1. A study was undertaken to examine the effect of supplementation of diets with fats of different chain length and degree of unsaturation on the performance, carcase characteristics and tissue fatty acid composition of broilers.
2. Three hundred and sixty 19‐day‐old female broilers were fed on diets containing supplemental fat/oil (50 g/kg) in the form of beef tallow (BT) (diet T‐T), soyabean oil (SBO) (diet S‐S), rapeseed oil (RSO) (diet R‐R), marine oil (MO) (diet M‐M) or binary mixtures (0.5:0.5 w/w) of these lipids (diets T‐S, T‐R, T‐M, S‐R, S‐M, and R‐M) to 54 d of age. Food intake, live weight, plucked weight and the lipid concentration and fatty acid composition of abdominal fat pad, liver and breast muscle were measured.
3. Food intake, plucked weight and live weight were greatest for diet T‐T and lowest for diet R‐R. Food conversion ratio was poorest for birds fed on diets containing BT. Lipid concentration in abdominal fat pad was significantly higher in birds fed on diets containing SBO. liver lipid concentration was significantly reduced by diets containing RSO.
4. Abdominal fat pad fatty acid profile was most readily altered by dietary fatty acids. There was a strong correlation between dietary fatty acid composition and tissue fatty acid composition for all fatty acids except C14:0, C20:0, C20: 2n‐6 and C20: 4n‐6. The tissue P/S ratio ranged from 0.40 for diet T‐T to 1.40 for diet S‐S. The n‐6/n‐3 ratio was significantly increased by the inclusion of SBO and decreased by the inclusion of MO.
5. Liver fatty acid profile was least modified by dietary fatty acids. There was an inverse relationship between liver and dietary C20:4n‐6 concentration. Tissue C18:2n‐6 and C20:4n‐6 were highly correlated, suggesting significant interconversion by ?‐6 desaturase in this tissue. The n‐6/n‐3 ratio was significantly increased by inclusion of SBO and significantly decreased by the inclusion of MO.
6. In breast muscle MO‐based diets increased the proportion of n‐3 PUFA at the expense of n‐6 PUFA. The tissue concentrations of C18:ln‐9, C18:2n‐6, C20:ln‐9, C20:5n‐3, C22:5n‐3 and C22:6n‐3 were strongly correlated with dietary fatty acid composition. Muscle and diet P/S and n‐6/n‐3 ratios were highly correlated. 相似文献
Paleoclimate records indicate that the strength of the Asian summer monsoon is sensitive to orbital forcing at the obliquity and precession periods (41,000 and 23,000 years, respectively) and the extent of Northern Hemisphere glaciation. Over the past 2.6 million years, the timing (phase) of strong monsoons has changed by approximately 83 degrees in the precession and approximately 124 degrees in the obliquity bands relative to the phase of maximum global ice volume (inferred from the marine oxygen isotope record). These results suggest that one or both of these systems is nonstationary relative to orbital forcing. 相似文献
The first objective of this study was to evaluate intrauterine nitric oxide (NO) and endometrial inducible NO synthase (iNOS) in mares susceptible or resistant to persistent breeding‐induced endometritis (PBIE) within 24 h after breeding. Mares susceptible (n = 6) or resistant (n = 6) to PBIE were inseminated over five cycles, and uterine secretions and endometrial biopsies were collected before and 2, 6, 12 and 24 h after insemination. Uterine secretions were analysed for NO and biopsies were analyzed for iNOS expression. A second experiment evaluated the effect of treatment with dexamethasone or mycobacterial cell wall extract (MCWE) on uterine NO production and endometrial iNOS mRNA expression. Six susceptible mares were inseminated over three cycles with (i) killed spermatozoa without treatment (control), (ii) killed spermatozoa with 50 mg of dexamethasone IV or (iii) MCWE IV 24 h prior to insemination with killed spermatozoa. Six resistant mares were inseminated with killed spermatozoa as a control. Six hours after breeding, uterine biopsies and secretions were collected and evaluated for NO and iNOS mRNA. In Experiment 1, resistant mares had an increase in iNOS mRNA expression 2 h post‐breeding compared to baseline (p = 0.045), 12 h (p = 0.014) and 24 h (p = 0.001). Susceptible mares had higher expression 2 h compared to 6 h (p = 0.046). No differences were observed in mRNA or protein expression of iNOS between resistant and susceptible mares. Resistant mares had a relatively steady amount of total intrauterine NO over 24 h, while susceptible mares had an increase over time, with a significantly higher increase in total NO than resistant mares at 6 (p = 0.04) and 12 h (p = 0.032). In Experiment 2, no differences were observed for iNOS mRNA expression. Susceptible mares had increased NO when compared to resistant mares (p = 0.008) and MCWE decreased NO (p = 0.047). 相似文献
Ranched bison are typically less acclimated to handling than are domesticated livestock, suggesting that they might be more vulnerable to handling and transportation stressors. Grain-finished bison were slaughtered on-farm (n = 11), or held for 48 h, transported to a research abattoir, held in lairage for 18 h, and then slaughtered (n = 11). An additional group (n = 10) was sampled at a conventional fixed location abattoir. Measures included serum cortisol and corticosterone concentrations during on-farm handling and exsanguination, serum glucose, creatinine phosphokinase (CPK), aspartate aminotransferase (AST), and trim losses. Transport was associated with an increase in trim loss. On-farm, glucose was elevated, CPK was positively associated with handling order over 12 h, and corticosterone concentration, although lower than cortisol concentration, showed a greater response to prolonged disturbance. With appropriate on-farm handling facilities, the use of on-farm slaughter and mobile abattoir could avoid muscle damage and trim losses, and mitigate injuries sustained during handling and transport of bison. 相似文献
Five chickpea cultivars, Chitoui, Neyer, Kasseb, Beja 1 and Bouchra, were planted on three sowing dates at two Experimental
Stations in Tunisia: Bou Salem in the north and the more southerly Mornag, where the climate is drier. Severity of blight,
caused by Ascochyta rabiei, was measured on a 1–9 scale (defined) on vegetative parts and on pods as percent infected and percent infected that were
empty. At both locations, disease was essentially absent on plants sown on the third dates but present on plants sown on the
two earlier dates. At Bou Salem, disease severity was highest for the second sowing date whereas at Mornag it was highest
for the first sowing date; but for each sowing date, disease severity was lower at Mornag than at Bou Salem. Yield components
were measured as number of pods per plant, number of seeds per plant, number of seeds per 100 pods, 100 seed weight and weight
of seeds per plant. Both disease severity and yield differed significantly among sowing dates (differently at each location)
and also among cultivars for each sowing date, these differences depending both on sowing date and location. A lower yield
was always associated with a higher disease severity, although the quantitative relationship differed between cultivars and
locations. Cultivar Beja 1 had the lowest vegetative disease scores at both locations and both sowing dates 1 and 2. Beja 1
also scored well for all yield components. Plants sown on the third (latest) date gave the highest yields for all cultivars
at both locations (except for an unusually high yield of Neyer at Mornag on sowing date 2), in some instances being more than
double those from the earlier sowing dates. Thus, in contrast to other studies, late sowing did not result in yield loss. 相似文献
The objective of this study was to assess the effect of environmental variability on the dynamics of the Atlantic mackerel (Scomber scombrus L.) stock in the Gulf of St. Lawrence (GSL). We first described the dominant modes of physical and biological (zooplankton) variability using Principal Components Analyses of 40 variables. Two principal modes of variability were identified, a long‐term mode (15–20 yr) associated with a warming of the GSL and a second mode describing alternating cold and warm periods at a higher frequency (5–10 yr). A strong link between physical forcing and the dynamics of zooplankton species known to be important for mackerel was shown. Second, a set of Generalized Additive Models (GAM) was developed to explore how these environmental variations could influence mackerel condition (Fulton's K) and recruitment success (Rs). Optimal GAMs including variations in abundance, species composition and phenology of key copepods improved model performance by 40–50% relative to those considering only physical environmental conditions. The results are consistent with the match–mismatch hypothesis and illustrate the key role of zooplankton dynamics in modulating variations in mackerel K and Rs. Finally, this study showed that large variations in Rs could be caused by varying environmental conditions independently of the influence of stock biomass. Our results strongly indicate that the effect of environmental variability should be considered in the implementation of an ecosystem‐based approach to Atlantic mackerel stock management. 相似文献