芍药栽培品种ISSR反应体系的优化和应用

以栽培芍药为材料,通过对ISSR-PCR反应体系中的Mg~(2 )、dNTP和Tag DNA聚合酶浓度的优化,以改进芍药的ISSR反应体系;从55对引物中筛选出4对有稳定扩增结果的引物;用这4对引物对22个芍药栽培品种的DNA进行扩增,其所得扩增产物与所选芍药性状经过数据处理后,用MATLAB软件分别进行聚类分析,结果表明供试芍药品种皆可分为3个类群,其分子标记与性状的聚类分析间有较强的相关性。     

利用家蚕表达合成人表皮生长因子(EGF)及产物对胃粘膜损伤的修复作用

基于家蚕杆状病毒密码子的偏爱性,对人表皮生长因子基因(hEGF)的部分密码子进行修改后合成一个新的基因,命名为CSEGF。所合成的CSEGF能编码同hEGF完全一致的氨基酸序列,并被重组进家蚕杆状病毒获得重组Bm-BacCSEGF。重组病毒感染家蚕后,该基因在家蚕中的表达量达28.4μg/mL幼虫血淋巴及33.07μg/mL蛹血淋巴。将表达CSEGF的蚕蛹经冻干直接制成EGF冻干粉胶囊,经测定冻干粉中EGF的质量比达140μg/g。动物模型试验显示该冻干粉对酸诱导的急性胃粘膜损伤的SD大鼠有显著的修复作用,对大鼠裸鼠的肾细胞生长也有明显的促进作用。     

鱼源气单胞菌的主要特性研究

对分离于草鱼肠炎、白鲢鱼打印病、鲢鱼白皮病、草鱼烂鳃、鲤鱼体表出血感染及甲鱼败血症等病例的80株细胞进行了主要特性研究。结果表明均为气单胞菌属（Aeromonas)的细菌，其中嗜水气单胞菌（A.hydrophila)29株（占36．25％）、豚鼠气单胞菌（A.caviae)38株（占47．5％）、温和气单胞菌A.sobria）13株（占16．25％）。在被检验的病例中，或为其中一种气单胞菌的单独感染，或为两种、三种气单胞菌的混合感染。经做人工感染试验检查，表明这些分离菌均为相应感染症的致病菌。     

工业园区绿地系统规划浅析——以淮安工业园区绿地系统规划为例

介绍了当前我国工业园区绿地系统规划的概念及意义,并以淮安工业园区为代表,探讨了工业园区绿地系统规划的相关内容,可为我国工业园区绿地系统规划提供一定的借鉴。     

学术期刊影响因子的应用研究

学术期刊影响因子已经由一种模糊的指标,成为衡量期刊、学术论文、作者、机构等学术研究水平高低的重要的定量测定指标。通过对学术期刊影响因子的使用进行了系统分析,提出影响因子的偏差的负面影响,对如何利用期刊影响因子公正有效的对成果鉴定进行了相关阐述。     

成都市郊区土壤芽孢杆菌的解磷、解钾潜力

采用稀释平板法对成都市郊区27个土壤样品进行了芽孢杆菌的分离,获得333个菌株。在解磷、解钾培养基上分析其解磷、解钾能力。结果表明,约有15%的土壤芽孢杆菌菌株分解无机磷的效果显著,分属15种芽孢杆菌,其中地衣芽孢杆菌(Bacillus licheniformis)、巨大芽孢杆菌(B.megaterium)和短小芽孢杆菌(B.pumilus)为分解无机磷的优势芽孢杆菌。多数菌株也能在有机磷和钾细菌培养基上生长,但分解能力较差。     

气候变化对内蒙古农牧业生态环境的影响

分析了数十年来气候变暖对内蒙古农牧业生态环境的影响。发现气候变暖使内蒙古地区无霜期延长,积雪、冰雹、雷暴、大风、沙尘暴日数减少,降水量也出现由少转多的变化。事实表明,气候增暖使内蒙古总的生态环境出现向好转化,但是增暖所带来的负面影响是病虫害的增加,加上人为的作用,严重地破坏了内蒙古的生态平衡。     

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.     

Effects of hypothalamic supraoptic ADH neurons at different times after hypophysectomy in Wistar rats

AIM:To observe the effects of supraoptic ADH neurons and central diabetes insipidus (CDI) in Wistar rats at different times after hypophysectomy.METHODS:Hypophysectomy was undergone by stereotaxic instrument.Water intake,urine output and urine specific gravity (SG) were observed each day.The survival rate of supraoptic ADH neurons was determined by immunofluorescence after hypophysectomy at different times.RESULTS:The rats manifested triphasic CDI after hypophysectomy.The average water intake in experiment group was 73.9 mL vs 30.9 mL in control group (P<0.01).Urine output was 59.7 mL vs 14.7 mL (P<0.01) and SG was 1.015 vs 1.036 (P<0.01).There was minor decrease in ADH neurons at the 3rd day after hypophysectomy (survival 94%,P>0.05),but the cellular body hypertrophy appeared.The survival rate at 10th day was 72％(P<0.01),31% at 20th day and 29% at 30th day.There was no difference between the 20th and 30th day (P>0.05),but they were all less than the 10th day (P<0.01).The ADH immunoreactivity enhanced in median eminence (ME) at the 20th day.CONCLUSION:The triphasic CDI appeard and supraoptic ADH neurons degenerated but the function of those survival neurons had compensatory enhancement after hypophysectomy.