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51.
Ndung'u MW Kaoneka B Hassanali A Lwande W Hooper AM Tayman F Zerbe O Torto B 《Journal of agricultural and food chemistry》2004,52(16):5027-5031
The crude methanol extracts of the root barks of Turraea wakefieldii and Turraea floribunda were found to show mosquito larvicidal activity against third-instar larvae of Anopheles gambiae sensu stricto. Four new limonoids comprising a vilasininoid 1 and three havanensinoids 2-4 were isolated from the chloroform fractions of the methanol extracts of T. wakefieldii and T. floribunda, respectively. The structures of the compounds were elucidated by NMR spectroscopy. Compounds 1, 2, and 4 had LD50 values of 7.1, 4.0, and 3.6 ppm, respectively, and were more potent than azadirachtin, which had an LD50 value of 57.1 ppm when tested against larvae of A. gambiae. 相似文献
52.
53.
Doucet D Otter DE Gauthier SF Foegeding EA 《Journal of agricultural and food chemistry》2003,51(21):6300-6308
Extensive hydrolysis of whey protein isolate by Alcalase was shown to induce gelation mainly via hydrophobic interactions. The aim of this work was to characterize the peptides released in order to better understand this phenomenon. The apparent molecular mass distribution indicated that aggregates were formed by small molecular mass peptides (<2000 Da). One hundred and thirty peptides with various lengths were identified by reversed-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Alcalase was observed to have a high specificity for aromatic (Phe, Trp, and Tyr), acidic (Glu), sulfur-containing (Met), aliphatic (Leu and Ala), hydroxyl (Ser), and basic (Lys) residues. Most peptides had an average hydrophobicity of 1-1.5 kcal/residue and a net charge of 0 at the pH at which gelation occurred (6.0). Therefore, an intermolecular attractive force such as hydrophobic interaction suggests the formation of aggregates that further leads to the formation of a gel. 相似文献
54.
Heat stress adversely affects wheat production in many regions of the world and is particularly detrimental during reproductive
development. The objective of this study was to identify novel quantitative trait loci (QTL) associated with improved heat
tolerance in wheat (Triticum aestivum L.) and to confirm previous QTL results. To accomplish this, a recombinant inbred line (RIL) population was subjected to
a three-day 38°C daytime heat stress treatment during early grain-filling. At maturity, a heat susceptibility index (HSI)
was calculated from the reduction of three main spike yield components; kernel number, total kernel weight, and single kernel
weight. The HSI, as well as temperature depression (TD) of the main spike and main flag leaf during heat stress were used
as phenotypic measures of heat tolerance. QTL analysis identified 14 QTL for HSI, with individual QTL explaining from 4.5
to 19.3% of the phenotypic variance. Seven of these QTL co-localized for both TD and HSI. At all seven loci, the allele for
a cooler flag leaf or spike temperature (up to 0.81°C) was associated with greater heat tolerance, indicated by a lower HSI.
In a comparison to previous QTL results in a RIL population utilizing the same source of heat tolerance, seven genome regions
for heat tolerance were consistently detected across populations. The genetic effect of combining three of these QTL, located
on chromosomes 1B, 5A, and 6D, demonstrate the potential benefit of selecting for multiple heat tolerance alleles simultaneously.
The genome regions identified in this study serve as potential target regions for fine-mapping and development of molecular
markers for more rapid development of heat tolerant germplasm. 相似文献
55.
不同施钾量对冬小麦几个光合特性和产量的影响 总被引:6,自引:0,他引:6
1992~1994年,在滇中山原红壤上所作的田间试验中,冬小麦在3种NP施用水平下施用不同用量的钾肥.研究的初步结果表明,在配施NP肥的基础上,随着施钾量的增加,小麦叶片的气孔阻力减少,提高叶片胞间CO2浓度和叶绿素含量,增加净光合速率,籽粒产量也有不同程度的增加. 相似文献
56.
番茄疮痂病病原菌分类、抗性遗传和分子标记辅助选择进展 总被引:2,自引:0,他引:2
番茄疮痂病是世界上所有番茄种植地区主要的细菌性病害之一,该病能引起严重的产量损失和品质下降,几十年来一直受到育种界的关注。由于该病病原菌存在多个种和小种以及对该病的抗性呈数量性状遗传,因此通过育种途径来改良抗性进展缓慢。分子标记辅助选择为育种家筛选、聚合或累加对多个小种的抗性提供了一种工具。以前的综述对1997年以前在病害的发生、症状、危害、综合防治、遗传分析和育种等方面的研究进行了较为详尽的总结。近年来,在病原菌种与小种的鉴定、抗源的发掘、抗性遗传的分子定位和分子标记辅助选择方面的研究取得了很大进展,传统育种与分子生物学方法有机结合的策略也初见成效。本文将对这些进展进行总结,并对分子标记在番茄疮痂病抗性育种中的应用前景进行讨论。 相似文献
57.
Cui H Cruz-Correa M Giardiello FM Hutcheon DF Kafonek DR Brandenburg S Wu Y He X Powe NR Feinberg AP 《Science (New York, N.Y.)》2003,299(5613):1753-1755
Loss of imprinting (LOI), an epigenetic alteration affecting the insulin-like growth factor II gene (IGF2), is found in normal colonic mucosa of about 30% of colorectal cancer (CRC) patients, but it is found in only 10% of healthy individuals. In a pilot study to investigate the utility of LOI as a marker of CRC risk, we evaluated 172 patients at a colonoscopy clinic. The adjusted odds ratio for LOI in lymphocytes was 5.15 for patients with a positive family history [95% confidence interval (95% CI), 1.70 to 16.96; probability P = 0.002], 3.46 for patients with adenomas (95% CI, 1.14 to 11.37; P = 0.026), and 21.7 for patients with CRC (95% CI, 3.48 to 153.6; P = 0.0005). LOI can be assayed with a DNA-based blood test, and it may be a valuable predictive marker of an individual's risk for CRC. 相似文献
58.
Nojima S Schal C Webster FX Santangelo RG Roelofs WL 《Science (New York, N.Y.)》2005,307(5712):1104-1106
The sex pheromone of the German cockroach, Blattella germanica, has been characterized as gentisyl quinone isovalerate. This cockroach is a major cause of allergic disease and serves as a mechanical vector of pathogens, making it one of the most important residential and food-associated pests worldwide. The sex pheromone-producing gland in adult females was identified in 1993, but thermal instability of the pheromone made characterization difficult. Now, using a new preparative gas chromatography approach coupled with electroantennographic detection, we have isolated and characterized the pheromone, which we term blattellaquinone, and confirmed the identification by chemical synthesis. The synthetic pheromone was active in behavioral assays and highly effective in field trapping tests, which suggest that it may provide a new tool in cockroach population detection, monitoring, and control. 相似文献
59.
Falush D Wirth T Linz B Pritchard JK Stephens M Kidd M Blaser MJ Graham DY Vacher S Perez-Perez GI Yamaoka Y Mégraud F Otto K Reichard U Katzowitsch E Wang X Achtman M Suerbaum S 《Science (New York, N.Y.)》2003,299(5612):1582-1585
Helicobacter pylori, a chronic gastric pathogen of human beings, can be divided into seven populations and subpopulations with distinct geographical distributions. These modern populations derive their gene pools from ancestral populations that arose in Africa, Central Asia, and East Asia. Subsequent spread can be attributed to human migratory fluxes such as the prehistoric colonization of Polynesia and the Americas, the neolithic introduction of farming to Europe, the Bantu expansion within Africa, and the slave trade. 相似文献
60.