食用菌文化在现代景观设计中的应用探索

回归现实生活、构建人文审美体系是现代景观设计的发展趋势.在现代景观设计过程中,对食用菌文化元素进行合理改造、运用,使景观富有文化内涵,呈现独特的艺术气息.通过分析食用菌文化在现代景观设计中的应用背景与价值,提出提取造型、创造生态场景和营造审美意境等设计建议.     

Survivin-2B induces apoptosis of human breast cancer cells

AIM：To explore the role of survivin-2B in the process of tumor cell apoptosis. METHODS：The survivin-2B gene was cloned into pcDNA3.1 vector and the recombinant plasmid pcDNA3.1-survivin-2B was obtained. Human breast cancer MCF7 cells were transfected with pcDNA3.1 and pcDNA3.1-survivin-2B using Lipofectamine 2000. The cell cycle was determined by propidium iodide staining, and the apoptosis was detected by annexin V/7-AAD staining 48 h after transfection. Meanwhile, tatal RNA was extrated and multiplex polymerase chain reaction based on GenomeLab GeXP Genetic Analysis System was performed to detect the expression of 21 tumor-related genes. RESULTS：Flow cytometry analysis indicated that over-expression of survivin-2B promoted the apoptosis and cell cycle arrest of MCF7 cells. Compared with control group, totally 10 differential expressed genes were related to the over-expressed survivin-2B, among which 2 were up-regulated and 8 were down-regulated. The expression of aldehyde dehydrogenase 4 family member A1 (ALDH4A1) was 48% down-regulated, and the expression of protein regulator of cytokinesis 1 (PRC1) was 1.08 folds up-regulated. CONCLUSION：Survivin-2B induces the expression changes of some tumor-related genes, which results in the apoptosis and G2/M arrest of MCF7 cells.     

浅谈木兰林区山野资源的保护与开发利用

木兰林区属冀北山区,环卫京津,林下蕴含丰富的山野资源。通过对山野资源现状的思考,指出木兰林管局山野资源存在提前采收,掠夺式经营、资源分散,资源利用率低,经济效益不高等几个问题。同时对木兰林区山野资源的保护与开发利用提出应全面调查、统一规划、合理开发,多方联动、区域治理,重新整合资源、提高科技创新能力,着力构筑技术创新平台、利用木兰林区纯天然的生态优势,全力打造品牌、更新观念,合理开发利用,保护野生果菜资源和加大野生果菜产品的研发力度。     

Effects of chlorine gas exposure on lung haemodynamic and respiratory function in intact and isolated perfused rabbit lungs

AIM:To investigate the effect of high concentration of chlorine gas(Cl₂) exposure on lung haemodynamic and respiratory function in intact and isolated perfused rabbit lungs (IPLs). METHODS:8 intact and 10 IPLs were exposed to Cl₂ at high concentration(50×10^-4)for 20 min, as measured group, 8 additional intact and 9 IPLs, which were similarly treated but not exposed to Cl₂, served as controls. The changes of lung weigh of IPL(△W)、pressure of pulmonary artery(Pa)and venous pressure(Pv)、airway pressure and tidal volume(TV) were continuously measured and recorded simultaneously. RESULTS: In IPL group: While the perfusing blood flow was kept constant (133.3 mL/min), and Pv did not change, following the exposure, the Pa increased slightly, then the lung weight were increased significantly and the TV decreased . Hematocrit of perfusate of EIPL and parameters of lung water increased also. In intact group: Pa increased slightly, respiratory rate accelerated immediately, and TV decreased. CONCLUSION:Although mean Pa increased continuously and slightly in both intact and IPL group following the exposure to high concentration of Cl₂, the primary cause of edema was most likely to alter pulmonary capillary permeability. The respiratory rate accelerated and TV decreased due to exposure to Cl₂ enhanced hypoxia of intact rabbits.     

洋葱苯丙氨酸解氨酶基因AcPAL2的克隆与表达分析

利用简并PCR技术和RACE技术克隆得到了一条洋葱的苯丙氨酸解氨酶(PAL)基因全长cDNA序列。该cDNA序列全长2349 bp,编码长685个氨基酸残基的多肽序列,命名为AcPAL2。Blast分析表明该序列与虎眼万年青Galtonia saundersiae、野蕉Musa balbisiana的相似性均较高。Real-time PCR表达及花青素含量分析表明,红皮洋葱该基因表达量最大,而黄皮和白皮洋葱表达量极低;在红皮洋葱中该基因在膨大初期大量表达,并迅速降低至一定程度后趋于相对平稳表达,且与花青素的积累过程相一致。     

南疆巴旦木测土配方施肥效果试验初报

为给南疆巴旦木合理施肥提供科学依据,以南疆巴旦木主栽品种为试材,采用"3414"试验方案中的部分设计方案,在新疆喀什莎车县就氮、磷、钾肥的不同用量与配比对巴旦木产量的影响情况进行了田间试验,并运用数学模型对试验结果进行了统计分析。结果表明:氮、磷、钾各因素对巴旦木增产影响的大小依次为N>P>K,与对照相比,处理Ⅷ(N3P2K2)的产量最高(8 496.90 kg.hm-2),增产比率最明显(71.13%),达到了极显著水平;获得最高产量的N、P2O5、K2O的施入量分别为472.05、206.55、37.95 kg.hm-2;获得当年产量的N、P2O5、K2O的施入量的参考值分别为354~531、232.4~348.6、23.7~35.56 kg.hm-2,其配比为1.00∶0.67∶0.08。     

降水和气温因子与造林成活率关系的调查与分析

对东丰县大阳林场2006～2010年连续5 a的造林成活率进行调查,分析其与降水和气温两个因子的关系,结果表明：在造林树种、密度、立地条件和苗木质量基本相同的前提下,年平均温度是影响造林成活率的最关键因子,此外,3～5份的温度对成活率亦有显著影响,低温常导致造林成活率降低。降水量在满足苗木成活的基本要求后,其作用不甚明...     

利用农杆菌介导法获得RNAi转基因枫香的研究

随着我国林业生态工程和退耕还林的开展，木材生产与需求之间的矛盾愈加严重，培育速生优质大径材的研究更加重要。基于LEAFY基因在植物生长发育中的特性，利用PCR和Gateway技术构建了含有LEAFY基因保守序列的RNA干涉植物表达载体LFY-RI，利用根瘤农杆菌Agrobacterium tumefaciems介导法获得转基因植株，利用RNA干涉技术抑制转基因植株中LEAFY基因的表达，从而获得枫香Liquidambar formosana不育转基因植株。并利用聚合酶链式反应（PCR）技术和PCR-Southern杂交技术检测了转基因植株中外源基因整合情况。共获得了5株枫香转基因植株，分子检测表明，其中4株为转基因阳性植株。由于转基因植株还比较幼嫩，对它们生长发育以及木材品质的变化尚处于在观察中。图5参12     

日粮不同锌水平对獭兔组织器官锌浓度的影响

本试验研究了日粮中不同锌水平对生长獭兔各组织器官锌浓度的影响,结果显示日粮锌水平与肝脏、肾脏、胰脏、被毛、血清锌浓度间呈强正相关(p<0.01);与脾脏、胫骨锌浓度间呈中等正相关(p<0.05).而与心脏、腿肌锌浓度间无相关关系(p>0.05).     

渠灌区灌溉管理信息化发展模式探讨

探讨了我国渠灌区灌溉管理信息化的发展模式,阐述了灌区进行灌溉管理信息化建设的意义和必要性,构想了灌区灌溉信息网络系统的建网模式和基本框架。