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排序方式: 共有139条查询结果,搜索用时 15 毫秒
111.
Van Parys A Boyen F Verbrugghe E Leyman B Bram F Haesebrouck F Pasmans F 《Veterinary research》2012,43(1):52
ABSTRACT: Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. Salmonella Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Circumvention of the host's immune system by Salmonella might contribute to persistent infection of pigs. In the present study, we found that Salmonella Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine alveolar macrophages in a Salmonella pathogenicity island (SPI)-1 and SPI-2 dependent way. Salmonella induced downregulation of MHC II expression and intracellular proliferation of Salmonella in macrophages were significantly impaired after opsonization with Salmonella specific antibodies prior to inoculation. Furthermore, the capacity to downregulate MHC II expression on macrophages differed significantly among Salmonella strains, independently of strain specific differences in invasion capacity, Salmonella induced cytotoxicity and altered macrophage activation status. The fact that strain specific differences in MHC II downregulation did not correlate with the extent of in vitro SPI-1 or SPI-2 gene expression indicates that other factors are involved in MHC II downregulation as well. Since Salmonella strain dependent interference with the pig's immune response through downregulation of MHC II expression might indicate that certain Salmonella strains are more likely to escape serological detection, our findings are of major interest for Salmonella monitoring programs primarily based on serology. 相似文献
112.
Du Laing Gijs Meers Erik Dewispelaere Marjan Rinklebe Jörg Vandecasteele Bart Verloo Marc G. Tack Filip M. G. 《Water, air, and soil pollution》2009,199(1-4):353-369
Water, Air, & Soil Pollution - Many management strategies to improve the health of Chesapeake Bay focus on reducing losses of sediments and nutrients from agricultural land. Plot-scale studies... 相似文献
113.
Moore B Zhou L Rolland F Hall Q Cheng WH Liu YX Hwang I Jones T Sheen J 《Science (New York, N.Y.)》2003,300(5617):332-336
Glucose modulates many vital processes in photosynthetic plants. Analyses of Arabidopsis glucose insensitive2 (gin2) mutants define the physiological functions of a specific hexokinase (HXK1) in the plant glucose-signaling network. HXK1 coordinates intrinsic signals with extrinsic light intensity. HXK1 mutants lacking catalytic activity still support various signaling functions in gene expression, cell proliferation, root and inflorescence growth, and leaf expansion and senescence, thus demonstrating the uncoupling of glucose signaling from glucose metabolism. The gin2 mutants are also insensitive to auxin and hypersensitive to cytokinin. Plants use HXK as a glucose sensor to interrelate nutrient, light, and hormone signaling networks for controlling growth and development in response to the changing environment. 相似文献
114.
Alexander Van Parys Filip Boyen Bregje Leyman Elin Verbrugghe Dominiek Maes Freddy Haesebrouck Frank Pasmans 《Comparative immunology, microbiology and infectious diseases》2013
Foodborne salmonellosis is one of the most important bacterial zoonotic diseases worldwide. Salmonella Typhimurium is the serovar most frequently isolated from persistently infected slaughter pigs in Europe. Salmonella Typhimurium pathogenesis is host species specific. In addition, differences in in vitro behaviour of Salmonella Typhimurium strains have also been described, which may be reflected by a different course of infection within a host species. We compared the course of a Salmonella Typhimurium infection in pigs, using two Salmonella Typhimurium strains that were able to interfere with MHC II expression on porcine macrophages to a different extent in vitro. After experimental inoculation, blood and faecal samples from all pigs were collected at regular time points. At 40 days post inoculation (pi), animals were euthanized and tissue samples were bacteriologically analysed. The proportion of serologically positive piglets at 33 days pi was significantly higher in pigs that were inoculated with the strain that did not downregulate MHC II expression in vitro. Furthermore, this strain was less frequently shed and isolated in lower numbers from tonsils and ileocaecal lymph nodes than the strain that was able to markedly downregulate MHC II expression in vitro. We thus found that the delayed onset of seroconversion after oral inoculation of piglets with a particular Salmonella Typhimurium strain coincided with higher faecal shedding and increased persistence. Strain specific differences in Salmonella pathogenesis might thus have repercussions on the serological detection of Salmonella Typhimurium infections in pigs. 相似文献
115.
Gunther Antonissen Siska Croubels Frank Pasmans Richard Ducatelle Venessa Eeckhaut Mathias Devreese Marc Verlinden Freddy Haesebrouck Mia Eeckhout Sarah De Saeger Birgit Antlinger Barbara Novak An Martel Filip Van Immerseel 《Veterinary research》2015,46(1)
Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-015-0234-8) contains supplementary material, which is available to authorized users. 相似文献116.
Hermans D Van Deun K Martel A Van Immerseel F Messens W Heyndrickx M Haesebrouck F Pasmans F 《Veterinary research》2011,42(1):82
ABSTRACT: Campylobacter contaminated broiler chicken meat is an important source of foodborne gastroenteritis and poses a serious health burden in industrialized countries. Broiler chickens are commonly regarded as a natural host for this zoonotic pathogen and infected birds carry a very high C. jejuni load in their gastrointestinal tract, especially the ceca. This eventually results in contaminated carcasses during processing. Current intervention methods fail to reduce the colonization of broiler chicks by C. jejuni due to an incomplete understanding on the interaction between C. jejuni and its avian host. Clearly, C. jejuni developed several survival and colonization mechanisms which are responsible for its highly adapted nature to the chicken host. But how these mechanisms interact with one another, leading to persistent, high-level cecal colonization remains largely obscure. A plethora of mutagenesis studies in the past few years resulted in the identification of several of the genes and proteins of C. jejuni involved in different aspects of the cellular response of this bacterium in the chicken gut. In this review, a thorough, up-to-date overview will be given of the survival mechanisms and colonization factors of C. jejuni identified to date. These factors may contribute to our understanding on how C. jejuni survival and colonization in chicks is mediated, as well as provide potential targets for effective subunit vaccine development. 相似文献
117.
Effect of time and temperature on anticoagulant‐dependent pseudothrombocytopenia in Göttingen minipigs 下载免费PDF全文
118.
Van Immerseel F De Buck J De Smet I Pasmans F Haesebrouck F Ducatelle R 《Avian diseases》2004,48(2):384-391
In vitro studies of the interaction between pathogenic bacteria and the chicken intestinal epithelium are hampered by the lack of a host- and tissue-specific in vitro model. Therefore, a reproducible method for isolation and cultivation of chicken primary cecal epithelial cells was developed. Cecal crypts were isolated and cultured in vitro to form a semiconfluent layer of epithelial cells. Incubation of Salmonella enteritidis with these cells resulted in invasion. Pretreatment of the Salmonella bacteria with butyric acid resulted in a significant decrease of invasion of the bacteria in the chicken cecal epithelial cells, whereas pretreatment with acetic acid increased invasiveness. These interactions of S. enteritidis with primary chicken cecal epithelial cells were similar to the interactions with other epithelial cell types. 相似文献
119.
Results of intensive monitoring of forest floor decomposition rates and soil forming processes after afforestation of a calcareous upland dredged sediment landfill with an oxidised surface soil are presented. Nutrient status of the sediment substrate favours tree growth and allows for afforestation and thus an integration in the landscape. Soil processes on the landfill resulted in small differences between the surface and the subsurface soil layer, although higher soil organic carbon and Cd concentrations in the surface soil were observed. So far, tree species had a minor impact on soil processes. Relative to the uncontaminated covered part of the site and to general references, forest floor decomposition was found to be relatively fast for sycamore maple and pedunculate oak. Despite the pollution status of the dredged sediment landfill, the sediment substrate was favourable for forest floor decomposition. This might indicate that the soil nutrition status and the high carbonate status override the negative impact of soil pollution with metals and other pollutants. Application of a covering layer composed of uncontaminated soil material resulted in lower Cd concentrations in earthworms, but concentrations were still higher than for references. We conclude from the observations that polluted but fertile soils allow for afforestation and for regular forest floor decomposition with normal or slightly elevated metal concentrations. Only long-term observations of such new forests will lead to a correct site-specific assessment of the actual ecological risks, but after 16 years of landfilling and 12 years of afforestation no adverse effects were observed. 相似文献
120.
Egene Caleb Elijah Sigurnjak Ivona Regelink Inge C. Schoumans Oscar F. Adani Fabrizio Michels Evi Sleutel Steven Tack Filip M. G. Meers Erik 《Journal of Soils and Sediments》2021,21(2):678-688
Journal of Soils and Sediments - This study investigated the C and N mineralisation potential of solid fractions (SFs) from co-digestated pig manure after P-stripping (P-POOR SF) in comparison with... 相似文献