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821.
Karin Themann Sabine Werres R. Lüttmann H.-A. Diener 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(4):337-343
Samples of water and sediment were taken from drains, reservoirs and wells from four commercial hardy ornamental nurseries with water recirculation systems. The samples were taken on seven different dates throughout a single year from August 1994 to July 1995. The samples were screened for Phytophthora species using five different methods: direct plating, three bait tests (using lupin seedlings, apples and Rhododendron leaves) and a DAS-ELISA (double-antibody sandwich enzyme-linked immunosorbent-assay) with two antisera. In the nurseries with old water recirculation systems, Phytophthora species were detected in the drains and in the reservoirs. In the nursery with a new recirculation system, the pathogens were only present in the drains. None of the water samples from wells in any of the nurseries were contaminated. Phytophthora species were present in the water as well as in the sediment samples from drains and reservoirs. They were detected in the water recirculation systems irrespective of the season. The number of isolates increased about sevenfold between late summer and spring. At least 12 different Phytophthora species were identified: some isolates were previously unrecorded species. The epidemiology of the pathogens in outdoor water recirculation systems as well as the importance of the results for commercial nurseries is discussed. 相似文献
822.
Cynodon dactylon is a low-growing C4 weed that is highly sensitive to shading. This species shows severe biomass reductions and highly plastic morphological changes in response to resource availability. The objective of this study was to analyse the effect of shading on the spatial and biomass growth of C. dactylon . Two experiments were carried out exploring different environmental conditions. Six treatments were applied on C. dactylon patches. Daylight and 18%, 41%, 48%, 69% and 85% shading of daylight photosynthetic active radiation (PAR) were imposed in Expt 1 and 0%, 22%, 44%, 50%, 74% and 87% shading of daylight PAR in Expt 2. Patches were slightly elliptical in shape and grew 3.2 and 1.5 cm d−1 with 0% shading, on average, during the whole studied periods in Expts 1 and 2 respectively. Biomass was significantly reduced from 41% shading onwards in Expt 1 and from 50% shading in Expt 2. However, patch extension rate was only significantly reduced from 85% and 74% shading in Expts 1 and 2 respectively. Biomass partitioning was modified by shading in both experiments. A functional analysis of C. dactylon patches showed that the patch extension rate diminished linearly when patch biomass growth rate was lower than 1.66 g d−1 ; above this value, the extension rate remained constant. 相似文献
823.
Isoxaflutole is a new pre‐emergence herbicide for use in maize and sugarcane. Its two main derivatives are a diketonitrile derivative, the 2‐cyano‐3‐cyclopropyl‐1‐(2‐methanesulfonyl‐4‐trifluoromethylphenyl)propan‐1,3‐dione, named DKN, and a benzoic acid derivative, the 2‐methanesulfonyl‐4‐trifluoromethylbenzoic acid, named BA. The adsorption/desorption processes have never been studied for isoxaflutole (IFT) at high concentrations nor for BA, and the present work aimed at completing the knowledge of the behaviour of these three molecules in conditions close to those encountered in the context of agricultural use. The adsorption/desorption study was conducted on seven soils of different physical and chemical properties, using the batch equilibrium technique. During the experiments, IFT was chemically converted into DKN in a continuous manner. This reaction appeared to be dependent on the pH of the soil and was taken into account in the calculations of the adsorbed and desorbed amounts. The adsorption isotherms obtained were predominantly C‐shaped for IFT and DKN and S‐shaped for BA, but some differences appeared on a few soils. They fitted well the Freundlich equation, and the values of the Freundlich coefficient Kfa showed that, whatever the soil, IFT was more adsorbed than its two derivatives. The main parameter influencing the adsorption of IFT appeared to be the organic matter content, whereas this effect was not evident for DKN and BA. No correlation was found between the extent of adsorption and either clay content or pH of the soil, for the three molecules. 相似文献
824.
825.
826.
Thirty-four isolates ofFusarium oxysporum f.sp.melonis (F.o.m.) obtained from 205 fields in melon-producing areas in the southeastern Anatolia Region of Turkey were identified on the basis
of colony morphology and pathogenicity by the root dip method. In this region the mean prevalence of wilt disease was 88.1%
and the mean incidence of disease was 47.5%. Physiologic races 0, 1, 2, and 1,2 of the pathogen were determined by their reactions
on differential melon cultivars ‘Charentais T,’ ‘Isoblon’, ‘Isovac’ and ‘Margot’ in the greenhouse. Race 1,2, representating
58.8% (20/34) of all isolates, was widely distributed. Of the other pathogenic isolates, eight were identified as race 0,
five as race 1, and one as race 2. This is the first report of physiologic races ofF.o.m. in Turkey. Of 44 melon cultivars tested in the greenhouse for resistance toF.o.m. races, 36 were found to be moderately resistant to race 0, 17 were susceptible to race 1,2, 34.1% were highly resistant to
race 1, and 52.2% had moderate resistance to race 2.
http://www.phytoparasitica.org posting July 16, 2002. 相似文献
827.
828.
Summary Polymerase chain reaction (PCR) amplification of specific alleles (PASA) was adapted as a molecular marker‐based method for the rapid detection of point mutations in Amaranthus retroflexus and Amaranthus rudis leading to ALS inhibitor resistance. Two pairs of primers were designed for the specific amplification of alleles of the ALS gene of susceptible and resistant biotypes. The allele‐specific primer matched the desired allele, but mismatched the different allele at its 3′ end. Differentiation was carried out by comparison of the amplified DNA fragments in gel electrophoresis after PASA‐PCR. In A. rudis, differentiation was possible with one PCR and genomic DNA as probe. A ‘nested’ PCR was necessary for the differentiation of sensitive and resistant A. retroflexus. PASA is useful for the identification of resistant weed biotypes and also as a monitoring tool to map resistance occurrence and distribution. Advantages include the fast and clear separation of those plants with and without mutations at an early stage of development, its easy and consistent performance and quick results compared with existing resistance detection tests. These advantages, when combined with management strategies, enable further activities to reduce herbicide resistance. 相似文献
829.
Summary Orobanche species are commonly identified using morphological characteristics. In many cases, the distinction of closely related species is difficult, and a molecular tool is more suitable to differentiate them. In this study, genomic polymorphism between morphologically distinct species was investigated through amplification by polymerase chain reaction (PCR) of intersimple sequence repeat (ISSR) regions. Five primers were used to study genetic variation in the morphologically distinct species O. hederae and O. amethystea, as well as the closely related species O. cernua and O. cumana. For the first two species, all the primers detected genetic polymorphism. Anchored primers allowed the identification of more specific molecular markers than non‐anchored tri‐ and tetranucleotide primers. Genetic polymorphism was investigated among three O. hederae populations using the two types of primer. One non‐anchored and two anchored primers detected intraspecific variation, which was not correlated with the geographical location of those populations. The primer (GATA)4 detected polymorphism between five specimens each of O. cernua and O. cumana species collected from different countries, permitting these two closely related species to be clearly differentiated. This study demonstrated that ISSR markers can be highly reliable for precise identification of Orobanche species. 相似文献
830.
Ludwig John A. Eager Robert W. Bastin Gary N. Chewings Vanessa H. Liedloff Adam C. 《Landscape Ecology》2002,17(2):157-171
The cover, number, size, shape, spatial arrangement and orientation of vegetation patches are attributes that have been used
to indicate how well landscapes function to retain, not ‘leak’, vital system resources such as rainwater and soil. We derived
and tested a directional leakiness index (DLI) for this resource retention function. We used simulated landscape maps where
resource flows over map surfaces were directional and where landscape patch attributes were known. Although DLI was most strongly
related to patch cover, it also logically related to patch number, size, shape, arrangement and orientation. If the direction
of resource flow is multi-directional, a variant of DLI, the multi-directional leakiness index (MDLI) can be used. The utility
of DLI and MDLI was demonstrated by applying these indices to three Australian savanna landscapes differing in their remotely
sensed vegetation patch attributes. These leakiness indices clearly positioned these three landscapes along a function-dysfunction
continuum, where dysfunctional landscapes are leaky (poorly retain resources).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献