湿地松×加勒比松F2子代苗高生长规律

湿加松F₂子代1年生实生苗的高生长节律可用Logistic曲线准确描述,苗高(Y)依生长天数(X)的生长模型Y=36.71/(1+53.93513e^-0.02971X),其回归和相关均达极显著水平,决定系数r²=0.9930,据此将苗高年生长过程划分为生长前期、生长高峰期、生长后期。分析了5-11月苗高净生长量与主要气象因子的关系。     

配平和求解化学反应方程式的数学模型

本文根据质量守恒定律建立了配平化学反应方程式的数学模型,并给出了模型的人工解法和计算机解法.该模型适合所有类型的化学反应,为数学模型构建提供了范例.     

秃杉种质资源迁地保存育苗技术研究

总结研究了13a来在地处亚热带在江苏省宁镇丘陵地区引种原产云贵高原、鄂西山地的珍稀树种秃杉获得在播种育苗与无性繁殖技术成果。该项 成果认为采用氧化剂消毒浸种侵芽,适时防治幼苗猝倒病,适度遮荫保湿,当年冬季防寒保苗等组装技术,可使每公斤种子产秃杉合格苗2．20万－5．60万株,1年生苗安全越冬;采用砻糠灰作基质,用KMnO4液浸条,在秋冬季扦插,生根率达41．3％－54．4％;用电子叶间隙喷雾,以珍珠岩作基质,或在日光温室内用塑料薄膜封闭保温,用KMnO4液浸条等技术,可使插条生根率达72．6％和76．1％。     

多胺对玉米种子吸胀期间耐冷性和种子发芽能力的影响

以玉米耐寒自交系黄C和低温敏感自交系Mo17为材料, 研究了亚精胺(Spd)和精胺(Spm)引发对玉米种子吸胀中的耐冷性和种子发芽能力的影响。种子经0.25 mmol L^-1的Spd和Spm引发72 h后, 在5℃下低温吸胀不同时间(12、24、36和48 h)。结果表明, Spd和Spm引发提高了胁迫期间两自交系胚内过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性, 以及可溶性蛋白质和脯氨酸(Pro)含量, 降低了胚渗出液的核苷酸含量, 同时显著提高发芽率, 并显著缩短平均发芽时间。低温吸胀48 h后, 与对照相比, Spd和Spm处理分别提高两自交系种子的平均发芽率18.5%和14.0%, 分别缩短平均发芽时间1.21 d和1.14 d。黄C在种子吸胀期间的耐冷性强于Mo17。总之, Spd和Spm处理能提高玉米种子吸胀期间的耐冷性, 提高低温胁迫下种子发芽能力。     

Exogenous carbon monoxide protects against the lung injury induced by ischemia-reperfusion of hind limbs in rats

AIM: To study the mechanism of protective effect of exogenous carbon monoxide (CO) in the lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. METHODS: Thirty-two SD rats were randomly divided into 4 groups: control, control+CO, IR and IR+CO. A rat model of ischemia in hind limbs and the reperfusion lung injury was made. The rats in IR+CO and control+CO groups were exposed to air containing 2.5×10-8 CO for 1 h before reperfusion or the corresponding control time point, while the other two groups were exposed to the routine air. The lung tissue structure, polymorphonuclear leukocyte (PMN) count, wet-to-dry weight ratio (W/D), malondialdehyde (MDA) content and the animal survival rate were observed. The carboxyhemoglobin (COHb) levels in artery blood were detected with CO-oximeter and the expression of intercellular adhesion molecule-1 (ICAM-1) in the lung was detected by Western blotting. RESULTS: Compared to control, the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly increased in IR group. Compared with the IR group, the blood COHb level was significantly increased and the animal mortality, lung PMNs number, W/D, MDA content and ICAM-1 expression were all significantly decreased in IR+CO group. CONCLUSION: These data suggest that exogenous CO attenuate limb IR-induced lung injury by down-regulatiny ICAM-1 expression and suppressing PMN sequestration in the lung following limb IR in rats.     

连续施用鸡粪对露地黄瓜产量、品质和土壤性质的影响

  【目的】  在连续4年施用不同量鸡粪条件下,探究露地黄瓜产量和品质的提升效果以及土壤性质的变化,以明确露地黄瓜生产中鸡粪的适宜用量。  【方法】  以黄瓜品种‘津优35号’为试材,2015—2018年在甘肃张掖进行了连续4年的田间定位试验,试验设两个土壤类型 (灌漠土和潮土)和5个鸡粪施用水平:不施用鸡粪 (对照,CK),15 t/hm2 (CM15)、30 t/hm2 (CM30)、45 t/hm2 (CM45) 和60 t/hm2 (CM60) ,采用随机区组试验设计。于2018年黄瓜结果盛期,采集黄瓜整株和0—20 cm耕层土壤样品,分析黄瓜产量、品质以及土壤容重、pH、有机质含量和酶 (脲酶、蔗糖酶、碱性磷酸酶和过氧化氢酶) 活性。  【结果】  与不施用鸡粪 (CK) 相比,CM15处理增产效果不显著,CM30、CM45和CM60处理黄瓜产量显著提高26.4%～33.0% (灌漠土) 和48.7%～50.1% (潮土),这3个处理之间无显著差异;施用鸡粪不同程度地提高了黄瓜可溶性固形物、可溶性蛋白质、可溶性糖和Vc含量,降低硝酸盐含量,效果以CM15、CM30处理较好。土壤容重和pH降幅随鸡粪施用量的增加而加大,有机质含量增幅随鸡粪施用量的增加而增加,持续施用鸡粪30～60 t/hm2可显著提高土壤有机质含量、降低土壤容重和pH。土壤脲酶、蔗糖酶、碱性磷酸酶以及过氧化氢酶活性以CM30、CM45处理较高。黄瓜产量与土壤容重和pH呈显著或极显著负相关,与有机质含量、脲酶、蔗糖酶和过氧化氢酶活性呈显著或极显著正相关;黄瓜可溶性固形物含量与容重呈显著负相关,可溶性糖含量与土壤pH呈显著负相关,硝酸盐含量与土壤pH呈显著正相关,与有机质含量呈显著负相关,可溶性固形物和Vc含量与土壤碱性磷酸酶活性呈显著正相关,可溶性蛋白质和可溶性糖含量与土壤蔗糖酶活性呈显著正相关。  【结论】  鸡粪施用处理降低土壤容重和pH,增加有机质含量和酶活性。鸡粪施用对土壤容重、pH、有机质和酶活性的改良效果与黄瓜产量效应值呈显著或极显著相关关系。黄瓜可溶性固形物与土壤容重,可溶性糖与土壤pH,硝酸盐与土壤pH和有机质密切相关。综合考虑鸡粪施用量在黄瓜产量、品质、改良土壤理化性状上的效果以及肥料效益,灌漠土和潮土均推荐施用30 t/hm2鸡粪。     

基于BP神经网络的三峡库区重庆段水资源安全评价

三峡库区水资源安全关系到库区的生态安全,对库区水资源安全利用进行评价,找出问题较为严重的区域,明确其限制因子,从而为库区水资源可持续发展和水资源安全提供科学的决策依据。结合2000—2014年三峡库区水资源安全数据,选取BP神经网络构建水资源安全利用评价模型,结合ARIMA模型进行指标预测,分析了三峡库区重庆段水资源安全利用的时空差异。研究表明:(1)三峡库区重庆段2014年水资源安全利用总体分布在较不安全到较安全之间,渝中区和大渡口区为较不安全等级,沙坪坝区等7个区县为基本安全,其他区县为较安全等级;在子系统的评价中,主城区的社会安全与供需安全等级均为最低,为极不安全和较不安全等级。(2)2000—2014年水资源安全利用等级主要受生态安全因子制约,供需安全因子次之;2015—2020年水资源安全利用等级主要受供需安全因子限制,生态安全因子次之。2000—2020年水资源安全利用等级总体呈上升趋势。     

小叶满江红内生细菌多样性的PCR—DGGE及电子显微镜分析

用16SrDNA-PCR-DGGE技术、电子显微镜和平板培养的方法系统地揭示了小叶满江红(Azolla microphylla)中内生细菌的遗传多样性和表型多样性.16S rDNA-PCR-DGGE的指纹图谱分析表明.在小叶满江红-蓝藻共生体内存在一个以蜡质芽胞杆菌(Bacillus cereus)为优势种群的复杂、多样的细菌区系;内生细菌在体内呈现各异的超微结构特征,体外培养生成不同大小、形态和色素的菌落.应用16S rDNA-PCR-DGGE技术能较全面地揭示满江红内生细菌的遗传多样性.     

不同抗热应激添加剂对热应激小鼠精液品质的影响

为探讨抗热应激添加剂对热应激状态下小鼠精液品质的影响,选取年龄和健康状况相近的成年雄性鼠150只,按照常温、热应激、添加剂处理后热应激的方式进行分组试验,对各组精液品质等指标进行统计分析。结果表明,3组热应激添加剂在缓解热应激小鼠的精液品质的影响效果上存在显著差异,其中效果最好的为中药组,其次是电解质组,最后是维生素组。从Hsp70mRNA表达量上来看,抗热应激添加剂组明显高于常温对照组。但与热应激组比较,维生素组与电解质组表达量低,而中药组表达量高。     

Role of translationally controlled tumor protein in PRL-3-promoted metastasis of colon cancer cells

AIM: To study the role of translationally controlled tumor protein (TCTP) in the proliferation, migration and invasion of phosphatase of regenerating liver-3(PRL-3)-promoted colon cancer cells.METHODS: The vectors pAcGFP-C3 and pAcGFP-C3-PRL-3 were constructed and transfected into the colon cancer cell line LoVo.LoVo-PRL-3 cells stably expressing PRL-3 and LoVo-control cells were established. The expression levels of PRL-3 and TCTP in both cells were detected by Western blotting and real-time PCR. The specific siRNA sequence for TCTP mRNA and control-siRNA were synthesized and transfected into the LoVo-PRL-3 cells. TCTP expression at mRNA and protein levels in LoVo-PRL-3 was detected by Western blotting and real-time PCR 24 h, 48 h and 72 h after transfection. The proliferation, migration and invasion abilities of LoVo-control cells, LoVo-PRL-3 cells, TCTP-siRNA and control-siRNA cells were detected by CCK-8 assay and the method of Transwell cell culture chambers.RESULTS: The expression of TCTP at mRNA and protein levels in LoVo cells was significantly increased after PRL-3 transfection (P<0.05). TCTP mRNA was significantly inhibited 24 h, 48 h and 72 h after transfection of TCTP-siRNA (P<0.01). TCTP protein was also significantly inhibited 48 h and 72 h after transfection (P<0.01). Compared with LoVo-control cells, the proliferation, migration and invasion abilities of LoVo-PRL-3 cells were significantly enhanced (P<0.05). However, lowering the up-regulated expression of TCTP in LoVo-PRL-3 cells inhibited the proliferation, migration and invasion abilities (P<0.05). CONCLUSION: PRL-3 promotes proliferation, migration and invasion of colon cancer cells by up-regulating the TCTP expression. siRNA targeting TCTP may be an effective method for prevention and treatment of colon cancer cell metastasis.