transported (T; n = 13) groups. Blood was collected before transportation (BT), immediately after transportation for 100 km (T1) and 200 km (T2), and 24 h after transportation (AT). The T heifers had higher (P < 0.05) blood cortisol and non‐esterified fatty acid concentrations after T1 and T2 than did NT heifers. By contrast, the T heifers had lower (P < 0.05) serum triglyceride concentrations after T1 and T2 than had the NT heifers. The serum cortisol and triglyceride concentrations returned (P > 0.05) to the BT concentrations at 24 h AT in the T heifers. The granulocyte‐to‐lymphocyte ratio and the percentage of monocytes were higher (P < 0.05) after T2 in the T heifers than in the NT heifers, suggesting that transportation stress increased the numbers of innate immune cells. T heifers had higher (P < 0.01) plasma haptoglobin concentrations than NT heifers 24 h AT. In conclusion, transportation increased cortisol secretion and was correlated with increased metabolic responses and up‐regulation of peripheral innate immune cells in dairy heifers.     

锰的添加方式对肉仔鸡生产性能的影响

试验选用1日龄AA肉仔鸡180只,随机分为3组。Ⅰ组为对照组,添加硫酸锰（MnSO4·H2O）120mg／kg;Ⅱ组添加蛋氨酸螫合锰60mg／kg;Ⅲ组分别添加蛋氨酸螯合锰（Met-Mn）30mg／kg和硫酸锰（MnSO4·H2O）60mg／kg。研究不同方式添加锰对肉仔鸡生产性能的影响。结果表明．3种不同方式添加锰对肉仔鸡生产性能无显著影响（P〉0．05）．但Ⅲ组的饲料成本有所降低。因此,在肉仔鸡日粮中以第3种方式添加效果最佳。