Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

海洋灾害对渔业资源管理的影响及其对策

近年来,恶性捕捞严重,海洋渔业资源管理模式落后。同时,人类过度开发利用以及不可抗因素时有发生,使得海洋生态系统遭到破坏,渔业资源管理也面临着新的问题。对我国渔业资源管理现状以及非常态下渔业资源的恢复与开发利用等方面进行了综述,对非常态下渔业资源管理中存在的问题进行了阐述,并提出了对应的管理措施,最后对非常态下渔业资源管理制度发展建设进行了展望。     

设施韭菜安全生产关键技术集成

生产绿色、安全、放心韭菜，除了应用优质品种、实施绿色韭菜安全施肥技术外，推广绿色韭菜韭蛆防控新技术是关键。     

丽水市园林地被植物种类及造景探讨

结合丽水市园林绿地地被植物调查,对地被植物进行分类,并对地被植物在城市园林造景中的应用进行探讨。     

1～21日龄艾维茵肉鸡净能需要量研究

本试验在实测饲粮玉米和豆粕净能(NE)值的基础上,研究1～21日龄艾维茵肉鸡净能需要量.选用1日龄的艾维茵肉公鸡250只,随机分为5组,每组5个重复,每个重复10只鸡,饲粮净能水平分别为8.97、9.25、9.54、9.83、10.11 MJ/kg,考察不同净能水平对1～21日龄艾维茵肉鸡生长性能和氮代谢的影响.结果表...     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.     

基于模糊数学感官评价和混料设计的低度甜糯玉米酒原料配方优化

为了优化以鲜食甜糯玉米为主要原料制备甜糯玉米酒的原料配方。试验运用模糊数学原理,将甜糯玉米酒感官品质数学抽象量化,以量化后的感官品质为指标,通过混料试验设计,建立原料配方与感官品质之间的回归模型。结果表明:该模型的拟合程度好(R=0.984 4,P0.001),可用于预测和分析甜糯玉米酒的感官品质。经模型优化可得,感官品质评价为"好"的甜糯玉米酒原料配方为鲜食糯玉米(66%)、鲜食甜玉米(17%)和糯米(17%)。本研究酿造的甜糯玉米酒的酒精度仅为4.75%,远低于一般低度酒,符合现代人的饮酒需求。     

我国马铃薯主产区病毒病发生情况调查

为了解我国马铃薯病毒病发生情况,在马铃薯主产区黑龙江、内蒙古、甘肃、云南等马铃薯田采集了具有典型病毒病症状的样品、疑似样品和随机无症样品,试管苗和原原种为随机样品,共649份。应用DASELISA方法筛查6种马铃薯主要病毒:PVX、PVY、PVS、PLRV、PVM和PVA。结果表明:129份样品为阳性,PVY的检出率最高,为9.86%,PVS次之,为6.47%;试管苗PVS检出最多,为32份,原原种PVX检出最多,为10份,大田样品PVY病毒发生比例最高,为54份;有38份样品是由多种病毒复合侵染造成的,大田PVY+PVS复合侵染率最高,为2.93%,PVS+PVY+PLRV次之,为0.98%,试管苗PVS+PVM复合侵染率最高,为4.85%。通过历年的检测结果比较,试管苗中PVS病毒检出率最高,原原种中PVX和PLRV病毒成为危害最为严重的病害,大田样品中PVY检出率一直居高不下。     

磷脂酶D的抗逆特性及其活性检测研究

介绍了磷脂酶D(PLD)的研究现状及进展,包括其分类、来源和用途,详述了PLD的抗逆特性和相关作用机理及对PLD活性检测的最新方法,对PLD在科学研究中的发展前景进行了展望。     

早熟禾新品种农菁15的选育

为选育高产优质饲用和坪用草品种,将早熟禾优异风干种子经50Gy60 Co-γ射线辐射处理后,通过田间系统选择,结合生物产量、返青率、营养品质、抗病性及坪用性能等指标的检测,选育出农菁15早熟禾;该品种抗寒、耐盐碱、抗病性强、适应性广、饲用价值较高;与对照优异相比,具有青绿期长、草产量高、种子产量高等优点;在黑龙江高寒地区平均干草产量达3 832.6kg·hm-2,成熟期平均种子产量达510.2kg·hm-2;适宜在黑龙江全省推广种植。