库存本阅览室与外借库的合并管理

我馆在2005年将中文图书库存本阅览室与中文图书外借库合并成中文书库,进行统一管理。本文结合工作实际,分析了书库合并前分开管理的弊端,阐述了二者合并的意义,提出了合并后的具体管理措施。     

Influence of all-trans retinoic acid on proliferation and differentiation of neural stem cells from new born rat striatum

AIM: To study the effect and mechanism of all-trans retinoic acid (atRA) on neural stem cell (NSCs) proliferation and differentiation from new born Sprague-Dawley rat striatum. METHODS: NSCs were isolated from the brains of new born Sprague-Dawley rat striatum, and the features of cells were characterized by immunofluorescence staining. The effects of different culture medium on cell cycle distribution and proliferation of NSCs were determined by flow cytometry (FCM). The effects of atRA on differentiation of NSCs were determined by immunofluorescence staining and classified count of differentiated cells. RESULTS: FCM assay indicated that atRA inhibited the proliferation of NSCs. The percentage of cells in G0/G1 phase in atRA treatment group was significantly higher than that in control, and the proliferation index (PI) was significantly low. The percentage of neurons differentiated from NSCs in atRA group was 2.5 times of the control group after induced by adding 10% FCS in culture medium. CONCLUSION: atRA counteracts the effects of bFGF on the promotion of mitosis and inhibition of differentiation of NSCs. atRA also promotes NSCs to differentiate into neurons in vitro.     

应用热脉冲技术对小美旱杨耗水量的研究

应用热脉冲对河套灌区农田防护小美旱杨（Populus popular’s）日蒸腾耗水量进行了研究。结果表明：在正常生长状态下，小美旱杨的日蒸腾耗水量变化曲线呈宽峰状，随着林龄增加，日蒸腾耗水量逐渐增加，在不同水分条件下,灌水时日蒸腾耗水量：萌生苗日蒸腾耗水量大于扦插日蒸腾耗水量。     

踏郎沙地造林技术

文章介绍了内蒙古赤峰市沙地造林树种踏郎的造林技术     

中国矮马运铁蛋白遗传多态性的初步研究

采用聚丙烯酰胺凝胶电泳对中国矮马运铁蛋白多态性进行了测定。在运铁蛋白位点共发现5种表型，即F2F2，DF2，F2H2，F2O和F2R，由Tf^D,Tf^F2,Tf^F2,Tf^H2,Tf^O和Tf^R等5个等位基因控制，其基因频率分别为0.0312,0.7188,0.0625,0.0312和0.1563；基因的杂合度（H），个体鉴别概率（Dp)和亲仔关系排除概率（PE）分别为0.4530,0.6875和0.2586。     

血清8型鸭疫里墨氏杆菌在我国的发现及其病原特性研究

1994年1月-2000年10月，从全国23个省（市，自治区）不同代次（原种，祖代，父母代和商品代）的5-90日龄患有典型鸭传染性浆膜炎的病死鸭分离到68株血清8型的鸭疫里墨氏杆菌，对其病原学特性进行研究的结果表明，血清8型的鸭疫里墨氏杆菌在我国鸭群感染分布的范围极广，分离菌株对雏鸭具有很强的致病性，对小鼠无致病性，各地分离菌株表现出一致的形态特征和非常相似的生理生化特性；各地分离菌株耐药谱很广，但对头孢类药物（如头孢拉啶，头孢克洛等）和利福平却普遍高度敏感。用分离菌株制备的灭活疫苗免疫雏鸭具有良好的免疫原性。     

猪生殖与呼吸综合征病毒M基因和N基因的表达

将猪生殖与呼吸综合征病毒（PRRSV）的M基因和N基因从重组质粒pMD18-T—M—N中亚克隆至pBV220原核表达栽体上，成功构建了重组表达质粒pBVM—N。将pBVM—N转化大肠埃希氏菌DH5α感受态细胞．重组菌经温度敏感诱导表达，其细菌裂解物经SDS—PAGE可检测到分子质量约为19ku的目的蛋白，与M基因表达产物一致；经蛋白质分析软件Bandscan分析，其表达量可达19．1％；Western-blotting分析表明，该重组蛋白可被兔抗PRRSV血清所识别，与预期的M基因表达产物相一致，而N基因未获表达。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

Study on relationship between expression of PKCα in glomeruli and development of nephropathy in diabetic rats

AIM:To observe the dynamic changes of expression of PKCα, TGF-β₁ and α-SMA in glomeruli of diabetic rats induced by the alloxon and to invesitigate their roles in the diabetic nephropathy(DN).METHODS:Rats were randomly divided into four groups: normal control group (group A), diabetic group of one week (group B), diabetic group of one month (group C), diabetic group of two months (group D). Immunohistochemistry and Western blotting were used to detect the expression of PKCα, TGF-β₁ and α-SMA in renal tissue of all groups. Blood glucose, triglycerides, cholesterol, creatinine and urine protein were analysed by chemical methods. The morphological changes of renal tissue were checked through microscopy.RESULTS:The expression of PKCα and TGF-β₁ in renal tissue of diabetic groups were increased comparing with those of nomal control group(P<0.05). The mesangial cells expressed α-SMA in two months group. Chronologically the expression of PKCα, TGF-β₁ and α-SMA were positively correlative with each other and the impairment of kidney was also observed.CONCLUSIONS: During the DN process the expression of PKCα increased. PKCα raised GFR and the permeability of glomerular filtration membrane which enhanced urinary albumin excretion. PKCα also increased expression of TGF-β and therefore to induce the expression of α-SMA. The appearance of α-SMA was a marker of the phenotypic transform of renal cells.