Deposition of gold mining tailings in tropical soils: metal pollution and toxicity to earthworms

Journal of Soils and Sediments - This study consists of the evaluation of metals acute toxicity levels taking into consideration a hypothetic scenario of dam breaking and land deposition of two...     

Reservoir sediment characterisation by diffuse reflectance spectroscopy in a semiarid region to support sediment reuse for soil fertilization

Journal of Soils and Sediments - Soil erosion by water yields sediment to surface reservoirs, reducing their storage capacities, changing their geometry, and degrading water quality. Sediment...     

Short-term changes in the soil carbon stocks of young oil palm-based agroforestry systems in the eastern Amazon

The current expansion of the oil palm (Elaeis guineensis Jacq.) in the Brazilian Amazon has mainly occurred within smallholder agricultural and degraded areas. Under the social and environmental scenarios associated with these areas, oil palm-based agroforestry systems represent a potentially sustainable method of expanding the crop. The capacity of such systems to store carbon (C) in the soil is an important ecosystem service that is currently not well understood. Here, we quantified the spatial variation of soil C stocks in young (2.5-year-old) oil palm-based agroforestry systems with contrasting species diversity (high vs. low); both systems were compared with a ~10-year-old forest regrowth site and a 9-year-old traditional agroforestry system. The oil palm-based agroforestry system consisted of series of double rows of oil palm and strips of various herbaceous, shrub, and tree species. The mean (±standard error) soil C stocks at 0–50 cm depth were significantly higher in the low (91.8 ± 3.1 Mg C ha^?1) and high (87.6 ± 3.3 Mg C ha^?1) species diversity oil palm-based agroforestry systems than in the forest regrowth (71.0 ± 2.4 Mg C ha^?1) and traditional agroforestry (68.4 ± 4.9 Mg C ha^?1) sites. In general, no clear spatial pattern of soil C stocks could be identified in the oil palm-based agroforestry systems. The significant difference in soil carbon between the oil palm area (under oil palm: 12.7 ± 2.3 Mg C ha^?1 and between oil palm: 10.6 ± 0.5 Mg C ha^?1) and the strip area (17.0 ± 1.4 Mg C ha^?1) at 0–5 cm depth very likely reflects the high input of organic fertilizer in the strip area of the high species diversity oil palm-based agroforestry system treatment. Overall, our results indicate a high level of early net accumulation of soil C in the oil palm-based agroforestry systems (6.6–8.3 Mg C ha^?1 year^?1) that likely reflects the combination of fire-free land preparation, organic fertilization, and the input of plant residues from pruning and weeding.     

A practical and reliable procedure for in vitro induction of tetraploid tomato

A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.     

Ruminal and morphometric parameters of the rumen and intestines of sheep fed with increasing levels of spineless cactus (Nopalea cochenillifera Salm-Dyck)

Tropical Animal Health and Production - The objective of this study was to evaluate the ruminal parameters (pH, N-NH3, and microbial protein) and morphometry of the rumen and intestine of sheep fed...     

Morphofunctional description of mucous cells in the gills of the Arapaimidae Arapaima gigas (Cuvier) during its development

The gill structure of the Amazonian fish Arapaima gigas (Cuvier 1829) shows ontogenetic changes during development, particularly due the transition from the aquatic to the obligatory air breathing mode of respiration. However, three main cell types can be found in the gills: mitochondrial rich cells, pavement cells and mucous cells (MCs). The MCs are involved in the secretory pathway. The functions of the secreted molecules include mechanical protection of epithelia, protection against parasites and bacterial infection, and role on ion regulation. In this study, we analysed mucous cell location and mucous cell type, based on pH, during the development of A. gigas. Using samples obtained from the environment, gills were collected and fixed in buffered solution. Histological techniques for the identification of MCs were performed Alcian Blue (AB) and periodic acid‐Schiff (PAS). The results showed the presence of PAS+ and AB+ cells in the whole filament in all examined fish. In animals less than 50 g, few MCs were present, and no differences were observed in AB+ and PAS+ cells. In animals weighing close to 500 g, more PAS+ cells than AB+ cells were observed, and in animals that weighed more than 1,000 g, more AB+ cells than PAS+ cells were observed. These observations may be a result of the ontogenetic changes in the gill epithelia, which can change the osmorespiratory compromise in ion regulation functions as well the glycosaminoglycans secreted by PAS cells, which in large animals can play a role in the protection against parasites and bacterial infection.     

Phage Therapy and Photodynamic Therapy: Low Environmental Impact Approaches to Inactivate Microorganisms in Fish Farming Plants

Owing to the increasing importance of aquaculture to compensate for the progressive worldwide reduction of natural fish and to the fact that several fish farming plants often suffer from heavy financial losses due to the development of infections caused by microbial pathogens, including multidrug resistant bacteria, more environmentally-friendly strategies to control fish infections are urgently needed to make the aquaculture industry more sustainable. The aim of this review is to briefly present the typical fish farming diseases and their threats and discuss the present state of chemotherapy to inactivate microorganisms in fish farming plants as well as to examine the new environmentally friendly approaches to control fish infection namely phage therapy and photodynamic antimicrobial therapy.     

Novel application of square-wave adsorptive-stripping voltammetry for the determination of xanthohumol in spent hops

This paper reports the development of a novel electrochemical assay for xanthohumol (XN) by square-wave adsorptive-stripping voltammetry (SWAdSV) with a hanging mercury drop electrode. The method showed good repeatability (CV < 2%) and linearity (between 10 and 250 μg L(-1)), as well as suitable limits of detection (2.6 μg L(-1)) and quantification (8.8 μg L(-1)). The method was applied for the quantification of this compound in spent hops, and the results obtained were compared with the HPLC-UV method. XN contents determined by the SWAdSV method were 16 ± 1 and 100 ± 4 μg L(-1) for aqueous and methanolic extracts, respectively. The developed new methodology considerably reduces the analysis time, approximately from 25 min (HPLC-UV method) to 7 min, enabling a high sample throughput. In addition, the detection and quantification limits were approximately 5-fold lower than those obtained with the chromatographic method.