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41.
42.
ZHOU Yan-bin YE Ren-gao XIE Can-mao XU Han-shi GUAN Wei-ming YANG Xiao YANG Nian-sheng 《园艺学报》2003,19(9):1221-1223
AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P<0.05;r=0.6279, P<0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN. 相似文献
43.
Bt对小地考虑呼吸作用的影响 总被引:5,自引:0,他引:5
利用红外CO2分析仪测定小地老虎幼虫呼吸节律的变化结果显示:小地老虎幼虫呼吸节律属于不规则波动型,取食含Bt烟叶的幼虫呼出的CO2量显著低于对照组,且呼吸节律的波动性也明显增大,说明小地考虑幼虫取食Bt后,呼吸代谢受到一定干扰,出现呼吸水平降低及代谢紊乱现象。 相似文献
44.
苹果紧凑型品种和矮化砧木内源激素的变化 总被引:15,自引:0,他引:15
以不同矮化程度的苹果品种、砧木和嫁接植株为试材, 研究了春梢旺长期茎尖和春梢停长期叶片的内源激素变化。结果表明, 与普通型品种相比, 紧凑型品种茎尖和叶片中的赤霉素(GA1+3) 含量显著降低而细胞分裂素(CTKs) 含量显著升高, 紧凑型品种的CTKs/ GA1+3约是普通型品种的4. 78~6. 24 倍。不同矮化程度的砧木间相比, 春梢停长期叶片中, M9 的IAA 含量比平邑甜茶降低了53. 7 %。以M26作中间砧, 显著降低了接穗叶片和基砧新根中的IAA 含量; 但以新红星作中间砧则影响较小。将M26中间砧与紧凑型品种组合, 与乔砧普通型植株相比, 春梢停长期叶片和新根中IAA 含量分别下降了46. 1 %和33. 3 %,CTKs/ GA1+3是其2. 8~3. 6 倍, 其变化幅度大于单一应用矮化砧、紧凑型品种的植株。因此, 紧凑型、矮化砧具有不同的激素调节机制, GA、CTK在紧凑型品种矮化中起重要作用, 而矮砧的矮化可能与IAA 密切相关。 相似文献
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46.
AIM:To investigate the effect of Se-containing spirulina phycocyanin (Se-SPC) on liver injury of mice induced by carbon tetrachloride (CCl4). METHODS:The mouse model was conducted by intragastric feeding with 2% CCl4 oil for three times, meanwhile Se-SPC, spirulina phycocyanin (SPC) and Na2SeO3 were injected (ip) to various groups for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD), alanine aminotransferase (ALT), malondiaoldehyde (MDA) and nitric oxide (NO) levels in blood and liver were measured. RESULTS:The level of Se,GPx and SOD activities were obviously higher(P<0.05)but ALT activity,MDA and NO2-/NO3- levels were remarkably lower(P<0.05)in Se-SPC treated groups than those in CCl4 groups,and effects of high dose Se-SPC on Se,GPx,MDA and NO2-/NO3- were even more significant(P<0.01).Under the same dose of Se or protein,effects of all selected targets in Se-SPC groups were more efficient than those in SPC groups and inorganic-Se groups.Furthermore,Se levels had a positive correlation with GPx activity(r=01705),which had negative correlation with levels of MDA,NO2-/NO3- and ALT(r=-0.629,r=-0.336,r=-0.457,respectively), and positive correlations between ALT activity and MDA or NO2-/NO3- level were found (r=0.519,r=0.641). CONCLUSION:These results indicated that Se-SPC may attenuate liver injury of mice induced by CCl4 through its anti-inflammatory action and enhancing selenoenzyme expression. 相似文献
47.
REN Yu-sheng WU Zong-gui CUI Fang JIA Guo-liang YU Shi-qing TANG Chao-wu LI Bo 《园艺学报》2002,18(11):1377-1380
AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [3H]-TdR incorporation and [3H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts. 相似文献
48.
AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer. 相似文献
49.
50.
利用RAPD标记评价桃种间杂交一代群体的分离方式 总被引:6,自引:2,他引:6
以毛桃2-7(Prunus persica)、山桃白山碧桃(Prunus davidiana)以及两者的F_1代为试材,对RAPD标记的分离方式进行分析。结果表明,RAPD标记在F_1代呈3种分离方式:孟德尔分离、偏离孟德尔分离规律、异常分离。3种分离位点出现的频率和数量分别为:80%、240,14%、42,6%、18。呈孟德尔分离的情况有:不分离、1:1分离和3:1分离,其中不分离的频率为64%。并对偏离孟德尔分离比例和异常分离的RAPD标记进行分析。其研究结果可为该群体是否适合构建遗传连锁图谱进行评价及进一步利用该群体奠定良好基础。 相似文献