高体鳑鲏幼鱼集群行为的研究

为了考查高体鳑鲏(Rhodeus ocellatus)幼鱼的集群行为,研究选取248尾幼鱼,组成1、2、4、8和16尾高体鳑鲏幼鱼的群体(N = 8),在(25 ± 1)℃水温条件下进行15分钟的视频拍摄、运动轨迹提取和分析。结果显示：①高体鳑鲏的自发游泳速度多介于0-10 cm/s之间,其速度中位数随群体大小上升呈先下降后上升的趋势(P ﹤ 0.05),但各组间游泳速度的同步性随群体大小的上升而下降(P ﹤ 0.05);②焦点鱼和群体中其它个体的最近邻距离(NND)多出现在0-10 cm之间,且2和4个体组的NND显著大于8和(或)16个体组(P ﹤ 0.05);③2和4个体组排列的极性显著高于8和16个体组(P ﹤ 0.05)。研究结果表明群体大小对高体鳑鲏集群行为的特征有显著影响,随群体大小上升,凝聚力上升,但协调性有所下降。     

IBA对芍药扦插生根的影响及生根过程中相关酶活性的变化

以芍药‘大富贵’品种为材料, 用1 000、2 000和3 000 mg·L^-1 3个浓度的IBA处理进行扦插对比试验, 研究其生根过程中过氧化物酶( POD) 、多酚氧化酶( PPO) 、吲哚乙酸氧化酶( IAAO) 活性的动态变化。结果表明: IBA处理极显著地提高了插穗的生根率、根数和总根长, 其中以2 000 mg·L ^-1为IBA最佳生根浓度, 将生根周期缩短18 d, 并明显提高了生根质量。在生根过程中, 插穗中POD、PPO活性分别呈现“缓慢上升→下降→急剧上升→下降”和“降→升→降”的变化趋势, 但与对照组相比, 处理组的变化更为剧烈, 峰值出现提前了18 d。IAAO活性在对照和处理中呈现不同的变化趋势: 前者为“升→降→升”, 而后者为“降→降→升”。此外, 高活性的POD、PPO有利于不定根的形成, 低活性的IAAO则促进生根。     

Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

银杏叶对辣椒植株发酵液自毒作用的化感调控

采用1/2霍格兰（Hoagland）营养液水培法,研究辣椒植株发酵液的自毒作用及银杏叶发酵液和 再腐解液对其的调控作用。结果表明,辣椒植株发酵液使辣椒受到自毒作用,根系活力下降,相对电导 率增大。银杏叶发酵液加重了辣椒植株发酵液的自毒作用,并且与其浓度成正相关。银杏叶再腐解液缓 解了辣椒的自毒作用,缓解效果与再腐解液的浓度有关。     

Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation

AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory.     

钾素不同用量对莴苣硝酸盐积累的影响

以莴苣为试材,采用通用随机排列设计,利用基质培育方法,研究不同供应水平的钾素营养对莴苣硝酸盐积累的影响.结果表明:在供试条件下,硝酸盐含量与硝酸还原酶活性在一定浓度范围内都随着钾素浓度的增大而降低.     

干花染色技术研究

研究了云南省常见的几种漂白过的花材的染色工艺.结果表明:染液的浓度增加,染色效果显著,染液浓度为3‰时可获得满意效果.温度升高及时间增加,有利于花材的染色,当温度达到70～90℃时染色效果最佳,染液pH在4～6之间为佳.     

LED灯补光对萝卜生长及光合特性的影响

为了解发光二极管(LED)补光对植物生长及光合特性的影响,选择萝卜Raphanus sativus 作为材料,将其栽种于2种LED灯补光和温室自然条件下30 d后测定其生长指标及光合特性.结果表明:与自然光相比,使用LED灯补光的萝卜在叶片数、叶片长、宽等指标上并无差异,但有利于肉质根的形成,肉质根鲜重分别增加了5.93 g/株和10.93g/株;LED灯补光使萝卜光补偿点和光饱和点升高,光合能力增强,但胞问二氧化碳浓度、蒸腾速率和气孔导度差异不明显.     

佳玉苦瓜丰产稳产性分析

利用方差分析法和高稳系数(HSC)法,对2008-2009年福州地区常见的苦瓜品种多点比较试验的产量结果进行了丰产性和稳产性分析.结果表明,佳玉苦瓜平均折合产量最高为3 008.4 kg/667 m2,比对照增产10.41%;产量主效应值最高,为2.314,高稳系数最大,为95.25.综合评价认为,佳玉苦瓜丰产性显著,稳定性好,适应性广,可加强示范和研究,以便进一步在福建省进行推广.     

灵芝多糖提取物胶囊对小鼠免疫功能的影响

通过设3个浓度的药物组和空白对照组,分别进行脏器指数测定,ConA诱导的小鼠脾淋巴细胞转化试验,绵羊红细胞诱导小鼠DTH试验,小鼠血清溶血素测定,抗体生成细胞检测,小鼠腹腔巨噬细胞吞噬鸡红细胞试验,小鼠碳廓清试验,NK细胞活性测定试验。结果表明,灵芝多糖提取物胶囊能增加ConA诱导的小鼠脾淋巴细胞的增殖能力和小鼠左后足跖部厚度差24 h测量值,提高小鼠血清溶血素抗体积数和溶血空斑数。表明灵芝多糖提取物胶囊具有较强的免疫调节作用。