Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.     

海贝柱自动晾晒设备设计

针对当前传统人工晾晒海贝柱效率低下、容易造成污染等问题。设计了海贝柱自动晾晒机械,采用阶梯式晾晒生产线对湿海贝柱分级晾晒,多条晾晒生产线分时、高效的工作,机器上方设有遮板以保证晾晒作业不被污染而且不受天气条件的影响。整机采用stm32单片机、传感器等技术实现自动化作业,机器控制系统监测到生产线发生故障或海贝柱出现堆积时会立即停止工作以便于检查故障、减少损失。试验结果表明:机械晾晒不会造成海贝柱内的营养成分的流失,机械晾晒海贝柱的干基含水率达到30%仅需12h,机械晾晒海贝柱能节省大量的干燥时间,显著的提高了晾晒效率,避免了人工晾晒过程导致的二次污染和损失。该研究为海贝柱自动晾晒机械的研制提供了技术参考。     

欧盟共同农业政策面临的挑战及最新改革措施

共同农业政策作为联系欧盟成员国农业的纽带,在经历2003年的改革后,虽然在价格补贴、农村发展等方面取得一定的成效,但仍然面临财政负担、食品安全、新成员国加入等来自内外部的挑战和压力.为应对这些挑战和压力,欧盟进行了降低政策价格、微调配额等一系列的最新改革.     

不同栽培密度对秋大棚黄瓜生育期及产量的影响

试验以黄瓜为材料,采用随机区组设计,研究了不同栽培密度对秋大棚黄瓜生育期及产量的影响。结果表明,当栽培密度为4000株/667m2时,产量和产值都达到最高。     

棉花不同时期去叶源对库源关系影响的初步研究

通过对棉花不同时期去主茎叶、叶枝叶、果枝叶和全部叶片的研究,初步探讨了棉花叶源对库的影响.结果表明:无论蕾期还是盛铃期,叶片是棉花光合产物的主要来源,没有叶片棉花就不能正常生长,也很难保证其产量;不同部位的叶片中,主茎叶的作用显著,蕾期去主茎叶后株高日增长量减少0.5 cm以上,干物质积累明显下降,盛铃期则导致棉铃大量脱落,成铃平均减少2个以上;去果枝叶的作用相对较小,去叶枝叶几乎无影响.     

合成二氢月桂烯醇的研究进展

综述了从蒎炮合成二氢月挂烯醇的路线及关键反应的研究进展，包括蒎烯氢化、蒎 烷异构化。一步法和二步法从二氢月桂烯制二氢月桂烯醇。     

一种电流模式多功能滤波器结构

提出了一种简单的电流模式多功能滤波器结构，具有一个输入端和三个输出端，可以实现低通，高通，带通，全通和陷波功能，不需要任何严格匹配关系，该结构中使用了６个ＣＣ器件，两个接地电阻和两个接地电容，结构十分简单且无源灵敏度很低。     

库存本阅览室与外借库的合并管理

我馆在2005年将中文图书库存本阅览室与中文图书外借库合并成中文书库,进行统一管理。本文结合工作实际,分析了书库合并前分开管理的弊端,阐述了二者合并的意义,提出了合并后的具体管理措施。     

Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.     

GIS在生态土地分类中的作用

This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites, a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect, vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems. The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province. Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain Forest Ecosystem. Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China Responsible editor: Zhu Hong