稠油降解菌的筛选及其对胶质和沥青质生物降解

以稠油为唯一碳源,从辽河油田石油污染土壤、渣油中,经富集培养,分离筛选出细菌菌株11株、真菌23株和放线菌10株。将在含油培养基上长势良好的菌株进行室内摇瓶实验,发现真菌的降解效果好于细菌,F2006的降解效果最好,14d去除率达到39.8%。在胶质和沥青质污染土壤中,观察真菌F4、F2008、F9902、F2006、F2017、F6和F9904在14d、28d和42d对胶质和沥青质的去除率,测试结果表明,菌株F2006和F2008降解效果较好,在42d时去除率分别达到55.73%和57.62%。两次实验说明,菌株F2006为降解胶质和沥青质的优势菌株,在生物修复中具有较好的应用前景。     

红叶石楠嫩枝扦插育苗技术研究

鲁宾斯是红叶石楠中抗性强、相对较耐寒的品种,本文利用全光照自动喷雾装置对鲁宾斯嫩枝扦插育苗技术进行了研究。结果表明：外源激素IBA＋NAA混配成适宜的浓度,辅以合适的水分管理措施,可有效提高红叶石楠嫩枝扦插的生根率。本研究为今后红叶石楠的嫩枝扦插繁殖提供了参考依据。     

蜂斗叶组培快繁体系的建立

对蜂斗叶的组培条件进行了较系统的研究.结果表明,以蜂斗叶的叶柄切段为外植体,在MS+6-BA 2mg/L培养基上可诱导出胚性愈伤组织并分化出丛生芽;芽丛在MS+6-BA1 mg/L的继代培养基上,月繁殖系数可达3倍以上;健壮芽在1/2 MS+NAA 0.1 mg/L+活性炭1 mg/L的生根培养基上生根率为100%.     

修水县一季晚稻免耕抛秧技术要点

简要介绍了江西省修水县一季晚稻的免耕抛秧技术要点。     

冬凤兰非共生萌发和低温离体保存（英文）

[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource.     

Effects of Supplemental Histamine on Gastric Acid Secretion, Digestive Enzyme Activities, Intestinal Microfloral of Early Weaned Piglets

Two experiments were conducted to study the effect of supplemental histamine in the diet ofearly-weaned piglets. In experiment A, 24 cross bred piglets with an average body weight of 6.10±0.40 kg,weaned at the age of 28 days, were divided into four groups, fed with basal diet of low dietary copper without(control) or with supplemental histamine at 60, 120, 180 μg kg-1 BW. During the two weeks and the thirdweek after weaning, ADG(average daily gain) of piglets were increased by 15.8% (P＜0.05), 9.5% (P＜0.10) by addition of 60 μg kg-1BW histamine, but decreased by addition of 180 μg kg-1BW histamine, whichalso increased the amount of E. coli in colon and the scour incidence. The secretion of gastric acid and pepsinwere improved by both dose of supplemental histamine (60, 180 pg kg-1BW) and gastric digesta pH were de-creased by both. Addition of 60 μg kg-1 BW histamine improved the activities of trypsin, amylase in duodelumdigesta. In experiment B, 12 cross bred piglets with an average body weight of 6.85±0.35 kg, weaned at theage of 28 days, were divided into two groups, fed with basal diet of high dietary copper without (control) orwith supplemental 60 μg kg-1 BW histamine. During the two weeks and the third week after weaning, ADG ofpiglets were increased by 9.8% (P＜0.05), 7.0% (P＜0.10). The secretion of gastric acid, activities oftrypsin and amylase in duodelum digesta, were also improved by addition of 60 μg kg-1 BW histamine. The re-sults showed that addition of histamine (60 μg kg-1 BW) in early weaned piglets could increase the secretion ofgastric acid and pepsin, reduce gastric digesta pH and scour incidence, improve activities of trypsin, amylasein duodelum digesta, and the growing performance of early weaned piglets.     

双城土壤养分空间变异与玉米分区施肥技术研究

采用全球卫星定位系统(GPS)和地理信息系统(GIS)技术,对黑龙江省双城市粮田耕层土壤进行了空间变异特征和分区施肥研究.结果表明,黑龙江省西南部玉米主产区黑钙土养分含量比较丰富,硼含量较高,氮含量较低.土壤中B、N、S、P的空间分布具有明显的变异性.土壤中B元素空间分布的偏斜程度较大.土壤养分偏斜程度与土壤养分变异系数之间存在密切的线性相关关系,相关系数R=0.9674.玉米分区平衡施肥取得了显著效果,较习惯施肥平均增产851 kg/hm2,平均增产率10.9%,平均增收610元/hm2.     

牛传染性鼻气管炎病毒LN01/08株灭活疫苗的研制

为研制牛传染性鼻气管炎病毒(IBRV)灭活疫苗,本研究以国内分离鉴定的IBRV LN01/08株为种毒,优化病毒增殖条件,获得病毒滴度达108.0TCID50/mL,将其灭活制备疫苗.为比较不同免疫佐剂的效果,分别以矿物质白油和Montanide ISA206佐剂配制灭活疫苗,进行牛体免疫试验.临床观察和血清中和抗体检测结果表明,Montanide ISA206佐剂乳化的疫苗在降低副反应和增强免疫效果方面优于矿物质白油佐剂.应用Montanide ISA206佐剂制备3批灭活疫苗,并对其安全性和免疫保护效果进行测定.结果表明该疫苗安全可靠,对强毒攻击可产生较好的抵抗力,攻毒保护率达80%.疫苗在2℃～8℃保存12个月后仍能保持良好的免疫效果.     

氮素水平对油菜根茎叶氮素输出 及角果中氮素积累的影响

通过测定不同施N条件下油菜各器官的N素含量,研究了油菜后期根、茎枝、叶片等营养器官中N素的 输出和角果中N素的积累。其主要结果如下: (1)根、茎枝、叶片中N素积累量的变化呈先增后减的趋势,一般在 开花期前后达最大值;角果中N素总量随角果的生长逐渐增加。增施N肥明显增加各器官的N素积累量,推迟最 大值出现的时间。(2)根系、茎枝、叶片中的N素向外输出的比例随施N量的增加而下降,分别占其最大值的20% ～35%、35%～65%、55%～70%。(3)角果中的N素有30%左右来自叶片中N素的再利用,受施肥量的变化影响 较小; 35% ～10%来自茎枝的N素的再利用,随施N量的增加而减少;来自根系中N素的再利用不足5%;来自开 花后从土壤中吸收的N素约为30% ～60% ,随施N量的增加而增加。     

雨养垄系机械化优质高效配套技术的小麦产量及效益模型

对小麦雨养垄系机械化优质高效配套技术进行二次正交旋转组合试验 ,建立了优化产量及效益模型 ,分析了各试验因素的关系 ,筛选出优化产量及优化效益的小麦栽掊方式 ,为总结雨养垄系机械化优质高效配套技术体系的规律性提供可靠的科学依据