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81.
82.
Marion D. Francis Candice L. Slough Hugh E. Black Andrew J. Tofe G. Gilbert Cloyd 《Veterinary radiology & ultrasound》1980,21(4):168-176
The following sequence of treatments was administered to a Saint Bernard dog with a primary distal right radius osteosarcoma: 54 days of daily disodium 1-hydroxyethylidenediphosphonate (HEDP) subcutaneous injections; 53 days of HEDP per os ; one 32 P-HEDP intravenous injection. During the pretreatment period, there was an extensive increase in calcific tumor growth and osteoblastic proliferation. After the subcutaneous HEDP treatment, almost complete tumor necrosis was seen. After the oral HEDP treatment, only the deepest tumor portion contained active osteoblasts, calcific growth of the tumor was completely blocked, and uptake of 99m Tc-Sn-HEDP was reduced to one fourth of the pretreatment uptake. After a single 32 P-HEDP dose, large areas of tumor necrosis were evident histopathologically. However, subsequent resumption of cellular activity occurred in the tumor, and the uptake of 99m Tc-Sn-HEDP increased to pretreatment values. These data suggest that systemically administered HEDP should be studied further for its possible therapeutic potential in the treatment of osteosarcoma and indicate a need for further study of 32 P-HEDP or possibly 33 P-HEDP. 相似文献
83.
Wakshlag JJ Cooper BJ Wakshlag RR Kallfelz FA Barr SC Nydam DV Dimauro S 《American journal of veterinary research》2004,65(4):480-484
OBJECTIVE: To perform respiratory chain enzymatic activity assays on canine skeletal muscle biopsy specimens and establish reference range values of skeletal muscle enzyme activities for dogs. SAMPLE POPULATION: Biopsy specimens from the vastus lateralis muscle were obtained from 24 dogs (8 sexually intact males and 14 sexually intact females) ranging from 15 months to 6 years of age. PROCEDURE: Mean values of citrate synthase, cytochrome-c oxidase, succinate dehydrogenase, succinate dehydrogenase-cytochrome-c reductase, nicotinamide adenine dinucleotide (NADH) dehydrogenase, and NADH dehydrogenase-cytochrome-c reductase activities were established by use of 6 standard spectrophotometric assays for respiratory chain enzyme analysis. RESULTS: Compared with published data for skeletal muscle enzyme activities in humans, skeletal muscle enzyme activities in dogs were 2- to 4-fold higher. Additionally, citrate synthase activity, a marker for mitochondrial volume, was positively correlated with age in dogs, suggesting that mitochondrial volume increases with age, although no apparent change in respiratory chain enzymatic activity with an increase in age was found. CONCLUSIONS AND CLINICAL RELEVANCE: Reference range values for skeletal muscle enzyme activities of dogs are needed to accurately interpret results of respiratory chain enzymatic activity assays. During investigation of metabolic myopathies, if skeletal muscle biopsy specimens are evaluated for respiratory chain enzyme kinetics, they should be performed and evaluated in concert with skeletal muscle biopsy specimens from clinically normal animals of the same species. 相似文献
84.
85.
A simple trapping procedure for concentration of volatile organic compounds present in a head space and the subsequent analysis of the retained compounds by combined gas chromatographymass spectrometry is described. The procedure was used to study the organic metabolites evolved from various soils when they were amended with glucose and incubated under argon. A number of organic acids, alcohols, aldehydes and esters were evolved during a 4 week period. Less than 10 per cent of the added carbon was recovered as organic compounds, which was largely due to poor recovery of volatile organic acids from soil. Some implications of the observations to microbial ecology are discussed. 相似文献
86.
Jon T. Biermacher Francis M. Epplin B. Wade Brorsen John B. Solie William R. Raun 《Precision Agriculture》2006,7(3):193-204
Research is ongoing to develop sensor-based systems to determine crop nitrogen needs. To be economic and to achieve wide adoption, a sensor-based site-specific application system must be sufficiently efficient to overcome both the cost disadvantage of dry and liquid sources of nitrogen relative to applications before planting of anhydrous ammonia and possible losses if weather prevents applications during the growing season. The objective of this study is to determine the expected maximum benefit of a precision N application system for winter wheat that senses and applies N to the growing crop in the spring relative to a uniform system that applies N before planting. An estimate of the maximum benefit would be useful to provide researchers with an upper bound on the cost of delivering an economically viable precision technology. Sixty five site-years of data from two dryland winter wheat nitrogen fertility experiments at experimental stations in the Southern Plains of the U.S.A. were used to estimate the expected returns from both a conventional uniform rate anhydrous ammonia (NH3) application system before planting and a precise topdressing system to determine the value of the latter. For prices of $0.55 and $0.33 kg−1 N for urea-ammonium nitrate (UAN) and NH3, respectively, the maximum net value of a system of precise sensor-based nitrogen application for winter wheat was about $22–$31 ha−1 depending upon location and assumptions regarding the existence of a plateau. However, for prices of $1.10 and $0.66 kg−1 N for UAN and NH3, respectively, the value was approximately $33 ha−1. The benefit of precise N application is sensitive to both the absolute and relative prices of UAN and NH3.This is journal paper AEJ-260 of the Oklahoma Agricultural Experiment Station, project H-2574. 相似文献
87.
Edery P Marcaillou C Sahbatou M Labalme A Chastang J Touraine R Tubacher E Senni F Bober MB Nampoothiri S Jouk PS Steichen E Berland S Toutain A Wise CA Sanlaville D Rousseau F Clerget-Darpoux F Leutenegger AL 《Science (New York, N.Y.)》2011,332(6026):240-243
The spliceosome, a ribonucleoprotein complex that includes proteins and small nuclear RNAs (snRNAs), catalyzes RNA splicing through intron excision and exon ligation to produce mature messenger RNAs, which, in turn serve as templates for protein translation. We identified four point mutations in the U4atac snRNA component of the minor spliceosome in patients with brain and bone malformations and unexplained postnatal death [microcephalic osteodysplastic primordial dwarfism type 1 (MOPD 1) or Taybi-Linder syndrome (TALS); Mendelian Inheritance in Man ID no. 210710]. Expression of a subgroup of genes, possibly linked to the disease phenotype, and minor intron splicing were affected in cell lines derived from TALS patients. Our findings demonstrate a crucial role of the minor spliceosome component U4atac snRNA in early human development and postnatal survival. 相似文献
88.
Nojima S Schal C Webster FX Santangelo RG Roelofs WL 《Science (New York, N.Y.)》2005,307(5712):1104-1106
The sex pheromone of the German cockroach, Blattella germanica, has been characterized as gentisyl quinone isovalerate. This cockroach is a major cause of allergic disease and serves as a mechanical vector of pathogens, making it one of the most important residential and food-associated pests worldwide. The sex pheromone-producing gland in adult females was identified in 1993, but thermal instability of the pheromone made characterization difficult. Now, using a new preparative gas chromatography approach coupled with electroantennographic detection, we have isolated and characterized the pheromone, which we term blattellaquinone, and confirmed the identification by chemical synthesis. The synthetic pheromone was active in behavioral assays and highly effective in field trapping tests, which suggest that it may provide a new tool in cockroach population detection, monitoring, and control. 相似文献
89.
Murphy WJ Larkin DM Everts-van der Wind A Bourque G Tesler G Auvil L Beever JE Chowdhary BP Galibert F Gatzke L Hitte C Meyers SN Milan D Ostrander EA Pape G Parker HG Raudsepp T Rogatcheva MB Schook LB Skow LC Welge M Womack JE O'brien SJ Pevzner PA Lewin HA 《Science (New York, N.Y.)》2005,309(5734):613-617
The genome organizations of eight phylogenetically distinct species from five mammalian orders were compared in order to address fundamental questions relating to mammalian chromosomal evolution. Rates of chromosome evolution within mammalian orders were found to increase since the Cretaceous-Tertiary boundary. Nearly 20% of chromosome breakpoint regions were reused during mammalian evolution; these reuse sites are also enriched for centromeres. Analysis of gene content in and around evolutionary breakpoint regions revealed increased gene density relative to the genome-wide average. We found that segmental duplications populate the majority of primate-specific breakpoints and often flank inverted chromosome segments, implicating their role in chromosomal rearrangement. 相似文献
90.
Cui H Cruz-Correa M Giardiello FM Hutcheon DF Kafonek DR Brandenburg S Wu Y He X Powe NR Feinberg AP 《Science (New York, N.Y.)》2003,299(5613):1753-1755
Loss of imprinting (LOI), an epigenetic alteration affecting the insulin-like growth factor II gene (IGF2), is found in normal colonic mucosa of about 30% of colorectal cancer (CRC) patients, but it is found in only 10% of healthy individuals. In a pilot study to investigate the utility of LOI as a marker of CRC risk, we evaluated 172 patients at a colonoscopy clinic. The adjusted odds ratio for LOI in lymphocytes was 5.15 for patients with a positive family history [95% confidence interval (95% CI), 1.70 to 16.96; probability P = 0.002], 3.46 for patients with adenomas (95% CI, 1.14 to 11.37; P = 0.026), and 21.7 for patients with CRC (95% CI, 3.48 to 153.6; P = 0.0005). LOI can be assayed with a DNA-based blood test, and it may be a valuable predictive marker of an individual's risk for CRC. 相似文献