Hs1 pro-1双子叶表达载体构建和转化大豆研究初报

用从德国引进的抗线虫基因Hs1pro-1构建表达载体并转化大豆,以获得抗线虫转基因植株.提取克隆载体p1832,用Nco 酶切、Klenow补平和Sac 酶切,回收基因片段;提取表达载体pBI121,用Sma 和Sac 酶切,回收大片段;目的片段用T4DNA连接酶连接并转化E.coli,鉴定重组质粒;用基因枪轰击转化大豆品种早熟1号,获得18株再生苗,其中3株经PCR检测呈阳性,通过Southern杂交,证明Hs1pro-1基因已整合到其中2株大豆的基因组中.     

福建大洋人工草地放牧羊补饲矿物质添加剂效果观察

根据大洋人工草地牧草的矿物质元素的含量和放牧羊矿物质元素需要量配制含有8种元素的矿物质添加剂,对放牧的罗姆尼羊和四川麻羊进行补饲试验,结果表明,2种羊的平均日增重、Hb、TSP和血清中Co、P元素的含量试验组都显著高于对照组(P<0.05),四川麻羊全血GSH-PX试验组显著高于对照组,罗姆尼羊血清中Mn、K元素的含量试验组显著高于对照组(p<0.05),2种羊的RBC、PCV、血糖、CP、GSH-PX(罗姆尼羊),以及血液中Sc、Ca、Na、Mg,Mn(四川麻羊)试验组均高于对照组,但差异都不显著,(p>0.05)。     

Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae

Actinobacillus pleuropneumoniae is the aetiological agent of porcine pleuropneumonia, a highly contagious and often fatal disease. A candidate live vaccine strain, potentially capable of cross-serovar protection, was constructed by deleting the section of the apxIA gene coding for the C-terminal segment of ApxI toxin of the A. pleuropneumoniae serovar 10 reference strain (D13039) and inserting a chloramphenicol resistance gene cassette. The mutant strain (termed D13039A(-)Chl(r)) produced an approximately 48kDa protein corresponding to the N-terminus of the ApxI toxin, and exhibited no haemolytic activity and lower virulence in mice compared with the parental strain. The mutant was evaluated in a vaccination-challenge trial in which pigs were given two intra-nasal doses of the mutant at 14 days intervals and then challenged 14 days after the last vaccination with either A. pleuropneumoniae serovar 1 (4074) or serovar 2 (S1536) or serovar 10 (D13039) reference strains. The haemolysin neutralisation titres of the pre-challenge sera were significantly higher in the vaccinated pigs than in the unvaccinated pigs. The mortalities, clinical signs and lung lesion scores in the vaccinated pigs were significantly lower than those in the unvaccinated pigs for the serovar 1 challenge. A significantly lower lung lesion score was also observed in the vaccinated pigs, compared with unvaccinated pigs, for serovar 2 challenge. Our work suggests that the mutant strain offers potential as a live attenuated pleuropneumonia vaccine that can provide cross-serovar protection.     

Preparation of a dicyandiamide-contained novel insulation paper and an experiment on the thermal aging characteristics of relevant oil-paper insulations

In order to study the influence of dicyandiamide added into the insulation paper on the oil-paper insulation during the aging process.The insulation paper containing dicyandiamide and the insulation paper without any stabilizer are prepared. They are immersed in transformer oil separately and placed in the aging chamber with predefined temperature of 130℃ for 31 days to perform thermal aging experiment of oil-paper insulation. The specimens are periodically sampled and some parameters are measured like the degree of polymerization(DP), the breakdown voltage, the nitrogen content, the acidity of insulation oil, and UV-Vis spectrum. The results show that the insulation paper added with dicyandiamide has a distinct anti-aging and anti-breakdown effect and the DP is 58% higher than that of blank sample at the later stage of aging. In the aging process, decline of the nitrogen content of insulation paper is not serious. The oil-paper sample containing dicyandiamide has a far lower value of acidity than that of blank sample, besides the oil color is deeper. Dicyandiamide in the insulation paper improves not only the anti-aging and anti-breakdown properties, but also the performance of oil in the aging process.     

木荷和杉木人工林天然降水、穿透雨和树干茎流DOC和DON 浓度及动态(英文)

尽管溶解有机碳(DOC)和溶解有机氮(DON)在森林养分循环中的作用日渐为人们所关注,但对它们的浓度及动态,特别是对亚热带森林DOC 和DON 的研究甚少。本文于2002 年通过野外天然降水及亚热带木荷和杉木人工林(monoculture plantations of Schima superba and Cunninghamia lanceolata,15 年生)穿透雨和树干茎流各水样的收集及室内各水样中DOC、NO3 -N、NH4 -N 和总溶解有机氮(TDN)浓度的测- +定,其中DON 浓度通过TDN 与NO3 -N、NH4 -N 的浓度差值来计算,- +结果表明,天然降水DOC 和DON 浓度分别为1.7 和0.13 mg·L-1。木荷人工林穿透雨DOC 和DON 浓度分别为11.2 和0.24 mg·L-1,高于杉木人工林的DOC 和DON 浓度(10.3 和0.19 mg·L-1)。杉木人工林树干茎流DOC 和DON 浓度(分别为19.1 和0.66 mg·L-1)明显高于木荷人工林(分别为17.6 和0.48 mg·L-1)。天然降水DOC 浓度的月变化不明显,而DON 浓度在夏季和秋季较高。两林分穿透雨DON 浓度的月动态与树干茎流的十分相似,均在雨季开始时(3 月)浓度增大。两林分穿透雨DOC 浓度在2-4 月间较高,而树干茎流DOC 浓度在9-11 月间较高。图4表2 参24。     

抗病虫基因新资源:海洋绿藻孔石莼凝集素基因

植物凝集素(Lecin)具有抵抗植物病原菌和农业害虫的活性以及抗病毒活性,在植物保护上具有广阔的应用前景。从海洋绿藻孔石莼中分离到一种凝集素基因序列,为培育抗病虫作物新品种提供了新资源。     

In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f.

In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wilfordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg·L–1) and NAA (0.1 mg·L–1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg·L–1) and NAA (0.1 mg·L–1). This yielded a multiplication rate of 2.4 fo...     

Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits

Using primers designed according to the published sequence of rice OsCRY1a gene,we obtained part of the gene fragment by PCR and constructed an RNA interference expression vector with it.To down-regulate the expression level of the gene or lead to the loss-of-function of the gene,the vector was then introduced into rice via Agrobacterium-mediated transformation.Based on the performance of the transgenic plants,the functions of the gene were analyzed and deduced.The results indicated that suppressing the expression of the gene retarded flowering for 16 d in rice with the plant height and grain length significantly increasing whereas other important agronomic traits observed remained unchanged apparently.     

大杯香菇辐射新株系农艺性状的遗传变异分析

对11个大杯香菇(原菌株和辐射选育新株系)的7个农艺性状进行了遗传变异分析、相关性分析和主成分分析.结果表明:盖质量、菇朵质量、柄质量、盖宽、柄长变异系数较大,分别为45.14%,39.78%,37.66%,28.44%和26.41%;盖厚变异系数较小,为17.04%;柄直径变异系数较小,为8.78%.在相关性上,菇朵质量与柄质量、盖质量和盖宽呈极显著正相关,柄质量与柄长和盖质量呈极显著和显著正相关,盖质量与盖宽呈极显著正相关.主成分分析表明,前4个特征值在7个特征值中累计贡献率达98.70%,即前4个主成分对变异的贡献率达98.70%.在农艺性状选择上,首先对变异大的性状进行选择是极其重要的.在辐射选育新株系时,应注意选择大杯香菇盖质量和朵质量均较大的新株系.     

关于p-赋范空间中保距离的映射(0《p≤1)

研究了p-赋范空间中保距离1的映射以及非扩张映射.证明了:若p-赋范空间中的映射保2个或者3个距离,则为等距映射.