中草药添加剂对生长肥育猪横纹肌肌纤维特性及肉质的影响

 选用20 kg左右的“杜长大”三元杂交猪80头,随机分成对照组、试验组,测定添加中草药添加剂对横纹肌肌纤维特性和肉质品质的影响。结果表明,试验组的大理石纹评分比对照组提高了23.33%（P＜0.01）、肌间脂肪的含量增加了52.77%（P＜0.01）、嫩度改善了13.53%（P＜0.05）;而失水率、贮存损失分别降低了5.03%（P＜0.05）和20.35%（P＜0.05）.试验猪的肌纤维直径比对照组降低了7.98%(P＜0.05)、而密度增加了4.70%(P＜0.05).结果提示,添加中草药添加剂对肉质的改善的作用与横纹肌肌纤维直径的减少、肌间脂肪含量的增加有关。     

传染性法氏囊病疫苗免疫种鸡后子代雏鸡的免疫学变化

应用现代免疫学新技术对传染性法氏囊病 (IBD)疫苗免疫种鸡后 ,其子代雏鸡外周血液和免疫器官组织的免疫学变化进行了动态研究。结果发现 :传染性法氏囊病疫苗免疫种鸡后 ,其子代雏鸡外周血液 T、B细胞数量和 Ig G,Ig M,Ig A含量及免疫器官组织的 T细胞、Ig G,Ig M,Ig A抗体生成细胞数量均不同程度地高于未免疫的相应对照雏鸡 ,表明 IBD疫苗免疫母鸡后 ,子代雏鸡外周血液和免疫器官组织的体液免疫和细胞免疫功能明显增强。而传染性法氏囊病超强毒攻击子代雏鸡后 ,未免疫的子代雏鸡 ,外周血液和免疫器官组织的上述各项指标均明显低于疫苗免疫的子代雏鸡 ,这与 IBDV感染雏鸡后 ,其免疫器官组织严重损害 ,淋巴细胞变性坏死等有关 ,也是导致感染雏鸡免疫抑制的基础     

Larval diet affects development and reproduction of East Asian strain of the fall armyworm,Spodoptera frugiperda

In December 11, 2018, the fall armyworm(FAW), Spodoptera frugiperda invaded China and has since impacted local maize, sorghum and other crops. Here, we draw on laboratory experiments to show how different host crops(i.e., maize, sorghum, wheat and rice) and artificial diet affect larval growth and adult reproduction of one local FAW strain. Larval diet affected development duration, pupation rate, survival and emergence rate of pupae, and S. frugiperda adult fecundity. FAW attained the slowest larval development(19.4 days) on sorghum and the fastest(14.1 days) on artificial diet, with larvae attaining 99.6% survival on the latter food item. On rice, FAW larvae attained survival rate of 0.4% and were unable to pupate successfully. Pupation rate and pupal survival varied substantially between artificial diet and live plantlets at different phenological stages. Pupal weight was the highest(0.26 g) on artificial diet and the lowest(0.14 g) on sorghum, while FAW females reached the highest fecundity(699.7 eggs/female) on 2-leaf stage maize. Egg hatching rate equaled 93.6% on 4-or 5-leaf stage maize and 36.6% on artificial diet. FAW intrinsic rate of natural increase and the finite rate of increase varied between larval diets, reflecting how young maize leaves are the most suitable diet. Our findings can help to refine laboratory rearing protocols, devise population forecasting models or guide the deployment of ‘area-wide' integrated pest management(IPM) modules in FAW-invaded areas of China and other Asian countries.     

辽东地区赤松落针病病原真菌的分类鉴定

对辽东主要地区发生落针病的赤松针叶上采集病害标本,室内进行分类鉴定,确定了在赤松针叶上有5种真菌,均是活体寄生。按照Minter（1981）分类标准进行了形态学种的分类鉴定,最后确定了5种真菌的分类地位,5个种分别为针叶树散斑壳菌、松散斑壳菌、光亮散斑壳菌、雪松散斑壳、小环绵盘菌。其中有3个种为我国赤松寄主上新记录种。     

在线接触式人造板厚度检测系统的设计

为了提升我国人造板生产质量,利用光栅尺位移传感器,设计一种在线接触式人造板厚度检测系统.系统基于Windows操作系统平台应用,可实现板材厚度参数设置、数据分析、检测记录、存储及实时显示数据等功能;同时,可与更高一级管理系统或TCP/IP以太网或H1总线进行数据通讯,为板材厚度纠偏传递信息.应用试验结果表明,系统运行平稳,在线重复性测量数据稳定、可靠.     

基于TM影像数据的林分冠层郁闭度反演技术研究

本文以崂山林场为研究区域,利用森林资源二类调查数据和TM影像数据,分析了林分郁闭度与遥感因子之间的定量关系,在此基础上利用多元回归分析法结合实测数据构建郁闭度估测模型,并对模型精度进行检验,结果表明,预估精度达到81.6%,估测效果较好。利用该模型,反演了研究区的林分冠层郁闭度,将崂山林场的林分冠层郁闭度分为四个等级,即非林地区,低郁闭度区,中郁闭度区和高郁闭度区,研究区的森林郁闭度分布呈现西北部和东南部较低,而中部和南部相对较高。     

我国蔬菜作物耐低温性研究进展

低温胁迫是蔬菜生产过程中的主要逆境因子,对蔬菜作物的生长发育、商品产量形成影响极大。本文综述了蔬菜作物耐低温性的遗传模型、生理调节和分子机制,展望了蔬菜作物耐低温性的研究前景。     

F-box domain is involved in regulating the proliferation of MCF-7 cells by FBXO39 protein

AIM: To investigate the effect of F-box domain on the regulation of MCF-7 cell proliferation by FBXO39 protein. METHODS: The effect of F-box domain on the localization of FBXO39 protein in the MCF-7 cells was investigated. MCF-7 cell cDNA library was used as the template resource. The full-length cDNA sequence of FBXO39 was amplified by PCR method and subcloned into eukaryotic expression vector pEGFP-C2. The pEGFP-FBXO39ΔF (F-box domain deletion mutation) plasmid was successfully constructed with the template resource of pEGFP-FBXO39 plasmid. The recombinant plasmids were transfected into the MCF-7 cells, and then the expression of FBXO39 and FBXO39ΔF were determined by Western blot. The cellular localization of FBXO39 and FBXO39ΔF were observed by confocal microscopy. The localization of endogenous FBXO39 in the MCF-7 cells was detected by immunofluorescence staining. In addition, MTT and EdU assays were used to measure the cell proliferation, flow cytometry was used to measure the cell cycle distribution, and immunohistochemical staining was used to observe the expression of FBXO39 in the breast cancer and para-carcinoma tissues. RESULTS: The eukaryotic expression vector pEGFP-FBXO39 and pEGFP-FBXO39ΔF were constructed successfully. F-box domain had no effect on the cell localization of FBXO39. FBXO39 promoted MCF-7 cell proliferation but FBXO39ΔF did not. FBXO39 was highly expressed in the breast cancer tissues. CONCLUSION: F-box domain had no effect on the cellular localization of FBXO39 protein. However, it plays an important role in the biological function of FBXO39. FBXO39 may be related to breast cancer tumorigenesis.     

甘蔗叶不同还田方式对土壤养分的影响

为减轻秸秆焚烧所带来的环境问题和充分利用甘蔗叶资源,在大田环境下动态监测甘蔗叶粉碎、焚烧和深埋3种还田方式对土壤养分的影响。结果表明:甘蔗叶粉碎还田和深埋还田有利于甘蔗叶中有机碳及氮、磷、钾养分的缓慢释放;甘蔗叶焚烧还田提高了土壤碱解氮含量5.6~20.9 mg/kg和速效磷含量0.8~4.0 mg/kg,促进土壤中氮磷向有效态转化,焚烧还田初期显著增加了土壤速效钾的含量12.3~18.3 mg/kg,但焚烧会损失部分甘蔗叶中碳氮元素,也造成环境污染。综合分析认为甘蔗叶粉碎还田是比较省工、方便的处理方式。     

中国2种橡胶树炭疽病菌对咪鲜胺敏感性比较

炭疽病是橡胶树上一种重要的叶部病害,主要病原有胶孢炭疽菌和尖孢炭疽菌。为了解中国各植胶区炭疽菌对咪鲜胺的敏感性,采用生长速率法室内测定来自海南、云南、广东和广西4省(区)22株橡胶树胶孢炭疽病菌和15株尖孢炭疽菌对咪鲜胺的敏感性。结果表明:供试的37株橡胶炭疽病菌菌株对咪鲜胺的EC_(50)值为0.022 0~0.272 1 mg/L,平均为(0.061 7±0.055 4)mg/L,EC_(50)最大值是最小值的12.5倍。其中89.2%参试菌株的EC_(50)值介于0~0.100 0 mg/L。根据成组t检验分析结果表明,目前中国4个植胶区的2种橡胶炭疽病菌对咪鲜胺敏感性无明显差异。除了来自海南和云南地区的尖孢炭疽菌有显著性差异外,其他来自不同省份的胶孢炭疽菌和尖孢炭疽菌对咪鲜胺敏感性均无明显差异。研究显示中国橡胶炭疽菌对咪鲜胺仍具有较高的敏感性,但菌株间敏感性有一定的差异,存在一定的潜在抗性风险。