根癌农杆菌介导转Bt基因苎麻的获得及其抗虫鉴定

采用本研究室建立的根癌农杆菌介导的高效遗传转化体系,转化苎麻优良品种芦竹青,获得了转Bt基因苎麻候选植株。通过PCR和Southern杂交等分子检测,证明Bt基因已经整合到部分候选植株基因组中。选取PCR和Southern杂交均为阳性的部分株系(T₀)种植在大田,对这些植株进行室内抗虫鉴定并考察其主要农艺性状和品质性状,次年对T₁代植株进行PCR和Southern杂交检测。结果表明, 与对照相比,T₀代转Bt基因苎麻植株的抗虫性均强于对照,部分株系的抗虫性显著强于对照植株;且基本保持了亲本的优良性状;T₁代植株中也含有Bt基因,表明Bt基因能稳定遗传,且T₁代植株在大田的抗虫性明显强于非转基因植株。     

一种新型拖拉机提升试验台概念设计

将等效负荷直接加到提升臂上,完成拖拉机在线提升试验。减少试验人员,提高试验效率,增加安全性。     

Analysis on electrically controlled tunable dual-band metamaterials absorber

In order to realize the absorption frequency controlling of metamaterials absorber intelligently,a dual-band metamaterials absorber is designed in this paper firstly,and then the electrorheological fluid is loaded in the dual-band metamaterials absorber,and the absorption frequency of dual-band metamaterials absorber is tunable by changing the electric field strength applied on electrorheological fluid. The results show that the designed metamaterials absorber has two absorption peaks at 7.403 GHz and 17.511 GHz,and the absorption rate is 99.8% and 70% respectively. With the increase of electric field strength,the absorption frequency gradually moves towards lower frequency,and the absorption frequency regulation rate is up to 55%. Therefore,we propose the concept of dual-band intelligent metamaterials absorber.     

Recycling Dosage Analysis of Alkaline Hydrolysis Supernatant ofWAS as Carbon Source for Denitrification

Reaction rate of alkaline supernatant of waste activated sludge (WAS) as carbon sources for denitrification was investigated to determine the recycling dosage preliminarily. WAS was digested under alkaline conditions and batch tests were carried out under different VFA/N ratio at SRT=9d to examine the denitrification rate and choose the optimal ratio. And then a comparative test was carried out for actual sewage wastewater and pure sewage wastewater to investigate its feasibility in recycle and the recycling dosage. It was found that denitrification curve of all ratios could be divided into four stages and the denitrification rate increased obviously with the increase of VFA/N ratio, which was consistent with that of the pH curve. While as the ratio increased beyond 2.47, the denitrification rate increased slowly. Furthermore, when the supernatant was added to the sewage wastewater to some extent, denitrification rate increased significantly and the removal amount of nitrate in two parallel tests were 47.0 and 33.9 mg/L, respectively.     

Transportation and Transformation of Pollutants in a New Type Constructed Wetland

A three stage series operational pilot scale constructed wetland was built for domestic wastewater treatment with inflow of 4.5 m3/d .The mass balance method was adopted to quantify the transportation and transformation of pollutants in constructed wetland during steady state operation.It was shown that the average removal rate of CODcr was 87.14%, in which 68.45% contributed by dissimilation and 18.69% contributed by assimilation.The average removal rate of TN was 60.21% and the contributions of denitrification and microbial assimilation or plant assimilation were 53.64%, 5.39% and 1.18%, respectively.The average removal rate of TP was 59.36% and the contributions of substrates absorption and microbial assimilation or plant assimilation were 43.55%, 12.26% and 3.55%,respectively.In conclusion, microorganisms played a key role in the removal of pollutants and substrates adsorption was the main approach to phosphorus removal.     

浅谈海南地方文献数字化建设 --兼谈我馆地方文献工作

本文探讨了海南地方文献数字化的背景并着重论述了海南地方文献全文数字化的可行性。     

新型污泥减量技术研究

从现阶段剩余污泥的处理与处置投资大、成本高的现状出发,分析了实际污泥减量化的几种污水处理技术,提出了一种以生物砾石为微生物载体的新型污水处理技术,其不仅具有良好的处理效果,而且达到了无剩余污泥排放的目标。     

冷水江市土地利用研究

研究分析了十五期末冷水汀市土地利用现状,总结了土地利用特点及存在问题,预测了未来一定时期内各类用地指标和主要社会经济指标,进而进行土地利用功能分区,提出科学合理利用土地的措施和建议,以促进冷水江市社会经济的快速、健康发展.     

Studies on genetic relationships among Oakleaf loquat,Daduhe loquat and common loquat by RAPD and ISSR

The genetic relationships among oakleaf loquat （Eriobotrya prinoides）, Daduhe loquat （Eriobotrya prinoides var. dadunensis） and common loquat （Eriobotrya japonica） were studied by RAPD and ISSR markers. The results showed that the similarity coefficient between oakleaf loquat and common loquat was minimal （0.6996 and 0.7219, respectively） and the similarity coefficient between oakleaf loquat and Daduhe loquat was maximal （0.8403 and 0.8211, respectively）, while the similarity coefficient between Daduhe loquat and common loquat was always intermediate （0.7195 and 0.7219, respectively）. The highest additivity was obtained when Daduhe loquat was regarded as the undetermined hybrid （44.9% and 45.8%, respectively）. The specific bands of oakleaf loquat and common loquat were present in Daduhe loquat. Thus, it could be concluded that Daduhe loquat was a hybrid of oakleaf loquat and common loquat.     

试论图书馆的文化责任

图书馆是构建社会主义和谐社会,建设和谐文化的重要组成部分。从图书馆文化责任的内涵出发,分析了图书馆在构建和谐社会中的重要性,论述了如何充分发挥图书馆的文化责任,为构建和谐社会出力献策。