全文获取类型
收费全文 | 10277篇 |
免费 | 540篇 |
国内免费 | 975篇 |
专业分类
林业 | 1256篇 |
农学 | 1396篇 |
基础科学 | 714篇 |
1502篇 | |
综合类 | 3369篇 |
农作物 | 539篇 |
水产渔业 | 438篇 |
畜牧兽医 | 1401篇 |
园艺 | 454篇 |
植物保护 | 723篇 |
出版年
2024年 | 65篇 |
2023年 | 176篇 |
2022年 | 447篇 |
2021年 | 507篇 |
2020年 | 489篇 |
2019年 | 465篇 |
2018年 | 298篇 |
2017年 | 479篇 |
2016年 | 330篇 |
2015年 | 496篇 |
2014年 | 463篇 |
2013年 | 576篇 |
2012年 | 732篇 |
2011年 | 769篇 |
2010年 | 638篇 |
2009年 | 555篇 |
2008年 | 531篇 |
2007年 | 602篇 |
2006年 | 441篇 |
2005年 | 433篇 |
2004年 | 199篇 |
2003年 | 185篇 |
2002年 | 138篇 |
2001年 | 138篇 |
2000年 | 201篇 |
1999年 | 235篇 |
1998年 | 181篇 |
1997年 | 152篇 |
1996年 | 147篇 |
1995年 | 148篇 |
1994年 | 99篇 |
1993年 | 99篇 |
1992年 | 84篇 |
1991年 | 71篇 |
1990年 | 62篇 |
1989年 | 42篇 |
1988年 | 30篇 |
1987年 | 28篇 |
1986年 | 14篇 |
1985年 | 12篇 |
1984年 | 6篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1963年 | 2篇 |
1955年 | 1篇 |
1947年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
151.
152.
153.
广西贺街的生态农业旅游资源丰富,文章通过对生态农业旅游的界定,分析了广西贺街开发生态农业旅游的可行性,并提出了相应的开发措施。 相似文献
154.
155.
Susu Liu Jianjun Lyu Qianqian Li Xi Wu Yanwei Yang Guitao Huo Qingfen Zhu Ming Guo Yuelei Shen Sanlong Wang Changfa Fan 《Journal of toxicologic pathology》2022,35(1):25
Lymphoma is the third most common cancer diagnosed in children, and T-cell lymphoma has the worst prognosis based on clinical observations. To date, a lymphoma model with uniform penetrance has not yet been developed. In this study, we generated a p53 deficient mouse model by targeting embryonic stem cells derived from a C57BL/6J mouse strain. Homozygous p53 deficient mice exhibited a higher rate of spontaneous tumorigenesis, with a high spontaneous occurrence rate (93.3%) of malignant lymphoma. Because tumor models with high phenotypic consistency are currently needed, we generated a lymphoma model by a single intraperitoneal injection of 37.5 or 75 mg/kg N-methyl-N-nitrosourea to p53 deficient mice. Lymphoma and retinal degeneration occurred in 100% of p53+/− mice administered with higher concentrations of N-methyl-N-nitrosourea, a much greater response than those of previously reported models. The main anatomic sites of lymphoma were the thymus, spleen, bone marrow, and lymph nodes. Both induced and spontaneous lymphomas in the thymus and spleen stained positive for CD3 antigen, and flow cytometry detected positive CD4 and/or CD8 cells. Based on our observations and previous data, we hypothesize that mice with a B6 background are prone to lymphomagenesis. 相似文献
156.
劳务派遣作为一种新型的用工形式,在农业科研单位广泛应用中取得了明显的效果。然而,由于人员身份的双重性质,劳务派遣用工形式在具体实践中存在同工不同酬、职业规划模糊、归属感缺失等问题。文章基于双因素理论的视角,就如何通过激励手段提高劳务派遣人员工作积极性提出了相应的对策。 相似文献
157.
基于Meta分析和气象因子驱动的苏豫皖小麦籽粒蛋白质含量地理空间分布特征 总被引:1,自引:0,他引:1
为明确苏豫皖地区小麦籽粒蛋白质含量空间分布规律,收集该区域1999-2019年间与小麦籽粒蛋白质含量相关的研究文献及相应地点的气象数据,运用Meta分析方法建立基于气象因子驱动的小麦籽粒蛋白质含量模型,用ArcGIS反距离插值创建预测图,并利用2019年在江苏实际取样测定的小麦籽粒蛋白质数据予以模型验证;最后根据国家小麦品质标准进行小麦品质区划。结果表明,籽粒蛋白质含量随灌浆中期的总日照时数升高而升高,随播种-孕穗、灌浆前中期的总降水量增加而降低。在苏豫皖各地处于同一气象等级条件时,小麦籽粒蛋白质含量整体上从西向东呈降低趋势。采用2019年多点抽样获得的小麦籽粒蛋白质含量进行验证,发现分省拟合模型的效果较好,相对误差在-10%~0之间。苏豫皖三省中,中筋小麦主要分布在江苏省的中北部、安徽省中部少部分地区;中强筋小麦主要分布在江苏省的北部、安徽省的北部、中西部和西南部、河南省的东北部;强筋小麦主要分布在河南省东部、西北部和西南部;江苏省的南部和中部、安徽省的东南部最适宜弱筋小麦的种植。 相似文献
158.
Cryphonectriaceae species cause serious canker diseases on chestnut, oak and eucalypt trees. Recently, canker symptoms with typical orange fruiting bodies were observed on Chinese chestnut and oak trees in Hebei, Hubei, Shaanxi and Shandong Provinces in China. In the present study, isolates of these fungi were identified based on phylogenetic and morphological evidence, and their pathogenicities were tested on detached chestnut (Castanea mollissima) branches. DNA sequence comparisons of the internal transcribed spacer (ITS) regions and two regions of β-tubulin (TUB1/TUB2) indicate that these isolates represent five species in the Cryphonectriaceae, viz. Cryphonectria japonica, Cryphonectria parasitica, Aurantiosacculus castaneae sp. nov., Cryphonectria neoparasitica sp. nov. and Endothia chinensis sp. nov. The sexual morph of Aurantiosacculus is discovered for the first time and can be distinguished from the other genera in Cryphonectriaceae by dark brown ascospores and tubiform appendages at both ends. Cryphonectria neoparasitica sp. nov. is different from the other Cryphonectria species by its aseptate ascospores. Endothia chinensis sp. nov. is the sole species in Endothia infecting the host genus Castanea. Additionally, it is much smaller than E. gyrosa and narrower than E. singularis in ascospores. The inoculation results showed that these five Cryphonectriaceae fungi isolated from chestnut or oak are all pathogenic to tested chestnut branches. Cryphonectria parasitica appears to be the most aggressive fungus, followed by C. neoparasitica sp. nov., C. japonica, E. chinensis sp. nov. and A. castaneae sp. nov. 相似文献
159.
Invasive plants may be more plastic than non‐invasive plants and maintain high fitness under various environmental conditions. Previous studies mainly focused on the comparisons between invasive and native plants, and comparisons between highly invasive and less invasive exotic species are still relatively rare, especially for comparisons at the subspecies level. This study examined the effects of nutrient addition and shading on the performance of the highly invasive Mimosa invisa and its less invasive subspecies M. invisa var. inermis under either isolated or competitive conditions. Nutrient addition increased biomass and plant height and decreased root‐to‐shoot ratio (R/S). Shading decreased biomass and R/S and increased plant height. Under isolated conditions, the two invaders did not differ in R/S, plant height and plasticity of these traits in response to nutrient addition or shading, and the two invaders also did not differ in biomass production under each of the nutrient and light treatments. When the two invaders competed with each other, M. invisa outcompeted M. invisa var. inermis under high soil nutrient conditions, and the two invaders did not differ in performance under other growth conditions. Thus, only considering competition may we find out the difference between highly invasive species and their closely related, less invasive subspecies. Management of M. invisa should focus on habitats with high soil nutrient availability, in which M. invisa is more likely to dominate. 相似文献
160.
韭菜迟眼蕈蚊紫外敏感视蛋白基因的克隆及光强度对其表达量影响 总被引:1,自引:0,他引:1
为明确韭菜迟眼蕈蚊Bradysia odoriphaga紫外敏感视蛋白基因Bo-uv的作用及其与趋光性的关系,利用常规PCR方法克隆获得Bo-uv基因的全长cDNA序列,分析了其敏感视蛋白的氨基酸序列与其它12种昆虫同源蛋白氨基酸序列之间的系统进化关系,运用qPCR技术检测了不同发育阶段、不同组织及不同光强度下Bo-uv基因的相对表达量。结果表明,Bo-uv基因cDNA全长2 757 bp,开放阅读框1 542 bp,编码514个氨基酸。韭菜迟眼蕈蚊紫外敏感视蛋白的氨基酸序列与其它12种昆虫同源蛋白的氨基酸序列一致性为21.93%~43.00%,与橘小实蝇Bactrocera dorsalis的氨基酸序列同源性最高。Bo-uv基因在韭菜迟眼蕈蚊蛹末期、成虫期表达,在成虫头部的相对表达量较高。在0~10 000 lx光强范围内雌、雄成虫体内该基因的相对表达量均呈先增高后降低趋势。与对照相比,1 000 lx光强度下其相对表达量显著升高,10 000 lx时相对表达量显著降低。表明光强度能够有效地调控Bo-uv基因的表达,该基因在韭菜迟眼蕈蚊感知外界光刺激过程中具有重要作用。 相似文献