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91.
Two-year toxicity and carcinogenicity study of methyleugenol in F344/N rats and B6C3F(1) mice 总被引:2,自引:0,他引:2
Johnson JD Ryan MJ Toft JD II Graves SW Hejtmancik MR Cunningham ML Herbert R Abdo KM 《Journal of agricultural and food chemistry》2000,48(8):3620-3632
Methyleugenol (MEG) was tested for toxicity/carcinogenicity in a 2-yr carcinogenesis bioassay because of its widespread use in a variety of foods, beverages, and cosmetics as well as its structural resemblance to the known carcinogen safrole. F344/N rats and B6C3F(1) mice (50 animals/sex/dose group) were given MEG suspended in 0.5% methylcellulose by gavage at doses of 37, 75, or 150 mg/kg/day for 2 yr. Control groups (60 rats/sex and 50 mice/sex) received only the vehicle. A stop-exposure group of 60 rats/sex received 300 mg/kg/day by gavage for 53 weeks followed by the vehicle only for the remaining 52 weeks of the study. A special study group (10 animals/sex/species/dose group) were used for toxicokinetic studies. All male rats given 150 and 300 mg/kg/day died before the end of the study; survival of female rats given 150 mg/kg/day and all treated female mice was decreased. Mean body weights of treated male and female rats and mice were decreased when compared to control. Area under the curve results indicated that greater than dose proportional increases in plasma MEG occurred for male 150 and 300 mg/kg/day group rats (6 and 12 month) and male 150 mg/kg/day mice (12 month). Target organs included the liver, glandular stomach, forestomach (female rats) and kidney, mammary gland, and subcutaneous tissue (male rats). Liver neoplasms occurred in all dose groups of rats and mice and included hepatoadenoma, hepatocarcinoma, hepatocholangioma (rats only), hepatocholangiocarcinoma, and hepatoblastoma (mice only). Nonneoplastic liver lesions included eosinophilic and mixed cell foci (rats only), hypertrophy, oval cell hyperplasia, cystic degeneration (rats only), and bile duct hyperplasia. Mice also exhibited necrosis, hematopoietic cell proliferation, and hemosiderin pigmentation. Glandular stomach lesions in rats and mice included benign and malignant neuroendocrine tumors, neuroendocrine cell hyperplasia, and atrophy and in mice included glandular ectasia/chronic active inflammation. In female rats, the forestomach showed a positive trend in the incidences of squamous cell papilloma or carcinoma (combined). Male rats also exhibited kidney (renal tubule hyperplasia, nephropathy, and adenomacarcinoma), mammary gland (fibroadenoma), and subcutaneous tissue (fibroma, fibrosarcoma) lesions. Male rats also exhibited malignant mesotheliomas and splenic fibrosis. These data demonstrate that MEG is a multisite, multispecies carcinogen. 相似文献
92.
Judith Bender Jon R. Washington Bianca Graves Peter Phillips Godfried Abotsi 《Water, air, and soil pollution》1994,75(3-4):195-204
Microbial mats have been developed to sequester heavy metals from contaminated water. Mixed populations of photosynthetic and heterotrophic bacteria, dominated by Scillatoria spp., were developed for metal tolerance and integrated into a durable, self-sustaining community of microbes stimulated by and attached to ensiled grass. The mat was immobilized on glass wool and layered in flow-through baffled tanks. After allowing 8 weeks for the maturation of the mat, mixed solutions of Zn and Mn (15–16 mg L?1) were passed through a three-tank experimental series. Effluent from each tank was first sampled and then applied to the next tank. This procedure was repeated in triplicate and with six applications of new metal solution per three-tank series. By the third tank, the target metal concentration <1 mg L?1 was always achieved. Mean percentages of the initial influent concentration removed by tanks 1, 2 and 3, respectively, were 72, 93 and 98 for Zn and 78, 97 and 99 for Mn. Mean metal concentrations in the effluents (average of 6 applications) were, for tank 1: Zn (mg L?1) 5.0, Mn (mg L?1) 4.2; for tank 2: Zn 1.6, Mn 0.75; for tank 3: Zn 0.53, Mn 0.19. Mean effluent concentrations from each of the three sequential treatments (average of 6 applications per tank) were for Zn (mg L?1) 5.0, 1.6 and 0.53; for Mn (mg L?1) were 4.2, 0.75 and 0.19. Thus target concentrations were reached in experimental tank 2 for Mn and tank 3 for Zn. Metal removal in the control tank series, containing glass wool only, was 37% for Zn and 5% for Mn (average of 6 applications). Oxygen and redox potential analyses of the mat/glass wool matrix revealed a heterogenous structure of anoxic and oxic zones. Zeta potential analysis of the mat samples identified a mat surface charge ranging from ?12.3 to ?69.2 mV. Various metal removal mechanisms possibly involved with metal sequestering include surface binding to the mat or to mat exudates trapped within the glass wool, precipitation of the metals with anions present in the oxic/anoxic zones, mat mediation of the water conditions in favor of metal-oxide precipitation and active transport of the metals into the cell. 相似文献
93.
Introduced species may impact native species and communities in many ways. One which has received relatively little attention is by serving as resources for natives, thereby altering their ecology. We address such impacts on the California butterfly fauna as currently understood. Eighty-two of California's approximately 236 butterfly species (34%) are reported as ovipositing or feeding on introduced plant taxa. Many more utilize introduced plants as nectar sources. Interactions with introduced plant taxa are not distributed evenly among butterfly species. Alpine and desert butterflies interact with relatively few introduced plants because few exotic plant species have reached and successfully colonized these habitats. Other California butterfly species are specialists on particular plant families or genera with no exotic representatives in California and have thus far failed to recognize any introduced plants as potential foodplants. Some California butterflies have expanded their geographic ranges and/or extended their flight seasons by feeding on exotic plants. However, negative impacts of exotic plant species can also occur. At least three of the state's butterfly species currently lay eggs on introduced taxa that are toxic to larvae. Impacts of introduced plant taxa on California's butterflies are expected to increase as both habitat conversion and alien introductions accelerate. 相似文献
94.
95.
Sex in reptiles is determined by genes on sex chromosomes or by incubation temperature. Previously these two modes were thought to be distinct, yet we show that high incubation temperatures reverse genotypic males (ZZ) to phenotypic females in a lizard with ZZ and ZW sex chromosomes. Thus, the W chromosome is not necessary for female differentiation. Sex determination is probably via a dosage-sensitive male-determining gene on the Z chromosome that is inactivated by extreme temperatures. Our data invite a novel hypothesis for the evolution of temperature-dependent sex determination (TSD) and suggest that sex chromosomes may exist in many TSD reptiles. 相似文献
96.
97.
Collette BB Carpenter KE Polidoro BA Juan-Jordá MJ Boustany A Die DJ Elfes C Fox W Graves J Harrison LR McManus R Minte-Vera CV Nelson R Restrepo V Schratwieser J Sun CL Amorim A Brick Peres M Canales C Cardenas G Chang SK Chiang WC de Oliveira Leite N Harwell H Lessa R Fredou FL Oxenford HA Serra R Shao KT Sumaila R Wang SP Watson R Yáñez E 《Science (New York, N.Y.)》2011,333(6040):291-292
98.
Vitousek PM Ladefoged TN Kirch PV Hartshorn AS Graves MW Hotchkiss SC Tuljapurkar S Chadwick OA 《Science (New York, N.Y.)》2004,304(5677):1665-1669
Before European contact, Hawai'i supported large human populations in complex societies that were based on multiple pathways of intensive agriculture. We show that soils within a long-abandoned 60-square-kilometer dryland agricultural complex are substantially richer in bases and phosphorus than are those just outside it, and that this enrichment predated the establishment of intensive agriculture. Climate and soil fertility combined to constrain large dryland agricultural systems and the societies they supported to well-defined portions of just the younger islands within the Hawaiian archipelago; societies on the older islands were based on irrigated wetland agriculture. Similar processes may have influenced the dynamics of agricultural intensification across the tropics. 相似文献
99.
Tendencies of day-old chicks to respond toward, to approach, and to stay near a distant audiovisulal imprinting apparatus were shown to be inherited in a heterotic manner. High correlations found among the traits suggest that these behavioral responses are not independent but sequential. The fitness value of such a behavioral sequence is explained in terms of imprinting. 相似文献
100.
Carbon dioxide fixation by mouse embryos prior to implantation 总被引:1,自引:0,他引:1
Mouse embryos in the stage of development prior to implantation were cultured in vitro in a medium that contained radioactive bicarbonate. The radioactivity was incorporated into the proteins and nucleic acids that were acid soluble. Uptake of radioactivity occurred into protein in the unfertilized ovum and was highest in all fractions in the early blastocyst stage. No incorporation was detected in the lipid fraction. 相似文献