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111.
将近90%以上的恶性卵巢癌发生于覆盖在卵巢表面的单层上皮细胞——卵巢表皮上皮(ovarian surface epithelium,OSE),将其称之为卵巢表皮癌。由于先前研究人员未找到合适的动物模型,卵巢表皮上皮细胞的培养方法在近期才得以确立。作者旨在于总结卵巢表皮上皮的生物学特性及其细胞培养方法和不同物种间卵巢表皮上皮特性的变化。 相似文献
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114.
甜瓜结实花初花节位QTL分析 总被引:2,自引:1,他引:2
以WI998为母本(纯雌株、厚皮网纹甜瓜品系),TopMark为父本(雄全同株纯合品系),配置杂交组合,利用单粒传得到171个株系的重组自交系(F2S4)群体构建甜瓜SSR分子标记遗传连锁图谱。该连锁图谱包含19个连锁群,覆盖基因组长度为1 414.2 cM,标记间平均距离为10.2 cM。对结实花初花节位开展QTL分析,共检测到9个QTL,分别分布在第1、9、10、11连锁群上,各QTL的LOD值在2.89 ~ 9.42之间,4个QTL贡献率超过10%。位于第9连锁群的QTL Fp9.4贡献率最大,为18.57%(2010秋季LOD = 9.17);位于第9连锁群的Fp9.3(2010春季8.64%,LOD = 6.44;2010秋季17.99%,LOD = 9.42),位于第10连锁群的Fp10.1(2010春季3.59%,LOD = 2.89;2010秋季6.20%,LOD = 3.43)在两季的位置都很稳定。获得与结实花初花节位紧密连锁(< 10 cM)的8个特异标记(SSR01737、MU141991、TJ105、GCM206、SSR04910、MU173563、NR52、MU146331),为进一步开展QTL精细定位提供参考。 相似文献
115.
施肥对热带雨林下种植砂仁土壤氮矿化和硝化作用的影响 总被引:7,自引:1,他引:7
采用封顶埋管法(Close-TopIncubation)研究了施肥后西双版纳热带雨林下种植砂仁土壤的氮素净氨化、净硝化和净矿化速率。结果表明,种植砂仁的热带雨林土壤有机氮的净氨化、硝化和矿化速率明显受外来无机氮的影响,施入铵态氮肥后,土壤中的氨化速率明显提高(P<0.01),由对照土壤的-0.66(mg·kg-1·30d-1)增加至施肥处理土壤的1.65(mg·kg-1·30d-1),但硝化速率和矿化速率却显著降低(P<0.01),分别由对照土壤的6.79(mg·kg-1·30d-1)和6.13(mg·kg-1·30d-1)减少至施肥处理土壤的2.60(mg·kg-1·30d-1)和4.25(mg·kg-1·30d-1),导致了土壤中NH4+-N的积累。 相似文献
116.
为了解秦皇岛昌黎县狐和貉源致病性大肠埃希菌耐药性及耐药基因的情况,以昌黎县分离的15株狐和貉源致病性大肠埃希菌为试验菌株,采用药敏纸片法对抗菌药物进行药敏试验,并对四环素类耐药基因tetA、tetB、tetC、tetD,氨基糖苷类耐药基因strA-strB、aadA1,磺胺类耐药基因sul1、sul2、sul3进行PCR检测。结果显示,15株大肠埃希菌分离菌株对土霉素、多西环素、头孢拉定、新霉素、青霉素、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶的耐药率均为100%;对阿莫西林、庆大霉素、头孢曲松、阿米卡星、大观霉素、链霉素、复方新诺明、氨苄西林、环丙沙星耐药率分别为93.33%、53.33%、33.33%、73.33%、86.66%、86.66%、86.66%、66.67%和66.67%。9种耐药基因tetA、tetB、tetC、tetD、strA-strB、aadA1、sul1、sul2、sul3的检出率分别为0、46.67%、26.67%、13.33%、40%、100%、53.33%、46.67%和53.33%,其中四环素类耐药基因以tetB的检出率最高,氨基糖苷类耐药基因以aadA1的检出率最高,磺胺类耐药基因的检出率相差不大。说明15株狐和貉源大肠埃希菌耐药谱广,耐药性高,耐药基因流行普遍。 相似文献
117.
优化施肥对春小麦产量、氮素利用及氮平衡的影响 总被引:3,自引:0,他引:3
2009 ~ 2010年,在宁夏引黄灌区分别以宁春11号和宁春16号小麦为供试作物,利用田间试验研究了优化施肥(OPT)和习惯施肥(CON)对春小麦产量、氮素吸收利用和土壤硝态氮累积的影响,表观评估了土壤—小麦体系氮素平衡.结果表明,相对于CK处理,OPT和CON都显著提高春小麦籽粒产量地上部生物量,并促进籽粒N和地上... 相似文献
118.
Xinnan Zheng Weizhe Xu Qi Ying Jiajun Ni Xiaoyuan Jia Yanrong Zhou Ting Ye Gongchu Li Kan Chen 《Marine drugs》2022,20(11)
Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy. 相似文献
119.
LIU Cui-cui GUO Xiao-hong CAO Guo-qing GAO Peng-fei PU Zhong-de NIU Jiao-yan ZHANG Qi LI Meng JIN Yu-shu LI Bu-gao 《中国畜牧兽医》2015,42(10):2726-2731
The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs. 相似文献
120.
GAO Qing SHI Jizhen YANG Zhichao LUO Sunlin ZHANG Siwei HUANG Shangzhen ZHANG Hailiang LI Junwei GUO Gang WANG Yachun 《中国畜牧兽医》2007,47(12):4016-4023
To investigate the influencing factors of locomotion score (LS) and its impacts on production performance and blood routine index in Holstein,body condition score (BCS),LS and DHI (dairy herd improvement) of 1 486 lactating cows from two dairy farms,and the blood routine indexes of 101 lactating cows from one farm in Beijing were measured.The fixed model was used to analyze the impacts of herd,parity and lactation stage on LS,and the impacts of LS and BCS on daily milk yield,somatic cell score,milk composition and blood routine indexes in dairy cows.The results showed that both parity and lactation stage had significant impacts on LS.The LS increased with the increase of parit,the LS increased by 0.71 from first lactation to fifth and above lactation.The LS of cow in lactation stage Ⅲ(100-199 d) and Ⅵ (after 350 d) were significantly higher than cow in stage Ⅱ (45-99 d)(P<0.05),and both were higher than 2.The LS had no significant impacts on daily milk yield,milk composition and somatic cell score (P>0.05).However,the milk yield of the cow with LS≥4 was the lowest,and a decrease of 2.29 kg was found compared with the cow with LS=1.Among the various blood routine indexes,LS had significant impacts on red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count (P<0.05),and had no significant impacts on white blood cell count (P>0.05).With the increase of LS,the red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count of cows decreased with different extents.There were some problems on the body immunity and hematopoiesis of cows with poor LS.In dairy farm,regular evaluation of LS could be used to find the management problems of feet and legs,which had a great significance to reduce the loss of production performance and early culling caused by hoof disease. 相似文献