Establishment and Application of Quantitative Real-time PCR Detection Method for Feline Infectious Rhinotracheitis Virus

Feline herpesvirus type 1 (FHV-1) is the pathogeny of feline infectious rhinotracheitis.Based on the sequence of gD gene of FHV-1, one pair of primers(FHV-1F and FHV-1R) and FHV-1Probe were designed for the quantitative Real-time PCR detection method.The results showed that the method could detect FHV-1 DNA specifically and the sensitivity was 75 fg/μL.The reaction efficiency reached 107%, and the R² value was 0.9965.The coefficient of variance(CV) was below 2%.And the method was more sensitive than common PCR.It was specific, stable, and suitable for clinical diagnosis, detection and epidemiological investigation.Using this method, the Shanghai area pet clinic censorship suspected FHV-1 infected samples were detected, and the positive rate reached 27.78%, which indicated that the incidence of FHV-1 in Shanghai area was at a high level.     

伊和乌素苏木1：10000地形图测绘首级平面控制网布设及质量控制

本文在面积较大、地形复杂、交通不便的沙漠地区,布设、施测了1:10000地形图测绘所需的I级GPS平面控制网.布网时把现代GPS测量方法和传统测量方法相结合,综合考虑各种因素,确保了控制网的可靠性;通过控制网优化设计、合理配置观测时段和使用适当的质量控制手段,在最不利的图形条件下,节省了工作量,达到了较理想的精度要求效果.     

粮食仓储管理探讨

通过储粮技术和管理措施的实施获得粮食储存效益的最大化,已成为粮食仓储企业的重要工作目标。该文介绍了粮食储存企业的主要工作措施,以提高储粮水平。     

钾肥对棉纤维发育过程中可溶性糖、蛋白含量及相关酶活性的影响

以陆地棉品种新陆早24号为材料,设5个施钾处理,进行田间小区试验,研究钾肥不同处理对开花后棉纤维发育过程中可溶性糖、蛋白含量及相关酶活性的影响。结果表明:施一定量钾肥(150 kg/hm2)可促进棉纤维的伸长发育,施钾后降低了棉纤维POD活性和IAA氧化酶的活性,抑制棉纤维的加厚并促进纤维的伸长发育;钾肥不同处理降低棉纤维发育中可溶性糖和可溶性蛋白质含量,促进棉纤维的发育。施用一定量的钾肥可以促进棉株对养分的吸收,影响棉纤维的生长发育。     

向大豆导入琉璃苣Δ6-脂肪酸脱氢酶基因的初步研究

采用RT-PCR法扩增出中国境内生长的琉璃苣Δ6-脂肪酸脱氢酶基因,序列分析表明,该基因全长为1 359个核苷酸,与美国德克萨斯州的琉璃苣Δ6-脂肪酸脱氢酶基因同源性可达到99%。将克隆到的基因连接pRTL2质粒的35S启动子,构建重组载体pPTN-D6D,并通过农杆菌介导的子叶节转化系统,将该基因导入到垦农18和小粒豆2个大豆品种中。经PCR检测分析,初步证明琉璃苣Δ6-脂肪酸脱氢酶基因已导入并整合到大豆基因组中。     

仿土鸡蛋的蛋品质分析及变化规律研究

通过对仿土蛋鸡20、24、30、34、40周龄所产鸡蛋进行检测,得出有关蛋品质和营养成分的各种数据.在对开产第一周及一个连产周期蛋品质进行的检测结果表明,刚开产的仿土鸡蛋蛋品质优于产蛋期平均水平;在整个产蛋期、开产第一周,一个连产周期内蛋品质指标会随着产蛋期的延长呈现相应的变化规律.     

多山梨醇酯对玉米秸秆体外发酵活力的影响

采用体外培养技术研究添加0.2%的多山梨醇酯20、40、60和80对玉米秸秆体外发酵活力的影响。结果表明:多山梨醇酯60和多山梨醇酯80能够显著提高玉米秸秆体外发酵24h的累积产气量。与对照组相比,多山梨醇酯60组和多山梨醇酯80组24h累积产气量分别提高5.68%(P<0.05)和9.02%(P<0.05)。添加多山梨醇酯对玉米秸秆的发酵模式没有显著影响,但添加多山梨醇酯60和多山梨醇酯80可增强玉米秸秆在体外的发酵强度。除多山梨醇酯20外,添加其它多山梨醇酯使玉米秸秆干物质降解率较对照组提高5.2~8.1%(P<0.05),有机物降解率有提高的趋势。在发酵24h结束时,添加多山梨醇酯40、60和80均使氨态氮浓度下降,还原糖和MCP升高,对pH和总挥发性脂肪酸无影响。其中,多山梨醇酯60和多山梨醇酯80组还原糖浓度分别比对照组提高7.54%(P<0.05)和7.26%(P<0.05)。多山梨醇酯80组MCP较对照组提高15.93%(P=0.061)。在体外培养条件下,多山梨醇酯60和80可促进玉米秸秆的降解。这说明,多山梨醇酯60和多山梨醇酯80可作为一种新型的反刍动物瘤胃发酵调控剂。     

高校试卷分析系统设计与实现

本文根据学生的试卷成绩进行试卷分析,软件可以算出考试成绩中的每个指标.并在Dephi中利用OLE技术实现打印功能.     

“中国园艺学会十届二次理事会暨学术研讨会”预备通知

AIM:The aim of the present study is to investigate changes of five biochemical markers of bone turnover during the formation of ovariectomized rat model. METHODS:Three-month-old female SD rats were divided randomly into ovariectomized (OVX) group, sham-operated (sham) group and control group. Five biochemical markers of bone turnover levels, including serum osteocalcin (OC), total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), tartrate-resistant acid phosphatase (TRAP) and hydroxyproline (HYP) were measured before and at 1, 1.5, 2, 2.5, 3 and 4 months after surgery. The right proximal tibias of rats were excised at the same time point for histopathological observation. RESULTS:Serum OC, ALP, BALP, TRAP and HYP levels in OVX group were significantly higher than that in sham group. As regard to the time of peak level arrived, the sequence of changes was as follows: TRAP/HYP→OC→ALP/BALP. There was a significant positive correlation between five markers. The pathological changes of trabecular bone in OVX group were only observed 3 months after surgery. CONCLUSION:The results suggest that postmenopausal osteoporosis has a high bone turnover rate. During the formation of ovariectomized rat model, changes of bone resorption markers precede changes of bone formation markers. Serum OC, ALP, BALP, TRAP and HYP are sensitive to evaluate the bone loss in the earlier stage of postmenopausal osteoporosis.     

轻简水肥一体化系统设计与实现

针对小规模农业灌溉应用中对水肥一体机高精度及低成本的需求,设计了一套轻简型水肥一体化系统.为实现精准水肥比例调控,系统可根据用户输入的标准水肥比和测量实时水速,利用脉宽调制技术通过改变占空比来调控肥速,直至满足水肥比误差要求.为降低测量误差的影响,设计中对传感器所测流量及水肥比例调控过程进行修正补偿,提高水肥调控精度.此外,提供了触摸屏和远程APP两种控制模式,操控方式简单,用户只需根据实际情况输入灌溉水肥总量,系统即可自动完成控制过程.结果表明,所设计的轻简水肥一体系统在不同水肥比例要求下,水肥配比精度高,运行稳定,可实现精准施肥灌溉.