牛头天社蛾初步观察

根据六六六泥团塞根有治螟效果的报导[黄山等,1956],为改进施药方法,我所于1957年进行土壤施药的盆栽药效測定,效果良好;1958年,田間防治試驗因处理时間太早(4月28日施药),效果不显;1960及1961年又进行田間試驗,因当年螟害輕微,防治效果也不够显著。据文献报导,六六六土壤施药,每亩用丙体六六六180克(折合数),对第一代二化螟防治效果良好,残效期达20—30天,并指出药剂系通过稻根内吸和沿叶鞘間上升的作用,杀死蛀入的蚁螟。昆明地区一般早栽田,栽秧后15—25天,正是螟卵盛孵期。1963年在昆明市郊区早栽田内,进行六六六土壤施药試驗,观察防治第一代三化螟的效果。試驗方法     

小麦对条锈病的水平抗病性研究初报

将小麦品种采用随机区组的田间设计种于小种病圃中,以鉴定和测定其水平抗性。根据各个“品种——小种”组合的相对病情指数的方差分析的结果,有些品种,如农大311、西农6028和丰产3号,其品种——小种互作高度显著,被鉴定为具有垂直抗性,另外一些品种,对试验所用的小种并无专化性,并且在大田生产中已显出20多年的持久的中度抗性,初步推测为属水平抗性。在后一类中,有些品种,如平原50,表现为中等反应型,其余则属于呈典型感病的迟锈慢锈类型。在迟锈慢锈品种的病指的对数矫正值(logits)和病圃的锈病强度(以感病对照品种的病指表之)的对数矫正值之间发现了直线回归。     

Decay of female sexual behavior under parthenogenesis

A laboratory strain of Drosophila mercatorum has existed for 20 years without males and therefore without natural selection operating to maintain the genetic basis of female mating behavior. The females of this strain have recently experienced a genetic impairment of mating capacity. This observation exemplifies the mode of evolution of vestigial characters and supports Muller's theory that random mutation will tend to destroy the genetic basis of a character from which selection has been removed.     

蛋白质组学研究技术及其在果树学中的应用

蛋白质组研究是当今生命科学发展的一个新的增长点,它能阐明基因组所表达的真正执行生命活动的全 部蛋白质的表达规律和生物功能。简要介绍了蛋白质组学产生的科学背景、研究方法和研究内容。蛋白质组学研究 方法主要有双向聚丙烯酰胺凝胶电泳(2D-PAGE)、质谱(Mass-spectrometric)技术、蛋白质芯片(Protein chips)技术、酵母 双杂交系统(Yeast two-hybrid system)、双向高效柱层析和生物信息学等。其应用的范围包括植物群体遗传学、在个体 水平上植物对生物和非生物环境的适应机制、植物的发育和组织器官的分化过程,以及不同亚细胞结构在生理生态 过程中的作用等诸多方面。同时展望了植物蛋白质组学研究前景以及蛋白质组学技术在果树学中的应用前景。     

猪生殖与呼吸综合征病毒M基因和N基因的表达

将猪生殖与呼吸综合征病毒（PRRSV）的M基因和N基因从重组质粒pMD18-T—M—N中亚克隆至pBV220原核表达栽体上，成功构建了重组表达质粒pBVM—N。将pBVM—N转化大肠埃希氏菌DH5α感受态细胞．重组菌经温度敏感诱导表达，其细菌裂解物经SDS—PAGE可检测到分子质量约为19ku的目的蛋白，与M基因表达产物一致；经蛋白质分析软件Bandscan分析，其表达量可达19．1％；Western-blotting分析表明，该重组蛋白可被兔抗PRRSV血清所识别，与预期的M基因表达产物相一致，而N基因未获表达。     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

花生在饲草生产中的间作套种技术与效果研究

本文研究了紫花苜蓿、白三叶、多年生黑麦草、鸡脚草和苇状羊茅等五种多年生牧 草与花生在“5+6”模式套作情况下生长特点以及对饲草生产的影响。花生与禾草的套作能够 极显著地促进禾草的分蘖(11．01％-14．26％)(P<0．01),显著增加其叶面积指数(4．62％- 6．37％),(P<0．05)三种禾草的产草量分别提高了8．2％、8．1％、9．3％。花生秧与禾草混合 调制后,饲草中的粗蛋白、粗脂肪和钙的含量得到了显著和极显著的提高,而粗纤维含量却明 显降低。紫花苜蓿和花生套作能够提高牧草产草量5．8％,豆科牧草与花生秧混合调制以后, 粗蛋白、粗纤维以及钙和磷的含量明显下降,粗脂肪得到了极显著的提高。     

论西部大开发中张掖市生态环境建设问题

张掖市长期受干旱、大风及沙尘暴影响,生态环境极为恶劣,制约着西部大开发建设进程。其存在的重要问题是:水资源匮乏,植被破坏严重,覆盖率低等;针对具体情况提出:改善水资源条件和恢复植被是张掖地区生态环境建设的重点。     

Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.