NAA和2，4ˉD对东方百合组织培养的影响

借助正交回归多项式建立数学模型的方法,研究了NAA对东方百合“蒂伯”（Ti ber）鳞片 诱导小鳞茎及2,4ˉD对小叶片诱导愈伤组织的影响,以探讨东方百合组织培养中NAA及 2,4ˉD 的用量范围及最佳用量。结果表明, NAA诱导离体鳞片外植体产生小鳞茎的用量范围为 0.15～ 0.9 mg·L -1 ,最佳用量为0.69 mg·L -1 ; 2,4ˉD诱导叶片形成愈伤组织的最佳用量为3.2 mg· L -1 。     

Research on Shearing Properties of Asphalt Mixtures Composed of Different Gradation Types

In order to assess the shearing properties, some types of mixtures with different aggregate gradations are subjected to the single penetration test. The results show that the shearing properties of the mixtures are obviously influenced by the gradations.     

High density lipoprotein subclasses and activities of protein kinase C

AIM: To investigate the relationship between high density lipoprotein (HDL) subclasses and the binding activities of hepatic cell and extra-hepatic cell HDL receptor and intracellular protein kinase C (PKC). METHODS: Using purified pre-β1 HDL and apoE-deficent HDL3 to react with human smooth muscle cells (SMC) and HepG2 cells, then the activities of pre-β1HDL and apoE-deficent HDL3 binding to SMC and HepG2 cells, and the effects of this two HDL subclasses on PKC activities in SMC and HepG2 cells were observed . RESULTS: The results showed that the value of Kd of pre-β1HDL binding to SMC HDL receptor was not only significantly lower than that of apoE-deficient HDL3 (P<0.05), but significantly lower than pre-β1 HDL binding to HepG2 HDL receptor (P<0.05). Cell PKC system was all activated by binding of the two HDL subclasses with SMC HDL receptor, and the effect of pre-β1HDL appeared to be stronger than apoE-deficent HDL3. No change in HepG2 cell PKC activity by binding the two HDL subclasses with HepG2 HDL receptor was observed. CONCLUSIONS: The results indicate that the ability of pre-β1HDL to promote efflux of cholesterol from extra-hepatic cells is stronger than that of apoE-deficent HDL3, and it seems that plasma pre-β1 HDL mainly interacts with extra-hepatic cell HDL receptor, subsequently, activates PKC signal transduction system and promotes the intracellular cholesterol efflux from cells.     

HPLC法测定木豆中牡荆苷和异牡荆苷含量

建立了测定木豆叶、茎、根中牡荆苷和异牡荆苷含量的高效液相色谱分析方法.色谱柱为HIQ Sil C18V(250 mm × 4.6 mm,5 μm);流动相为甲醇-水-甲酸(体积比35:64.74:0.26); 检测波长330 nm;流速1 mL/min;进样量10 μL; 柱温30 ℃.在2～200 mg/L 牡荆苷、异牡荆苷质量浓度与色谱峰面积线性关系良好; 牡荆苷峰面积精密度的相对标准偏差(RSD)最大为2.93 %,重复性RSD为2.91 %,加样回收率为97.38 %; 异牡荆苷峰面积精密度的RSD最大为2.69 %,重复性RSD为3.37 %,加样回收率为98.63 %.木豆叶、茎、根中牡荆苷、异牡荆苷的质量分数分别为0.768、 0.066、 0.183 mg/g 和0.799、 0.139、 0.013 mg/g.叶中牡荆苷和异牡荆苷含量明显高于茎、根中的含量,可以作为大规模获得牡荆苷、异牡荆苷的资源.     

阿德福韦与七元瓜环超分子相互作用的研究

利用紫外吸收光谱法(UV-Vis)、红外光谱法(IR)及差示扫描量热法(DSC)等考察了七元瓜环(Q[7])与阿德福韦(PMEA)的相互作用.结果表明:Q[7]与PMEA相互作用形成包结比为1∶1的主-客体包合物,包结平衡常数为(2.81±0.04)x104L/mol;在0＜pH＜6范围内均能观察到主客体的相互作用,在...     

河西走廊孜然套种大豆密度试验

以大豆品种中黄30号和孜然品种新抗18-2为材料,在河西灌区进行了孜然套种大豆密度试验研究。结果表明,当套种总带幅为160 cm,大豆带幅80 cm种3行,行株距30 cm×20 cm;孜然带幅80 cm,撒播密度为225.0万株/hm2时优势最明显,带田总产量可达9 478.8 kg/hm2,较对照增产8.8%,产值和纯收益分别为54 351元/hm2和48 921元/hm2,较对照增加10.7%和12.10%,且稳定系数高,为最佳套种密度。     

四子王旗景观格局演变与沙化土地动态变化分析

利用RS和GIS的空间分析技术,通过提取分析四子王旗两期遥感影像数据,结合现地调查,对区域景观格局演变和沙化土地动态变化进行分析。结果表明:从2003年到2008年5a间,景观类型和沙化土地类型发生了较为明显的变化,主要表现在:林地景观面积明显增加,耕地、草地和未利用地明显减少,林地面积增加了41 361hm2,耕地、草地和未利用地面积分别减少了14 561、9 427hm2和19 307hm2;沙化土地5a间减少了664hm2,其中半固定沙地减少了261hm2,固定沙地增加了322hm2,露沙地减少了512hm2,沙化耕地减少了153hm2,风蚀劣地减少了60hm2。分析认为近年来实施的京津风沙源治理工程是区域景观演变和沙化土地动态变化的主要因素。     

鲜切芹菜表面微生物污染及检测

通过对鲜切芹菜表面微生物的检测,发现从原料到成品各环节存在不同程度微生物污染,为建立鲜切芹菜安全卫生质量控制体系提供参考依据。     

提高猪场免疫效果的几点措施

猪场免疫效果好坏是关系到养猪成败的关键环节之一.文章从疫苗的质量、保存及使用方法等方面分析了猪场免疫失败的原因,并针对问题提出了几点提高猪场免疫效果的措施.     

Constructing the barley model for genetic transformation in Triticeae

Barley(Hordeum vulgare L.) is one of the oldest domesticated crops, showing dramatic adaptation to various climate and environmental conditions. As a major cereal crop, barley ranks the 4th after wheat, maize and rice in terms of planting area and production all over the world. Due to its diploid nature, the cultivated barley is considered as an ideal model to study the polyploid wheat and other Triticeae species. Here, we reviewed the development, optimization, and application of transgenic approaches in barley. The most efficient and robust genetic transformation has been built on the Agrobacterium-mediated transfer in conjunction with the immature embryo-based regeneration. We then discussed future considerations of using more practical technologies in barley transformation, such as the T-DNA/transposon tagging and the genome editing. As a cereal crop amenable to genetic transformation, barley will serve as the most valuable carrier for global functional genomics in Triticeae and is becoming the most practical model for generating value-added products.