库存本阅览室与外借库的合并管理

我馆在2005年将中文图书库存本阅览室与中文图书外借库合并成中文书库,进行统一管理。本文结合工作实际,分析了书库合并前分开管理的弊端,阐述了二者合并的意义,提出了合并后的具体管理措施。     

Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.     

应用热脉冲技术对小美旱杨耗水量的研究

应用热脉冲对河套灌区农田防护小美旱杨（Populus popular’s）日蒸腾耗水量进行了研究。结果表明：在正常生长状态下，小美旱杨的日蒸腾耗水量变化曲线呈宽峰状，随着林龄增加，日蒸腾耗水量逐渐增加，在不同水分条件下,灌水时日蒸腾耗水量：萌生苗日蒸腾耗水量大于扦插日蒸腾耗水量。     

踏郎沙地造林技术

文章介绍了内蒙古赤峰市沙地造林树种踏郎的造林技术     

中国矮马运铁蛋白遗传多态性的初步研究

采用聚丙烯酰胺凝胶电泳对中国矮马运铁蛋白多态性进行了测定。在运铁蛋白位点共发现5种表型，即F2F2，DF2，F2H2，F2O和F2R，由Tf^D,Tf^F2,Tf^F2,Tf^H2,Tf^O和Tf^R等5个等位基因控制，其基因频率分别为0.0312,0.7188,0.0625,0.0312和0.1563；基因的杂合度（H），个体鉴别概率（Dp)和亲仔关系排除概率（PE）分别为0.4530,0.6875和0.2586。     

猪生殖与呼吸综合征病毒M基因和N基因的表达

将猪生殖与呼吸综合征病毒（PRRSV）的M基因和N基因从重组质粒pMD18-T—M—N中亚克隆至pBV220原核表达栽体上，成功构建了重组表达质粒pBVM—N。将pBVM—N转化大肠埃希氏菌DH5α感受态细胞．重组菌经温度敏感诱导表达，其细菌裂解物经SDS—PAGE可检测到分子质量约为19ku的目的蛋白，与M基因表达产物一致；经蛋白质分析软件Bandscan分析，其表达量可达19．1％；Western-blotting分析表明，该重组蛋白可被兔抗PRRSV血清所识别，与预期的M基因表达产物相一致，而N基因未获表达。     

引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种

 1995年在内蒙古临河市新发现了糖甜菜细菌性叶斑病,从病斑所分离的10个细菌菌株经柯赫氏法则验证,均确系该病的病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析、DNA G+C mol%和DNA-DNA同源性测定进行了鉴定,并与植物病原棒形细菌15个标准菌株进行了比较。该病原菌为革兰氏阳性细菌,不规则短杆状,有一根鞭毛、亚极生或侧生,结合其生理生化特性、细胞化学成分和DNA G+C mol%和DNA-DNA同源性测定结果,认为应属于短小杆菌属(Curtobacterium)的萎蔫短小杆菌(Cur. flaccumfaciens),数值分析也支持这一结论。此外,据血清学反应结果及其对短小杆菌属的其它植物寄主的致病情况,认为该病原菌应是萎蔫短小杆菌种下的一个新的致病变种,定名为Curtobacterium flaccumfaciens pv. beticola pv. nov. Chen et al.,2000(萎蔫短小杆菌糖甜菜致病变种)。     

以甜菜粕取代小麦的全混合颗粒饲料对羔羊瘤胃pH值的影响

本试验选用24只3-3.5月龄的波德代(♂)×蒙古羊(♀)和陶赛特(♂6)×蒙古羊(♀)杂交一代羔羊,按2×2×2因子设计,研究了两个营养水平下以高果胶的甜菜粕取代小麦的全混合颗粒饲料对两种羔羊瘤胃pH值的影响。结果表明,低营养水平组的pH值显著高于高营养水平组(P=0.015);食后4 h(P-0.019)、6 h(P=0.009)的pH值在营养水平和饲料间存在互作,高营养水平组,小麦组羔羊的瘤胃液pH高于甜菜粕组,而低营养水平组、甜菜粕组的pH高于小麦组;羊品种对pH值无影响。     

采收成熟度对牛肝菌保鲜贮藏的影响

不同成熟度的食用菌子实体采后生理、病理及贮藏品质有不同的变化规律 ,本文通过对美柄牛肝菌(BoletusspeciosuFrost)三种不同成熟度的子实体的呼吸强度、呼吸商的测定分析 ,及贮藏过程中菌体外观品质变化综合评定的产生及发展进行分析 ,为牛肝菌采集及贮藏中的原料标准的制定提供依据。     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.