Impacts of diet on hindgut microbiota and short‐chain fatty acids in grass carp (Ctenopharyngodon idellus)

Diet is known to influence intestinal microbiota in fish, but the specifics of these impacts are still poorly understood. Different protein/fibre ratio diets may result in differing structures and activities of gut microbiota. We examined the hindgut microbiome of grass carp (Ctenopharyngodon idellus) fed three different diets: fish meal (FM, high protein – low fibre), Sudan grass (SG, high fibre – low protein) and compound feed (CF, intermediate). Microbial profiles of fish fed on FM were significantly different from profiles of fish fed CF and SG (F = 18.85, p < .01). Cetobacterium, known to be positively associated with protein digestion, was the dominant microbial group in FM samples (approximately 75.7%), while Lachnospiraceae and Erysipelotrichaceae, thought to be involved in fermentation of plant polysaccharides, were dominant in CF and SG samples (46.8% and 42.9% respectively). Network analyses indicated that the abundance of Lachnospiraceae and Erysipelotrichaceae was in a significantly positive correlation (r = .895, p = .001). Short‐chain fatty acid (SCFA) levels may indicate that the digestibility of diet by microbiota in the grass carp gut decreased from FM to SG (FM>CF>SG). Overall low SCFA levels indicate that hindgut fermentation probably provides a low proportion of energy requirements in grass carp.     

美洲鲥致病性维氏气单胞菌的分离鉴定及药敏试验

从自然感染发病的美洲鲥肝脏、肾脏和肠道中分离出1株细菌,经革兰染色镜检及PCR鉴定,确定为维氏气单胞菌(Aeromonas veronii)。用该菌株接种健康美洲鲥,鉴定其致病力,从人工感染50 g左右的健康美洲鲥发现,菌株具有很强的致病力,试验鱼在感染后72 h内全部死亡,且患病症状与自然发病的鱼症状一致。进一步采用药敏纸片法测定分离菌株对多种抗生素的敏感性,结果发现:维氏气单胞菌对恩诺沙星、氧氟沙星、头孢噻吩三种药物极为敏感,对红霉素、头孢唑啉中度敏感,对青霉素、氨苄西林、阿莫西林、四环素、链霉素不敏感。     

Distribution and migration of Japanese common squid, Todarodes pacificus, in the southwestern part of the East (Japan) Sea

Data on squid catches of Korean and Japan, water temperatures at depth of 100 m, and night-visible images of fishing boats collected in the East (Japan) Sea from 1970 to 1999 were analyzed to examine the distribution and migration of the Japanese common squid, Todarodes pacificus, in the southwestern part of the East (Japan) Sea. The main fishing grounds detected from squid catch in each grid (0.5° latitude × 0.5° longitude) by Korean squid fishery and night-visible images provided by the Defense Meteorological Satellite Program (DMSP) Operational Linescan System (OLS) were situated in the southwestern part of the East (Japan) Sea. The distribution and migration route was illustrated from squid catch in each grid and DMSP OLS image. In years of high catches, the fishing grounds were situated mainly between Ulleung Island and the eastern coastal waters of Korea, while in years of low catches they were situated between Ulleung Island and the Yamato Bank in the central East (Japan) Sea. The center of fishing activity began moving northward from around the Korea/Tsushima strait to the northern boundary of the Tsushima Warm Current in March, reaching into the Yamato Bank in September, and then returning to the strait by February. The northward and southward migration routes differed; the northward migration route occurred closer to the mainland coast of Korea than the southward migration route did. This work suggests T. pacificus begin their northward migration almost 2 months earlier than previously suggested.     

电液伺服遥操纵机器人主-从位置控制器

针对电液伺服遥操纵机器人主-从双向伺服位置控制中存在的非线性和不确定性,提出主-从侧分别使用带干扰观测器的最优控制和有鲁棒补偿的PD反馈控制算法,以克服主动侧各液压缸因动态特性差异造成的位置跟随误差和从动侧外界环境的随机干扰对系统稳定性的影响.分别设计了观测器跟踪扰动的二次型控制器和有动态鲁棒补偿的位置控制器,通过仿真和试验验证了所设计控制器的有效性.试验结果表明,所设计的位置控制器,既提高了主-从位置跟随精度,又具有较强的自适应性和鲁棒性;提高了力反馈电液伺服遥操纵机器人系统的操作性.     

胡萝卜微粉物理特性和营养成分的影响因

胡萝卜微粉制备中,影响微粉物理特性及营养成分的试验分析表明:热风和微波干燥获得的胡萝卜微粉容积密度大,冷冻干燥的胡萝卜微粉容积密度小.微波干燥获得的胡萝卜微粉溶水性好,其次是冷冻干燥;胡萝卜生粉比熟粉溶水性好.胡萝卜生粉的氨基酸质量比高于熟粉和冻融粉;真空冷冻干燥生胡萝卜,粉碎粒径小于240目时,氨基酸质量比高.经过冻融处理的胡萝卜微粉V_c质量比高于其生粉和熟粉;冷冻干燥的胡萝卜微粉V_c质量比高.类胡萝卜素质量比随着粒径减小而减小;冷冻干燥冻融处理的胡萝卜,在粉碎粒径为80～120目时,类胡萝卜素和V_c质量比高.     

肉脯干燥加工新技术

根据肉脯加工工艺需求,对传统热风干燥设备进行了相应的改进,并结合现代热泵(冷风)干燥设热风干燥的特点,分别进行了热泵(冷风),热风以及热泵(冷风)、热风组合干燥肉脯加工工艺的研究,以探索出一种能满足现代工业化生产需求的肉脯干燥加工新技术.     

火山岩和沸石填料BAF深度处理印染废水的效能比较

针对纺织印染行业水质不达标回用率低的问题,采用火山岩和沸石为填料的曝气生物滤池对纺织印染废水二级生化出水进行了深度处理,进行了两反应器出水效能的比较。研究表明:火山岩填料深度处理纺织印染废水的效能要高于沸石填料,建议气水比在10∶1左右,出水CODcr为36~48mg/L,出水色度35~55度,NH3-N浓度0.5~1.7mg/L,出水浊度范围0.9~3NTU。     

基于屈服强度的X80钢管100％SMYS试压的可行性

高强度试压是国内外管道试压的普遍趋势.为研究我国X80钢管100％SMYS(最小屈服强度)试压的可行性,以西气东输二线西段为例,应用概率统计方法,分析了X80直缝钢管和螺旋缝钢管管材试样屈服强度的分布特征,并研究了实物屈服强度和管材试样屈服强度的差异.结果表明:X80直缝钢管和螺旋缝钢管试样的屈服强度分布均符合正态分布,X80直缝钢管屈服强度性能优于螺旋缝钢管,钢管实物屈服强度比管材小试样屈服强度高10％～15％.以100％SMYS试压导致母材屈服概率小于1％为目标,根据管材试样屈服强度概率分布特征及试样与实物屈服强度的差异,得出X80钢管可进行100％SMYS试压的结论.     

浙江近海曼氏无针乌贼卵巢周年变化的研究

曼氏无针乌贼Sepiella maindroni de Rochebrune在中国东、黄渤海均有分布。近年来，其产量不断下降。因此开展乌贼生物学的研究，合理地利用、保护乌贼资源已引起人们的注重。关于乌贼繁殖生物学，仅对其繁殖习性作了些研究。曼氏无针乌贼的人工孵化近年来作过初步探讨。在乌贼性腺发育方面，对金乌贼Sepia eseulenta Hoyle作过研究。然而曼氏无针乌贼的性腺发育至今还未见报道。本文拟对曼氏无针乌贼卵巢周年变化作些观察，为乌贼的增养殖提供些基础生物学资料。