脐橙叶片矿质营养元素含量的分区分布特征

为揭示矿质营养元素在脐橙叶中分区分布特征,以枳砧和枳橙砧纽荷尔脐橙为对象,测定新叶和老叶的叶尖、叶缘、中部、叶基和翼叶等5个分区中10种元素(N、P、K、Ca、Mg、Fe、Mn、Cu、Zn和B)的含量,并分析各分区离子组成的差异。主成分分析显示,第一主成分(PC1)和第二主成分(PC2)分别解释了总变量的43.4%和21.1%,明确区分了新叶和老叶,大致区分了叶不同分区,发现新叶翼叶与其他分区具有明显差异,而老叶本叶各分区的离散程度明显大于新叶本叶各分区。两种砧木脐橙新叶中钾含量分布表现为翼叶叶基中部叶缘≈叶尖,镁含量表现为叶尖≈叶缘中部叶基翼叶;两种砧木脐橙老叶中锰含量表现为叶尖≈叶缘中部叶基翼叶,硼含量表现为叶尖叶缘中部叶基翼叶。N、P、B在新叶本叶各分区以及P、Ca在老叶本叶各分区间含量都没有显著差异。枳砧脐橙新叶翼叶Mn、Cu和Zn的含量与叶片其他分区没有显著差异,而枳橙砧脐橙新叶翼叶Mn、Cu和Zn的含量都分别显著低于叶片其他分区。结果表明,不同元素叶片分区分布特征存在明显差异,翼叶的元素含量和离子组成与叶片其他分区差异明显。     

Ethanol extract from Cortex Albiziae attenuates mouse acute liver injury induced by carbon tetrachloride via modulation of NF-κB pathway

AIM:To investigate the protective effect of ethanol extract from Cortex Albiziae on acute liver injury, and to explore its possible mechanism. METHODS:Acute liver injury in mice was induced by single intraperitoneal injection of 25% carbon tetrachloride (olive oil solubilization). The effective parts of ethanol extract from Cortex Albizziae against acute liver injury were screened. The pathological changes of the liver tissues were examined by pathological sections with HE staining. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) of the liver tissues were detected, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were mea-sured by ELISA, and the protein expression levels of NF-κB p65, Bcl-2 and Bax in the liver cells of the mice in each group were determined by Western blot. RESULTS:Compared with model group, the serum levels of AST and ALT in low-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-L, 4 mg·kg^-1·d^-1) group and high-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-H, 8 mg·kg^-1·d^-1) group were significantly decreased. The necrosis extent and degree of the hepatocytes and infiltration of inflammatory cells were significantly lower than that in model group. Compared with model group, the serum levels of TNF-α and IL-6 in AB-H group and AB-L group were significantly decreased (P<0.05). The protein level of NF-κB p65 in the nuclei of mouse liver cells in AB-H group and AB-L group were also decreased significantly (P<0.05). Compared with model group, the protein expression of Bax was decreased, the protein expression of Bcl-2 was increased, and the Bcl-2/Bax ratio was increased in AB-L group and AB-H group. CONCLUSION:The n-butanol phase of ethanol extract from Cortex Albiziae may protect the liver by reducing the activation of NF-κB p65, inhibiting the excessive release of inflammatory cytokines IL-6 and TNF-α, and decreasing hepatocyte apoptosis via regulating Bcl-2 and Bax expression.     

Metformin sensitizes human breast cancer MDA-MB-231 cells to tamo-xifen through down-regulation of c-Myc

AIM: To investigate whether metformin enhances the sensitivity of human breast cancer MDA-MB-231 cells to tamoxifen by down-regulating c-Myc. METHODS: The cell viability, colony formation, apoptosis, and migration and invasion abilities of MDA-MB-231 cells were detected by CCK-8 assay, colony formation experiment, flow cytometry and Transwell assay. The expression level of c-Myc was quantified by Western blot and immunohistochemical analysis. The antitumor effects of metformin and tamoxifen were investigated in vivo in a MDA-MB-231 triple-negative breast cancer xenograft model in the SCID mice. RESULTS: Metformin in combination with tamoxifen exerted synergistic effects on inhibition of the viability, colony formation, migration and invasion, and induced the apoptosis compared with the controls and either agent treatment alone in the MDA-MB-231 cells. The levels of c-Myc was down-regulated in vitro by treatment with metformin and/or tamoxifen (P<0.01). Moreover, metformin or in combination with tamoxifen also reduced the growth of MDA-MB-231 breast cancer tumors in the SCID mice by down-regulation of c-Myc in vivo. CONCLUSION: Metformin in combination with tamoxifen exerts synergistic effects on inhibition of the proliferation, migration, invasion and tumor growth of human triple-negative breast cancer MDA-MB-231 cells by down-regulating c-Myc expression, suggesting that metformin in combination with tamoxifen merits further evaluation as a target.     

探析林业低碳经济指标及统计

低碳经济作为一种新的发展模式,是人类社会继农业文明、工业文明之后的又一次重大进步.面对现今全球变暖的局面,怎样应用低碳经济指标,提高林业的统计质量就成为了人们越来越关注的事情.文章简要探讨了低碳经济的定义及指标,并提出相应的建议,目的在于进一步促进林业发展,保证人们的生存环境.     

中早熟高产抗病抗虫棉品种衡棉4号的选育

衡棉4号是用GK12为外源抗棉铃虫Bt基因的供体,以具有海岛棉、陆地棉和野生棉遗传基础的省1322系作抗病亲本,以冀棉27为丰产亲本,在黄枯萎混生重病地、不防治棉铃虫的高压胁迫下连续定向选择培育而成.突出表现早熟、抗棉铃虫、高产和抗病.适宜在冀中南及同一生态类型地区推广.     

不同剂量伊曲康唑制剂对犬血常规及肝肾功能的影响

旨在评价不同剂量伊曲康唑-环糊精制剂通过皮下注射途径对健康比格犬肝肾功能及血常规的影响.选用体重±10 kg的健康纯种比格犬32只,按试验要求随机分为4组,每组8只,建立稳定的生活制度.分别按照0.25 mg/kg、0.75 mg/kg和1.25 mg/kg剂量,皮下注射伊曲康唑制剂,3日1次,连续7次,对照组注射生理...     

剑麻可可毛色二孢叶斑病菌的鉴定及其室内药剂筛选试验

【目的】剑麻是我国重要的麻类作物,在广东湛江地区剑麻上发生一种叶斑病,导致剑麻叶片大面积坏死,严重影响产量。明确剑麻叶斑病的病原菌种类及防治药剂,以期对该病进行有效防治。【方法】采用组织分离法对病原菌进行分离,经科赫氏法则验证后得到病原菌的纯培养,结合真菌的形态特征和ITS序列分析对该病原菌进行鉴定,通过带毒介质菌丝生长速率法测定该病原真菌对不同药剂的敏感性。【结果】从广东湛江剑麻产区分离得到叶斑病致病菌,分生孢子椭圆形,单孢,深褐色,中间有一条中横隔隔开,形似双胞结合在一起,ITS序列比较分析表明其与可可毛色二孢菌（Lasiodiplodia theobromae）的同源性达到99%以上。百菌清、己唑醇、咪鲜胺、戊唑醇、苯醚甲环唑、氟吡菌酰胺和甲基硫菌灵等7种药剂对可可毛色二孢菌均具有较好的抑菌效果,其中百菌清抑菌效果最好,EC50值为0.000132μg/m L。【结论】广东湛江地区发生的剑麻叶斑病初步鉴定为由可可毛色二孢真菌引起,该病原菌对百菌清、己唑醇、咪鲜胺、戊唑醇、苯醚甲环唑、氟吡菌酰胺和甲基硫菌灵等药剂敏感。     

栽培措施对云科粳5号产量及品质的影响

【目的】探究栽培措施(移栽密度、施氮量)对优质粳稻品种云科粳5号产量及品质的影响。【方法】采用大田裂区设计,设置4个氮肥施用量(N 0、150、240、330 kg/hm²)和4个移栽密度(24万、30万、36万、42万苗/hm²)处理,测定稻谷产量性状、叶片SPAD值、糙米率、直链淀粉、精米率、垩白粒率、蛋白质、胶稠度等,探究施氮量和栽培密度对粳稻云科粳5号产量和品质的影响。【结果】在0～330 kg/hm²范围内,随着氮肥用量的增加,分蘖明显增加,高氮条件下个体分蘖数最高,达18.80个/穴;而随着移栽密度的增加,分蘖逐渐降低;产量随着移栽密度增加整体呈下降趋势,各施氮量处理下,移栽密度为24万苗/hm²获得最高产13 308 kg/hm²。云科粳5号在240 kg/hm²高氮水平下易取得高产,配合适宜的移栽密度能够进一步提高水稻产量;随着氮肥用量逐渐增加,垩白粒率、垩白度、胶稠度整体呈增加趋势,36万苗/hm²移栽密度在各施氮量...     

祁连山东缘高寒草地植物群落叶绿素高光谱反演模型的建立

祁连山高寒草地是我国北方草地的重要组成部分,近年来退化较严重,导致其产量、质量下降.利用ASD地物光谱仪采集了祁连山东缘高寒草地植物群落光谱数据,并利用Person相关系数法、PCA主成分分析法和VIP变量投影重要性法等方法,筛选了与高寒草地植物群落叶绿素相关性较高的原始光谱波段与植被指数,通过多元逐步回归与多元线性回...     

智能化沼气工程技术系统的推广价值研究

文章将智能化沼气工程与其他两种典型沼气工程进行经济效益比较,验证智能化工程沼气技术系统的优越性,主要研究结论为:智能控制系统与远程控制系统,减轻沼气工程设备及配件损耗,降低工程运行过程中的维修费用、管理费用与人工费用,提高技术服务机构或技术人员的管理效率与效果,保障沼气工程的产气效率及稳定性,为沼气发电和生产生活用能平稳供气,具有重要的推广价值。