Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.     

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.     

煤矸石栽培基质在农业中资源化利用研究现状

随着煤矸石在农业方面研究的不断深入,其作为栽培基质的开发和应用已成为重要的研究领域.该研究在梳理和分析国内外研究成果的基础上,综述了煤矸石理化及生物学特性、利用途径、已取得的主要成果及存在的问题,指出今后煤矸石在农业资源化利用中的发展趋势.     

火灾环境下邻近罐壁热辐射分布实验

油罐火灾蔓延的主要因素是油罐热辐射,在着火罐火焰热辐射作用下,邻近罐极易被引燃,造成整个罐区大面积起火。为了研究邻近罐罐壁热辐射分布规律,建立了油罐燃烧对邻近罐热辐射影响的小尺寸实验装置,开展了火灾环境下邻近罐壁热辐射分布的实验研究。结果表明:在火灾环境下,邻近罐正对着火罐方向的罐壁受辐射作用最大,热辐射从罐顶到罐底逐渐降低、从中心向两边呈轴对称降低;随着L/D(L为相邻两个油罐的距离,D为油罐的直径)增大,邻近罐热辐射下降。实验数据为火灾环境下邻近罐的热辐射研究提供了参考,对防止邻近罐被引燃,做好油罐区火灾爆炸事故的预防具有重大意义。     

冬闲地种草养鹅模式下不同鹅品种生长性能、屠宰性能、肉品质的比较

为比较冬闲地种草养鹅模式下不同鹅品种的生长发育、屠宰性能和肉品质,以28日龄四川白鹅、浙东白鹅、扬州鹅和霍尔多巴吉鹅为研究对象,采取全程草地放牧加补饲方式,每组3个重复,每个重复10只,于70日龄开展屠宰测定和肉品质测定。结果显示:①在整个生长发育期间,四个品种鹅在生长发育前期增重较大,后期趋于缓慢。在生长发育前期(28~49日龄),扬州鹅的周增重均不同程度地高于四川白鹅和浙东白鹅,但在36~42日龄时扬州鹅的体增重低于霍尔多巴吉鹅;在生长发育后期(50~70日龄),扬州鹅的体增重均高于其他三个品种鹅。②扬州鹅的屠宰率高于其他三个品种的鹅;扬州鹅的腹脂率为1.39%,显著低于其他三个品种鹅。③扬州鹅不同部位肌肉中水分含量最低,胶原含量最高;扬州鹅的胸肌中蛋白质含量显著高于其他三个品种鹅。综上所述,扬州鹅可作为种草养鹅的理想品种,研究结果为种草养鹅模式下适宜品种的选择提供了理论依据。     

冠县棉花花铃期田间管理技术

阐述了冠县棉花花铃期的生育特点,结合生产实际制定了田间管理关键技术措施,包括重施花铃肥、补施盖顶肥、中耕培土、适时整枝、化学调控、病虫害防治等,以供参考。     

‘马来西亚1号’菠萝蜜叶片氮磷钾动态的研究

通过对海南省文昌市南阳镇、琼海市大路镇、乐东县黄流镇3个地区‘马来西亚1号’菠萝蜜叶片氮、磷、钾3种营养元素的周年变化规律跟踪调查分析,结果表明：3个地区菠萝蜜叶片营养元素变化规律相似。元素年平均含量表现为氮>钾>磷。根据实际生产中的生育期调研分析,将菠萝蜜的生长周期大致划分为2个批次3个时期：第一批次养树期（11—12月）、花芽分化期（1—2月）、结果期（3—4月）;第二批次养树期（5—6月）、花芽分化期（7—8月）、结果期（9—10月）。生长周期内,氮元素含量年变化呈现“N”字型变化趋势;磷含量在1—4月呈先上升后下降的趋势,随后呈缓慢上升趋势;钾含量变化趋势与氮含量变化相似,但钾元素含量变化没有氮元素变化明显。菠萝蜜叶片中大量元素含量的适宜范围为：全氮21.03~22.15 g/kg,全磷2.86~3.40 g/kg,全钾13.49~14.47 g/kg。在生长发育过程中,菠萝蜜需氮、钾元素较多,特别是在花芽分化期和结果期,应注意增施氮肥与钾肥,磷肥应于养树期施足。